52results about How to "Excellent strain resources" patented technology

The invention relates to the field of biology, particularly to novel Staphylococcus aureus phages and a composition thereof, and applications of the composition, and provides 4 Staphylococcus aureus phages such as Podoviridae sp .BP-13 with the preservation number of CCTCC NO:M2015142, Staphylococcus aureus phage BP-13A with the preservation number of CCTCC NO:M2016535, Podoviridae sp .BP-14 withthe preservation number of CCTCC NO:M2015143, and Podoviridae picovirinae Ahjdlikevirus BP-39 with the preservation number of CCTCC NO:M2015144. According to the present invention, the four phages arethe strict potent phages, can effectively kill various types of Staphylococcus aureus such as MRSA, MSSA, BORSA and the like, have wide host ranges, and have no toxic effect on normal microbial flora, and the DNA of the phages cannot encode virulence genes, such that the Staphylococcus aureus phages can be used for preventing or treating human and animal bacterial infections caused by Staphylococcus aureus, can provide excellent strain resources for the development of novel antibacterial preparations, and have good application development prospects; and the host range and the antibacterial efficiency of the cocktail mixture of the present invention are further greatly improved.

The invention relates to the field of microorganisms, and specifically provides a novel staphylophage and a composition, as well as preparation methods and application thereof. The phage is aureus staphylophage J1P1 with the storage number of CCTCC NO: M2016284, aureus staphylophage J1P2 with the storage of CCTCC NO: M2016285, aureus staphylophage J1P3 with the storage of CCTCC NO: M2016286, aureus staphylophage J2-1P1 with the storage of CCTCC NO: M2016287, or aureus staphylophage J2-1P2 with the storage of CCTCC NO: M2016288. The phage is capable of preventing and treating human or animal bacterial infectious diseases caused by various staphylococcocci, particularly by aureus staphylophage; a good phage resource is provided to develop novel antimicrobial preparations; the novel staphylophage has a good application and development prospect.

The invention discloses Burkholderia pyrrocinia of which the classification name is Burkholderia pyrrocinia. The strain number is LWK2, the Burkholderia pyrrocinia is preserved in the China center for type culture collection (CCTCC), the preservation number is CCTCC NO: M 2016007, and the preservation date is January 5, 2016. The invention further discloses application of the Burkholderia pyrrocinia in cercidiphyllum japonicum growth promotion. The LWK2 strain has the strong potassium decomposing capacity and auxin secretion capacity and can obviously promote germination of cucumber seeds; when an LWK2 bactericide is inoculated to a cercidiphyllum japonicum seedling, the result shows that the bactericide can obviously promote growth of the cercidiphyllum japonicum seedling. Meanwhile, the strain has a certain antagonistic effect on phytopathogen such as pyticularia oryzae, cytospora chrysosperma, fusarium oxysporum, riziocotinia solani and rhizoctonia.

The invention discloses efficient phosphate-solubilizing penicillium pinophilum of laxus malrel rhizosphere. The classified name of the efficient phosphate-solubilizing penicillium pinophilum of laxus malrel rhizosphere is penicillium pinophilum CBW10, and the penicillium pinophilum is collected in the CCTCC (China Center for Type Culture Collection) and has the collection number of CCTCC No.M2011136. The invention also discloses application of the efficient phosphate-solubilizing penicillium pinophilum of laxus malrel rhizosphere to accelerating the growth of the laxus malrel. The bacterial strain disclosed by the invention has stronger dissolving effects on difficultly-soluble phosphates, such as tricalcium phosphate, ferric phosphate, aluminum phosphate, Jinping powdered rock phosphate in Jiangsu and hydroxyapatite; and the bacterial strain is prepared into microbial inoculums which are respectively inoculated into cuttage seedlings and seedling plants of the laxus malrel and the result shows that the bacterial strain can obviously accelerate the growth and development of the cuttage seedlings and seedling plants of the laxus malrel. Therefore, according to the invention, good bacterial strain resources can be supplied to develop a special microorganism fertilizer of the laxus malrel in the future.

The invention discloses a masson pine rhizosphere solubilizing phosphorus fungus aspergillus awamori which is aspergillus awamori JP-NJ1 and application thereof. The aspergillus awamori JP-NJ1 is collected in the China Center for Type Culture Collection (CCTCC) on May 14, 2012 with the collection number of CCTCC No: M2012166. The aspergillus awamori JP-NJ1 disclosed by the invention has strong capacity of dissolving the insoluble phosphate which is the calcium phosphate. The room-temperature pot culture experiments prove that the aspergillus awamori JP-NJ1 can promote the growth of the massonpine seedlings significantly. Therefore, the aspergillus awamori provides excellent strain resources for the development of the masson pine growth promoting bacteria and has good application and development prospects.

The invention belongs to the technical field of Escherichia coli, and particularly relates to a wide-lysis-spectrum Escherichia coli phage and a composition, a kit and application thereof. The phage is Escherichia coli phage CL1 which is collected under the number of CCTCC M 2018936. The phage is nonhazardous to normal microbial flora, resistant to high temperature and high in stability and has good application and development prospect, DNA of the phage cannot code virulence genes, and an excellent strain resource is provided for developing novel antibacterial preparations.

The invention discloses enterobacter ludwigii PN6 and application thereof. The classification name of the strain is Enterobacter ludwigii with a strain number of PN6, the strain is collected in the China Center for Type Culture Collection and has a collection number of CCTCC NO: M 2019881. The strain PN6 screened from a Phyllostachys edulis root system has an obvious anti-bacteria function for a pathogenic bacterium, i.e., C .phyllostachydis, of the shoot blight of Phyllostachys edulis, a fermentation liquor inhibition rate of the strain PN6 can be 49.28%, and in addition, the strain PN6 has acapacity of efficiently dissolving phosphorus and secreting IAA (indole-3-acetic acid). The strain PN6 disclosed by the invention has an acceleration function for the growth of Phyllostachys edulis,an experiment data displays that a liquid fungicide prepared from the PN6 has an obvious growth promotion function for growth indexes, including the ground diameter, the seedling height, the chlorophyll and the like of the Phyllostachys edulis, in addition, the stress resistance of the Phyllostachys edulis can be increased, and an occurrence probability of the shoot blight of Phyllostachys edulisis reduced. Therefore, the invention provides an excellent strain resource for developing an environmentally-friendly special biofertilizer for the Phyllostachys edulis.

The invention discloses a bacillus fusiformis for promoting rhizosphere growth of blueberries. The bacterial strain is named as bacillus fusiformis (Bacillus fusiformis) L13 in class, and preserved at the general microbiological center of the China Committee for Culture Collection of Microorganisms on December 31, 2012; and the preservation number is CGMCC No.7068. The invention also provides application of the bacillus fusiformis for promoting rhizosphere growth of the blueberries. The bacillus fusiformis for promoting rhizosphere growth of the blueberries disclosed by the invention is screened from rhizosphere soil of the blueberries, adapts with a special rhizosphere soil environment of the blueberries, and can promote growth of the blueberries.

The invention relates to the field of biology, in particular to novel staphylococcus aureus bacteriophages as well as a composition and application thereof, and provides four staphylococcus aureus bacteriophages including: podoviridae BP-13 with the preservation number of CCTCC NO: M 2015142; staphylococcus aureus BP-13A with the preservation number of CCTCC M2016535; podoviridae BP-14 with the preservation number of CCTCC NO: M 2015143; and podoviridae AHJD-like phage BP-39 with the preservation number of CCTCC NO: M 2015144. The four bacteriophages are strict virulent bacteriophages, can effectively kill various staphylococcus aureus such as MRSA, MSSA, BORSA and the like, have a wide host range, but have no toxic effect on normal microbial flora, and the DNA of the bacteriophages cannot encode virulence genes. Therefore, the bacillus subtilis can be used for preventing or treating human and livestock bacterial infection caused by the staphylococcus aureus, provides an excellent strain resource for developing a novel antibacterial preparation, and has a good application and development prospect. Besides, the host range and the antibacterial efficiency of the cocktail mixture disclosed by the invention are further greatly improved.

The invention discloses a strain of bacillus cereus which is preserved in China General Microbiology Center of Committee for Culture Collection of Microorganisms since 31st December, 2014, and the preservation number is CGMCC No. 7069. The bacillus cereus L90 provided by the invention is capable of improving the drought resistant capability of juglans regia. Experiment research shows that after the bacillus cereus provided by the invention is inoculated to the surround of juglans regia roots and juglans regia is stressed by drought for a certain time, juglans regia is substantially improved in bacterial community structure diversity in rhizosphere soil compared with juglans regia inoculated with common growth-promoting bacteria, the leaf relative water content and the water utilization efficiency are improved, and the drought resistance of the plant is enhanced. Therefore, the bacillus cereus provided by the invention or a bacterium preparation by taking bacillus cereus as a raw material can be used as an inoculation agent and applied to drought environment, and the bacillus cereus provided by the invention helps to provides an excellent bacterial strain resource for improving the drought resistance of juglans regia.

The invention discloses salinicola zeshunii JP11 with salt-tolerant and growth-promoting functions. The strain is deposited in China General Microbiological Culture Collection Center on September 14, 2021 and has the deposit number of CGMCC No.23407. The invention also discloses use of the salinicola zeshunii in relieving an inhibition effect of salt stress on rice growth and a liquid culture medium suitable for fermentation by the strain. Experiments confirm that the salinicola zeshunii JP11 has various growth promoting characteristics such as dissolving potassium and dissolving and solubilizing phosphorus, high salt tolerance and a wide salt tolerance spectrum. A bacterial solution prepared by using the JP11 can improve salt tolerance and growth promoting of rice, accelerates a seedling growth and increases biomass under salt stress, and thus promotes rice germination and seedling growth. Meanwhile, the provided JP11 can increase the content of soluble potassium in the soil to 25.4% and the content of soluble phosphorus in the soil to 45.9%, thus effectively improves a utilization rate of the soil and provides an excellent strain resource for saline-alkali farmland soil remediation.

The invention discloses Aspergillus welwitschiae and application thereof. The preservation number of the Aspergillus welwitschiae JX-S3 disclosed by the invention is CCTCC (China Center For Type Culture Collection) NO: M 2020217; the Aspergillus welwitschiae is obtained by screening from rhizosphere soil of cunninghamia lanceolata and has and has efficient phosphate solubilizing capacity; the Aspergillus welwitschiae has better dissolving capability on five kinds of insoluble phosphates including calcium phosphate (Ca3(PO4)2), ferric phosphate (FePO4), calcium hydrophosphate (CaHPO4), aluminumphosphate (AlPO4) and calcium phytate (C6H6Ca6O24P6); and a liquid inoculant prepared from the strain has a remarkable growth promoting effect on the ground diameter and seedling height of the cunninghamia lanceolata. Therefore, an excellent strain resource is provided for developing an environment-friendly biological special fertilizer for the cunninghamia lanceolata.

The invention relates to the field of biology, in particular to novel staphylococcus aureus bacteriophages as well as a composition and application thereof, and provides four staphylococcus aureus bacteriophages, including podoviridae BP-13 with the preservation number of CCTCC NO: M 2015142. staphylococcus aureus BP-13A with the preservation number of M 2016535; podoviridae BP-14 with the preservation number of NO: M 2015143; and podoviridae AHJD-like phage BP-39 with the preservation number of NO: M 2015144. The four bacteriophages are strict virulent bacteriophages, can effectively kill various staphylococcus aureus such as MRSA, MSSA, BORSA and the like, have a wide host range, but have no toxic effect on normal microbial flora, and the DNA of the bacteriophages cannot encode virulence genes. Therefore, the bacillus subtilis can be used for preventing or treating human and livestock bacterial infection caused by the staphylococcus aureus, provides an excellent strain resource for developing a novel antibacterial preparation, and has a good application and development prospect. In addition, the host range and the antibacterial efficiency of the cocktail mixture disclosed by the invention are further greatly improved.

The invention discloses a strain of Serratia marcescens, which is classified as Serratia sp. and has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on January 11, 2016, and the preservation number is CGMCC NO.11987. The Serratia marcescens of the present invention has an obvious inhibitory effect on A549 non-small cell lung cancer and S180 ascites tumor, and the curative effect is very outstanding. Therefore, the Serratia marcescens can be used as a raw material to prepare a drug for inhibiting tumors, For example: culture the bacteria to obtain a culture, and add or not add other drugs, auxiliary materials, etc. to process and prepare the bacterial agent. The invention provides an excellent strain resource for studying the tumor suppressing mechanism, application, drug development and the like of the Serratia bacterium.

The invention discloses acinetobacter calcoaceticus for promoting rhizosphere growth of walnuts. The acinetobacter calcoaceticus is named acinetobacter calcoaceticus (Acinetobacter calcoaceticus) X128 in class, and preserved at the general microbiological center of the China Committee for Culture Collection of Microorganisms on December 31, 2012; and the preservation number is CGMCC No.7071. The invention also provides application of the acinetobacter calcoaceticus for promoting rhizosphere growth of the walnuts. The acinetobacter calcoaceticus for promoting rhizosphere growth of the walnuts is evenly fed to the periphery of the walnut root in a pouring manner; root growth can be obviously facilitated; the quantity of lateral roots is improved; and growth of the bottom diameter of the walnut and the height of seedling is facilitated. In contrast, the difference reaches a significant level. Therefore, excellent strain resources are provided for developing a special microbial fertilizer for the walnut in the future.

The invention discloses a burkholderia lata strain PN1 and application thereof. The classification name of the strain is Burkholderia lata, the strain number is PN1, the strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO:M 2019880. The strain PN1 screened out from phyllostachys edulis root systems has a significant inhibiting effect on phyllostachys edulis shoot blight pathogens ceratosphaeria phyllostachydis, a bacteriostasis rate of fermentation broth of the strain PN1 screened out from the phyllostachys edulis root systems can reach58.82%, and the strain PN1 screened out from the phyllostachys edulis root systems has efficient capability of phosphate solubilizing and IAA secretion; and the phyllostachys edulis endophytic bacteria PN1 can significantly promote growth of phyllostachys edulis seedlings, and test data shows that a liquid bacterial agent produced from the strain PN1 significantly increases growth indexes of the ground diameter, the seedling height, chlorophyll and the like of phyllostachys edulis. Therefore, excellent strain resources are provided for development of an environment-friendly bio-fertilizer special for the phyllostachys edulis.

The invention provides phosphate solubilizing bacterium rhizosphere fungus and application thereof to promotion of growth of blueberries and belongs to the technical field of biology. A strain is WR1 and has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.13884; when the strain is inoculated back to a blueberry tissue culture seedling and a blueberry seedling, the strain has a remarkable growth promotion effect. Therefore, the bacterium rhizosphere fungus WR1 can provide a good strain resource for development of microorganism bacterium fertilizer and has a good application and development prospect.

The invention discloses a kind of Burkholderia pyrrocinia, which is classified as Burkholderia pyrrocinia (Burkholderia pyrrocinia), and the strain number is LWK2, which has been preserved in the China Type Culture Collection Center CCTCC, and the preservation number is : CCTCC NO: M 2016007, date of deposit: January 5, 2016. The invention also discloses the application of the above-mentioned Burkholderia pyrrole in promoting the growth of the japonica. The LWK2 strain has strong potassium-decomposing ability and auxin secretion ability; it can obviously promote the germination of cucumber seeds; the LWK2 bacterial agent was inoculated with the seedlings of Lianxiang tree, and the results showed that the bacterial agent can obviously promote the growth of Lianxiang tree seedlings. At the same time, the strain has a certain antagonistic effect on plant pathogens such as rice blast fungus, ascosporium chrysogenum, Fusarium oxysporum, rice sheath blight fungus and rhizoctonia.

The invention relates to pear rhizosphere growth promoting acinetobacter junii Lzh-X15 and application thereof. The preservation number of the acinetobacter junii Lzh-X15 is CGMCC No.13490, and the preservation date is September 23rd, 2016. The invention further relates to application of the acinetobacter junii Lzh-X15, fermentation liquor and microbial fertilizers containing the acinetobacter junii Lzh-X15 in the aspect of preventing and treating pear root rot. A microbial fertilizer prepared by the acinetobacter junii Lzh-X15 disclosed by the invention can obviously reduce the probability that pears are infected by root rot, and furthermore, pear yield and quality are improved.

The invention discloses bacillus mycoides FXY-WH10. The bacillus mycoides has been collected in the China Center for Type Culture Collection (CCTCC), with collection number being CCTCC NO:M 2012263 and collection date being July 1, 2012. The invention also discloses application of the bacillus mycoides for promoting growth of liquidambar formosana. The FXY-WH10 strains have stronger lecithin degradation capabilities. The FXY-WH10 strains are inoculated to the liquidambar formosana seedlings and the results show that the strains can obviously promote growth of the liquidambar formosana seedlings. Therefore, the invention provides excellent strain resources for developing liquidambar formosana phosphorus bacterial fertilizers.

The invention discloses a Pseudomonas putida, which is classified as Pseudomonas putida and has a strain number of LWPZF. It has been preserved in the China Center for Type Culture Collection CCTCC, and the preservation number is CCTCC NO: M 2016008. The deposit date is January 5, 2016. The invention also discloses the application of the above-mentioned Pseudomonas putida in promoting the growth of the japonica. The LWPZF strain has a strong ability to decompose inorganic phosphorus and auxin secretion; it can significantly promote the germination of cucumber seeds; the LWPZF bacterial agent was inoculated with the seedlings of Lianxiang tree, and the results showed that the bacterial agent can significantly promote the growth of Lianxiang tree seedlings. At the same time, the strain has a certain antagonistic effect on plant pathogens such as Fusarium oxysporum, rice sheath blight, Rhizoctonia and rice blast fungus. Therefore, the present invention provides excellent bacterial strain resources for the development of the special biological fertilizer for the syringa japonica.

The invention discloses an endophytic Bacillus amyloliquefaciens JL‑B05 and its application, belonging to the technical field of microorganisms. Endophytic Bacillus amyloliquefaciens JL‑B05 taxonomically named Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) JL‑B05, which was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms on November 22, 2019, with the deposit number: CGMCC No.18998. The bacterial strain can effectively inhibit the mycelium growth and spore germination of edible fungus pathogenic bacteria. The volatile substances of the strain have a strong inhibitory effect on the growth of pathogenic mycelia. The bacterial strain has great application value in the prevention and treatment of edible fungus pathogenic bacteria.

N The invention discloses aspergillus violaceofuscus and an application thereof. The preservation number of the aspergillus violaceofuscus JX-S1 disclosed by the invention is CCTCC NO:M 2020216. The aspergillus violaceofuscus JX-S1 is screened from rhizosphere soil of a cunninghamia lanceolata man-made forest and has efficient phosphate solubilizing capacity; under different initial pH values, theJX-S1 strain has good growth and adaptability, and has a strong dissolving effect on five insoluble phosphates: calcium phosphate, iron phosphate, aluminum phosphate, calcium hydrophosphate and calcium phytate; a liquid inoculant prepared from the strain has a remarkable growth promoting effect on the ground diameter and seedling height of cunninghamia lanceolata. Therefore, an excellent strain resource is provided for developing an environment-friendly cunninghamia lanceolata biological special fertilizer.

The invention discloses a serratia marcescens FXW-NJ2D, which is classified and named as serratia marcescens subsp marcescens. The serratia marcescens is preserved in China Centre for Type Culture Collection with the preservation number of CCTCC M 2012261. The invention further discloses an application of the serratia marcescens for promoting the growth of Chinese sweetgum. Under the condition ofliquid shake-flask culture, the bacterial strain in the invention has a strong dissolving effect on insoluble phosphates such as tricalcium phosphate, iron phosphate, aluminium phosphate and hydroxyapatite; the bacterial stain is prepared into a fungicide, and the fungicide is inoculated in a Chinese sweetgum seedling; the results indicate that the fungicide can obviously promote the growth of Chinese sweetgum; and therefore, an excellent bacterial strain resource is provided for developing a special microbial fertilizer for Chinese sweetgum in the future.

The invention discloses a tea tree growth-promoting rhizobacterium of which the classification designation is Serratia marcescens. The strain number is JW-CZ2. The Serratia marcescens is collected by China Center for Type Culture Collection, the collection number is CCTCC M 2015095, and the collection date is March 10th, 2015. The invention also discloses application of the Serratia marcescens in promoting tea tree growth. The JW-CZ2 strain has high solubility for insoluble phosphates, such as tricalcium phosphate, magnesium phosphate and iron phosphate. The JW-CZ2 strain has IAA (indoleacetic acid) secretion capacity and ACC deaminase producing capacity. When a tea tree seedling plant is inoculated in the JW-CZ2 microbial inoculant, the microbial inoculant can obviously promote the growth of the tea tree seedling. Therefore, the tea tree growth-promoting rhizobacterium Serratia marcescens can provide an excellent strain resource for future development of special microbial fertilizers for tea trees.

The invention belongs to the field of biotechnology, and in particular relates to a broad-spectrum colibacillus phage and its application. The Escherichia coli phage WXLECP008 (Escherichia coli phage, WXLECP008) provided by the present invention is a virulent phage, which has tolerance at pH 4 to pH 10 and at -80°C to 65°C, cultivated for 8 hours under the condition of MOI=0.1, and its titer is 3.5×10 10 pfu / mL. The Escherichia coli phage provided by the invention is a broad-spectrum phage with a wide cleavage range, has high titer and high safety, can reproduce in large quantities in non-pathogenic host bacteria, and can be used for large-scale fermentation production. The phage described in the invention can provide excellent bacterial strain resources for the development of phage therapy, has good application and development prospects, and is suitable for popularization and application.

The invention discloses a strain of salt-tolerant rhizosphere growth-promoting Enterobacter cloacae and its application. The strain is named Enterobacter cloacae (Enterobacter cloacae) TIA062, and has been preserved in China's Microbiological Culture Collection Management on January 7, 2016. Committee General Microbiology Center, deposit number is CGMCC No.11974. The salt-tolerant rhizosphere growth-promoting enterobacter cloacae of the invention can improve the salt-alkali tolerance of white wax. The salt-tolerant rhizosphere growth-promoting Enterobacter cloacae of the present invention is inoculated around the root system of white wax, and after a certain period of salt stress, it can increase the water content of the root system of white wax and promote the growth of white wax compared with no inoculation agent. Therefore, the present invention The invention can be used as an inoculant in a saline environment, and provides excellent strain resources for improving the saline-alkali resistance of white wax.

The present invention relates to a Gorgon fruit rhizosphere growth-promoting bacterium, which is classified as Baclicus lincheniformis Q67, preserved in the China Center for Typical Culture Collection, and the preservation number is CCTCC NO: M2014652. The Gorgon fruit rhizosphere growth-promoting bacteria of the present invention Bacteria Q67 can significantly promote the growth and development of Gorgon seedlings, and increase the content of available nitrogen in the soil environment where Gorgon grows. The present invention also relates to the application of the above-mentioned rhizosphere growth-promoting bacteria of Gorgon fruit in the preparation of bacterial agent, and the result shows that the bacterial agent can obviously improve the germination rate of Gorgon fruit seed.

The invention discloses a strain of Burkholderia latta PN1 and its application. The classification of the strain is named Burkholderialata, the strain number is PN1, and it is preserved in the China Center for Type Culture Collection with the preservation number: CCTCC NO: M 2019880. The bacterial strain PN1 screened from the root system of moso bamboo in the present invention has a significant antibacterial effect on the pathogen of moso bamboo shoot die-back, Phyllostachys coccus, the antibacterial rate of its fermentation broth can reach 58.82%, and it has high-efficiency phosphorus-solubilizing and IAA-secreting abilities; The endophytic bacterium PN1 of moso bamboo of the present invention can significantly promote the growth of moso bamboo seedlings, and test data show that the liquid bacterial agent prepared by the strain PN1 can significantly improve growth indexes such as ground diameter, seedling height and chlorophyll of moso bamboo. Therefore, the present invention provides excellent bacterial strain resources for developing environment-friendly moso bamboo biological special fertilizers.

A phosphate-solubilizing mycorrhizal fungus and its application in promoting blueberry growth belong to the field of biotechnology. The strain is WR1, and its preservation number is CGMCC No.13884; significant growth-promoting effect. Therefore, the mycorrhizal fungus WR1 of the present invention can provide excellent strain resources for the development of microbial fertilizers, and has good application and development prospects.