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A strain of Penicillium oxalicum and its cultivation method and application

A Penicillium oxalicum, culture method technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problem of less fungus, achieve strong tolerance, reduce process scale, and reduce investment costs Effect

Active Publication Date: 2022-06-07
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, research on microorganisms that are tolerant and efficient in leaching lithium cobaltate mainly focuses on bacteria, such as Acidithiobacillus ferrooxidans, etc. Penicillium oxalicum containing lithium cobaltate and its application in lithium battery recycling have not been reported in China

Method used

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  • A strain of Penicillium oxalicum and its cultivation method and application
  • A strain of Penicillium oxalicum and its cultivation method and application
  • A strain of Penicillium oxalicum and its cultivation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Isolation and acclimation screening to obtain Penicillium oxalicum SYJ-1:

[0028] (1) Collect soil from the surface layer of the sewage outlet of the lithium battery factory in Mianyang City, Sichuan Province, immediately store it at low temperature and bring it back to the laboratory, take 1 cm 3 The soil was in 100mL sucrose medium (medium composition: sucrose (100g / L), yeast extract powder (1.6g / L), NaNO 3 (1.5g / L), KH 2 PO 4 (0.5g / L), MgSO 4 ·7H 2 O (0.025g / L), KCl (0.025g / L)), and 1g / L lithium cobalt oxide powder was added to it, and cultured in a constant temperature shaking shaker at 30°C and 150r / min.

[0029] (2) After culturing for two days, the liquid shake flask supernatant was -6 , 10 -8 , 10 -10 , 10 -12 The concentration gradient was diluted, and then inoculated on a sucrose medium plate containing 2% agar, placed in a 32°C incubator for five days, and fungal colonies were picked for further screening and purification.

[0030] (3) The picked fu...

Embodiment 2

[0034] Identification of strains:

[0035] The ITS sequence of the strain was extracted with the Ezup column fungal genomic DNA extraction kit, and PCR amplification was performed to obtain an amplified product with a length of about 600 bp, which was then sequenced by a sequencing company. BLAST alignment of the detected sequence with the sequence in GenBank database shows that the strain has high homology with Penicillium (Penicillium), and the similarity with Penicillium oxalicum is more than 99%. Combined with the morphological characteristics, culture characteristics and ITS sequence analysis results, it was determined that SYJ-1 was Penicillium oxalicum.

Embodiment 3

[0037] Determination of tolerance of Penicillium oxalicum SYJ-1 to lithium cobaltate:

[0038] With eleven concentrations of 1g / L, 3g / L, 5g / L, 7g / L, 10g / L, 20g / L, 40g / L, 60g / L, 80g / L, 100g / L and 200g / L Lithium cobaltate tolerance experiments of SYJ-1 were performed in sucrose medium of lithium cobaltate. The above strain SYJ-1 was inoculated on a red Bengal plate, and after five days of culture, the spore suspension was prepared with normal saline, and the concentration of the spore suspension was determined by hemocytometer to be 9.6 × 10 4 spores / mL, according to the inoculum volume of 500 μL / bottle, inoculated into the above eleven 100 mL sucrose mediums containing lithium cobaltate with different contents, and placed in a constant temperature shaking shaker at 30 ° C and 150 r / min for culture, and cultured for 7 Days later, the formation of mycelial balls in the shake flasks was observed.

[0039] The results showed that the growth of strains existed in the shake flasks ...

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Abstract

The present invention provides a strain of Penicillium oxalicum SYJ-1, which is classified as Penicillium oxalicum (Penicillium oxalicum), and has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on November 7, 2018. The preservation number is: CGMCC No. 16497. The Penicillium oxalicum of the present invention has a strong leaching effect on lithium cobalt oxide, an important component in waste lithium batteries, and can efficiently recycle lithium and part of cobalt at the same time under the condition of relatively high solid-liquid ratio, and is expected to achieve selective leaching. And to the common heavy metal Fe 2+ 、Ni 2+ , Mn 2+ 、Cu 2+ And lithium cobalt oxide has strong resistance, which further expands its application range. The invention provides excellent bacterial strain resources for the future development of bacterial agents that are resistant to heavy metals and efficiently recover cobalt and lithium in waste lithium batteries, and have great practical application significance.

Description

technical field [0001] The invention relates to environmental biotechnology, in particular to a strain of Penicillium oxalicum tolerant to high-content heavy metals. Background technique [0002] With the development of society, the way of life of human beings has changed, and the demand for electrical and electronic equipment is increasing. Lithium batteries are widely used in energy storage devices of various electronic and electrical equipment due to their advantages of high voltage, wide operating temperature range, and ideal discharge resistance. However, after a lithium-ion battery is used for a period of time, the electrode material will expand or shrink, resulting in poor battery performance and eventually being discarded. A large number of discarded lithium batteries may not only cause serious environmental pollution and great waste of resources, but also pose serious safety hazards. Therefore, it is urgent to find efficient and reasonable treatment technologies. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C22B3/18C22B26/12C22B23/00C22B7/00C12R1/80
CPCC12N1/14C22B3/18C22B26/12C22B23/0407C22B7/006C12R2001/80C12N1/145Y02P10/20
Inventor 周豪祁晓妍唐汇东
Owner DALIAN UNIV OF TECH
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