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Method for measuring form of selenium in selenium-rich spiral seaweed

A spirulina and selenium-enriched technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low extraction rate and undetectable, etc., and achieve the effects of increasing specific surface area, ensuring stability, and mild reaction conditions

Inactive Publication Date: 2016-01-20
SUZHOU INST FOR ADVANCED STUDY USTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to establish a method specially used for the determination of selenium speciation in selenium-enriched spirulina, aiming to fill the gap in the selenium-enriched spirulina selenium speciation method, and to solve the problem that the extraction rate of other selenium-measuring morphology methods is not high or cannot be obtained. Checked out issue

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Determination of selenium morphology of selenium-enriched Spirulina platensis sample

[0043] The selected selenium-enriched Spirulina platensis has a total selenium content of 1003mg / kg (DW).

[0044] (1) Sample pretreatment: take 0.1g of ground dried selenium-enriched spirulina and add 5ml of 0.1mol / L phosphate buffer for ultrasonic extraction;

[0045] (2) Enzymatic hydrolysis of sample wall breaking: adjust the pH value of the pre-treated sample system to 5-7, then add 50mg of lysozyme to it, and then place the centrifuge tube horizontally in a constant temperature shaking shaker at 30~50℃ Cultivate for 12-18h.

[0046] (3) Sample enzymolysis: adjust the pH value of the above reaction system to 7.5, then add 5mg of proteinase K, and then place the centrifuge tube horizontally in a constant temperature shaking shaker at 50°C for 8-12h.

[0047] (4) Enzymatic hydrolysis after sample separation: Centrifuge the above-mentioned enzymatically hydrolyzed sample in a refr...

Embodiment 2

[0054] Example 2: Example of selenium speciation determination of selenium-enriched Spirulina platensis sample

[0055] The selected selenium-enriched Spirulina platensis has a total selenium content of 12mg / kg (DW).

[0056] (1) Sample pretreatment: Take 0.2g of ground dry sample of Se-enriched Spirulina and add 5ml 0.2mol / LTris-HCl buffer for ultrasonic extraction;

[0057] (2) Enzymatic hydrolysis of sample wall breaking: Adjust the pH value of the pre-treated sample system to 5-7, then add 10mg of lysozyme to it, and then place the centrifuge tube horizontally in a constant temperature shaking shaker at 30-50°C Cultivate for 12-18h.

[0058] (3) Sample enzymolysis: adjust the pH of the above reaction system to 7.5, then add 1 mg of proteinase K, and then place the centrifuge tube horizontally in a constant temperature shaking shaker at 50°C for 8-12 hours.

[0059] (4) Enzymatic hydrolysis after sample separation: Centrifuge the above-mentioned enzymatically hydrolyzed sample in a ...

Embodiment 3

[0066] Example 3: Determination of selenium speciation in the sample of selenium-enriched Spirulina salina

[0067] The total selenium content of the selected selenium-enriched Spirulina salina is 117mg / kg (DW).

[0068] (1) Sample pretreatment: take 0.2g of ground dry sample of selenium-enriched Spirulina salina and add 5ml of 0.1mol / L phosphate buffer for ultrasonic extraction;

[0069] (2) Enzymatic hydrolysis of sample wall breaking: adjust the pH value of the pre-treated sample system to 5-7, then add 25mg of lysozyme to it, and then place the centrifuge tube horizontally in a constant temperature shaking shaker at 30~50℃ Cultivate for 12-18h.

[0070] (3) Sample enzymolysis: adjust the pH of the above reaction system to 7.5, then add 2mg of proteinase K, and then place the centrifuge tube horizontally in a constant temperature shaking shaker at 50°C for 8-12h.

[0071] (4) Enzymatic hydrolysis after sample separation: Centrifuge the above-mentioned enzymatically hydrolyzed sample...

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Abstract

The invention discloses a method for measuring the form of selenium in selenium-rich spiral seaweed. Wall breaking treatment is carried out on a selenium-rich spiral seaweed sample through lysozyme, then the treated sample is subjected to enzymolysis in a substep continuous extracting mode, and after enzymatic hydrolysate obtained through enzymatic reactions at all steps passes through a film, the form of selenium is measured through an HPLC-HG-AFS upper computer. The method is used for measuring the form of selenium in the selenium-rich spiral seaweed sample, the extraction ratio is high, the form of selenium of the sample is stable, and the form of selenium cannot change in the reaction process.

Description

Technical field [0001] The invention relates to a method for determining the selenium form in selenium-enriched spirulina, belonging to the field of analysis and testing of selenium. Background technique [0002] Selenium is an essential trace element for the human body and an essential component of glutathione peroxidase. Selenium has an antioxidant effect and is closely related to human health. Selenium has anti-cancer, heart protection, anti-hepatic necrosis, prevention and treatment of myopia and cataracts. It has various pharmacological effects such as detoxification, improving immunity, delaying aging and enhancing reproductive function. According to incomplete statistics, there are more than 40 countries and regions in the world that are short of selenium, and 72% of my country's regions are selenium-deficient areas. Therefore, the search for and development of selenium-enriched products to ensure adequate intake of selenium is being used by all countries. Scientific worke...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 尹雪斌袁林喜侯遇珠
Owner SUZHOU INST FOR ADVANCED STUDY USTC
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