52results about How to "Excellent strain resources" patented technology

The invention discloses a strain of bacillus cereus which is preserved in China General Microbiology Center of Committee for Culture Collection of Microorganisms since 31st December, 2014, and the preservation number is CGMCC No. 7069. The bacillus cereus L90 provided by the invention is capable of improving the drought resistant capability of juglans regia. Experiment research shows that after the bacillus cereus provided by the invention is inoculated to the surround of juglans regia roots and juglans regia is stressed by drought for a certain time, juglans regia is substantially improved in bacterial community structure diversity in rhizosphere soil compared with juglans regia inoculated with common growth-promoting bacteria, the leaf relative water content and the water utilization efficiency are improved, and the drought resistance of the plant is enhanced. Therefore, the bacillus cereus provided by the invention or a bacterium preparation by taking bacillus cereus as a raw material can be used as an inoculation agent and applied to drought environment, and the bacillus cereus provided by the invention helps to provides an excellent bacterial strain resource for improving the drought resistance of juglans regia.

The invention relates to the field of biology, in particular to novel staphylococcus aureus bacteriophages as well as a composition and application thereof, and provides four staphylococcus aureus bacteriophages, including podoviridae BP-13 with the preservation number of CCTCC NO: M 2015142. staphylococcus aureus BP-13A with the preservation number of M 2016535; podoviridae BP-14 with the preservation number of NO: M 2015143; and podoviridae AHJD-like phage BP-39 with the preservation number of NO: M 2015144. The four bacteriophages are strict virulent bacteriophages, can effectively kill various staphylococcus aureus such as MRSA, MSSA, BORSA and the like, have a wide host range, but have no toxic effect on normal microbial flora, and the DNA of the bacteriophages cannot encode virulence genes. Therefore, the bacillus subtilis can be used for preventing or treating human and livestock bacterial infection caused by the staphylococcus aureus, provides an excellent strain resource for developing a novel antibacterial preparation, and has a good application and development prospect. In addition, the host range and the antibacterial efficiency of the cocktail mixture disclosed by the invention are further greatly improved.

The invention discloses a strain of Serratia marcescens, which is classified as Serratia sp. and has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on January 11, 2016, and the preservation number is CGMCC NO.11987. The Serratia marcescens of the present invention has an obvious inhibitory effect on A549 non-small cell lung cancer and S180 ascites tumor, and the curative effect is very outstanding. Therefore, the Serratia marcescens can be used as a raw material to prepare a drug for inhibiting tumors, For example: culture the bacteria to obtain a culture, and add or not add other drugs, auxiliary materials, etc. to process and prepare the bacterial agent. The invention provides an excellent strain resource for studying the tumor suppressing mechanism, application, drug development and the like of the Serratia bacterium.

The invention discloses acinetobacter calcoaceticus for promoting rhizosphere growth of walnuts. The acinetobacter calcoaceticus is named acinetobacter calcoaceticus (Acinetobacter calcoaceticus) X128 in class, and preserved at the general microbiological center of the China Committee for Culture Collection of Microorganisms on December 31, 2012; and the preservation number is CGMCC No.7071. The invention also provides application of the acinetobacter calcoaceticus for promoting rhizosphere growth of the walnuts. The acinetobacter calcoaceticus for promoting rhizosphere growth of the walnuts is evenly fed to the periphery of the walnut root in a pouring manner; root growth can be obviously facilitated; the quantity of lateral roots is improved; and growth of the bottom diameter of the walnut and the height of seedling is facilitated. In contrast, the difference reaches a significant level. Therefore, excellent strain resources are provided for developing a special microbial fertilizer for the walnut in the future.

The invention discloses a kind of Burkholderia pyrrocinia, which is classified as Burkholderia pyrrocinia (Burkholderia pyrrocinia), and the strain number is LWK2, which has been preserved in the China Type Culture Collection Center CCTCC, and the preservation number is : CCTCC NO: M 2016007, date of deposit: January 5, 2016. The invention also discloses the application of the above-mentioned Burkholderia pyrrole in promoting the growth of the japonica. The LWK2 strain has strong potassium-decomposing ability and auxin secretion ability; it can obviously promote the germination of cucumber seeds; the LWK2 bacterial agent was inoculated with the seedlings of Lianxiang tree, and the results showed that the bacterial agent can obviously promote the growth of Lianxiang tree seedlings. At the same time, the strain has a certain antagonistic effect on plant pathogens such as rice blast fungus, ascosporium chrysogenum, Fusarium oxysporum, rice sheath blight fungus and rhizoctonia.

The invention discloses a Pseudomonas putida, which is classified as Pseudomonas putida and has a strain number of LWPZF. It has been preserved in the China Center for Type Culture Collection CCTCC, and the preservation number is CCTCC NO: M 2016008. The deposit date is January 5, 2016. The invention also discloses the application of the above-mentioned Pseudomonas putida in promoting the growth of the japonica. The LWPZF strain has a strong ability to decompose inorganic phosphorus and auxin secretion; it can significantly promote the germination of cucumber seeds; the LWPZF bacterial agent was inoculated with the seedlings of Lianxiang tree, and the results showed that the bacterial agent can significantly promote the growth of Lianxiang tree seedlings. At the same time, the strain has a certain antagonistic effect on plant pathogens such as Fusarium oxysporum, rice sheath blight, Rhizoctonia and rice blast fungus. Therefore, the present invention provides excellent bacterial strain resources for the development of the special biological fertilizer for the syringa japonica.

The invention discloses an endophytic Bacillus amyloliquefaciens JL‑B05 and its application, belonging to the technical field of microorganisms. Endophytic Bacillus amyloliquefaciens JL‑B05 taxonomically named Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) JL‑B05, which was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms on November 22, 2019, with the deposit number: CGMCC No.18998. The bacterial strain can effectively inhibit the mycelium growth and spore germination of edible fungus pathogenic bacteria. The volatile substances of the strain have a strong inhibitory effect on the growth of pathogenic mycelia. The bacterial strain has great application value in the prevention and treatment of edible fungus pathogenic bacteria.

The invention discloses a serratia marcescens FXW-NJ2D, which is classified and named as serratia marcescens subsp marcescens. The serratia marcescens is preserved in China Centre for Type Culture Collection with the preservation number of CCTCC M 2012261. The invention further discloses an application of the serratia marcescens for promoting the growth of Chinese sweetgum. Under the condition ofliquid shake-flask culture, the bacterial strain in the invention has a strong dissolving effect on insoluble phosphates such as tricalcium phosphate, iron phosphate, aluminium phosphate and hydroxyapatite; the bacterial stain is prepared into a fungicide, and the fungicide is inoculated in a Chinese sweetgum seedling; the results indicate that the fungicide can obviously promote the growth of Chinese sweetgum; and therefore, an excellent bacterial strain resource is provided for developing a special microbial fertilizer for Chinese sweetgum in the future.

The invention discloses a tea tree growth-promoting rhizobacterium of which the classification designation is Serratia marcescens. The strain number is JW-CZ2. The Serratia marcescens is collected by China Center for Type Culture Collection, the collection number is CCTCC M 2015095, and the collection date is March 10th, 2015. The invention also discloses application of the Serratia marcescens in promoting tea tree growth. The JW-CZ2 strain has high solubility for insoluble phosphates, such as tricalcium phosphate, magnesium phosphate and iron phosphate. The JW-CZ2 strain has IAA (indoleacetic acid) secretion capacity and ACC deaminase producing capacity. When a tea tree seedling plant is inoculated in the JW-CZ2 microbial inoculant, the microbial inoculant can obviously promote the growth of the tea tree seedling. Therefore, the tea tree growth-promoting rhizobacterium Serratia marcescens can provide an excellent strain resource for future development of special microbial fertilizers for tea trees.

The invention belongs to the field of biotechnology, and in particular relates to a broad-spectrum colibacillus phage and its application. The Escherichia coli phage WXLECP008 (Escherichia coli phage, WXLECP008) provided by the present invention is a virulent phage, which has tolerance at pH 4 to pH 10 and at -80°C to 65°C, cultivated for 8 hours under the condition of MOI=0.1, and its titer is 3.5×10 10 pfu / mL. The Escherichia coli phage provided by the invention is a broad-spectrum phage with a wide cleavage range, has high titer and high safety, can reproduce in large quantities in non-pathogenic host bacteria, and can be used for large-scale fermentation production. The phage described in the invention can provide excellent bacterial strain resources for the development of phage therapy, has good application and development prospects, and is suitable for popularization and application.

The present invention relates to a Gorgon fruit rhizosphere growth-promoting bacterium, which is classified as Baclicus lincheniformis Q67, preserved in the China Center for Typical Culture Collection, and the preservation number is CCTCC NO: M2014652. The Gorgon fruit rhizosphere growth-promoting bacteria of the present invention Bacteria Q67 can significantly promote the growth and development of Gorgon seedlings, and increase the content of available nitrogen in the soil environment where Gorgon grows. The present invention also relates to the application of the above-mentioned rhizosphere growth-promoting bacteria of Gorgon fruit in the preparation of bacterial agent, and the result shows that the bacterial agent can obviously improve the germination rate of Gorgon fruit seed.