The invention provides a high-iron low-grade nickel sulfide ore selective biological leaching process. The process comprises the following steps of stirring and leaching, selective leaching, deironing, extraction, reverse extraction and electrodeposition, then obtaining a cathode nickel. According to the process, in particular with respect to a flotation nickel sulfide ore concentrate with high iron content and low nickel grade, a stirring biological leaching process is adopted, measures for controlling growth, pH value, temperature and ventilatory capacity of vulcanized bacteria are adopted,redox potential in the leaching process is controlled, dissolution of iron-containing sulfide minerals such as pyrite is effectively inhibited, and selective leaching of the nickel-containing sulfidemineral is realized. The process is short in procedure, simple in equipment, saving in investment, low in cost, free of pollution, high in valuable metal recovery rate and capable of treating low-grade nickel sulfide ore resources which cannot be treated by a pyrogenic process smelting process, utilization range of resources can be expanded, comprehensive recovery level of the nickel metal is improved, and economic benefits, social benefits and environmental benefits of the process is remarkable.