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Masson pine rhizosphere solubilizing phosphorus fungus aspergillus awamori and application thereof

A technology of Aspergillus awamori and phosphorus-solubilizing fungus, applied in the field of microorganisms, can solve the problems of less research on phosphorus-solubilizing fungi, and achieve the effects of excellent strain resources, growth promotion, and good application and development prospects

Active Publication Date: 2012-10-17
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phosphorus solubilizing fungi have been less studied

Method used

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  • Masson pine rhizosphere solubilizing phosphorus fungus aspergillus awamori and application thereof
  • Masson pine rhizosphere solubilizing phosphorus fungus aspergillus awamori and application thereof
  • Masson pine rhizosphere solubilizing phosphorus fungus aspergillus awamori and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The rhizosphere phosphorus-solubilizing fungus Aspergillus awamori (Aspergillus awamori) JP-NJ1 was screened from the rhizosphere soil of 30-40-year-old Pinus massoniana in Nanjing Forestry University. Such as figure 1 As shown, the colony grows rapidly on the PDA medium, and the diameter of the colony is about 65mm after 7 days of cultivation at 25°C. The colony is black, has radial grooves, and has a velvety texture; the reverse side of the colony is slightly light yellow. The conidiophores are mostly radial when they are young, with a diameter of 70-150um, and loose when they are old, with a diameter of about 300-500um; conidia are produced in large numbers, and they are densest in the middle of the colony and appear dark brown. Conidiophores occur in the stroma, about 600-800um×10um, pale brown when old, and fertile on the entire surface; the spore-producing structure is double-layered, the conidia are spherical or nearly spherical, 3.5-5um in diameter, and the wall...

Embodiment 2

[0024] Inoculate Aspergillus awamori JP-NJ1 on the NBRIP plate, culture at 25°C, observe whether there is still a lysing zone after 7-10 days, measure the diameter of the colony (D) and lysing zone (d), and calculate the diameter of the lysing zone and the diameter of the colony The ratio (d / D) of the phosphate-solubilizing fungi can be preliminarily estimated. Generally, the larger the ratio, the stronger the phosphorus-solubilizing ability of the fungus. According to Table 1, those whose phosphorus dissolving ability is "++" are considered to have stronger phosphorus dissolving ability. The d / D value of Aspergillus awamori JP-NJ1 was measured according to the above method, and the measured ratio was 1.44, indicating that the JP-NJ1 strain has a strong phosphorus-solubilizing ability.

[0025] Table 1 Grading standard of phosphorus-solubilizing ability of phosphorus-solubilizing fungi

[0026] d / D

Embodiment 3

[0028] Inoculate the Aspergillus awamori JP-NJ1 strain on the PDA plate, cultivate it for 10 days, and after activation, take the bacterial block (60mm in diameter) from the plate and inoculate it into a 100mL Erlenmeyer flask containing 50mL NBRIP liquid medium. NBRIP medium is the control (CK), each bottle is inoculated with 6 bacterial blocks of the same volume, and each strain to be tested is set up in triplicate, 25°C, 200r min -1 Shaking culture, after 7 days, centrifuge the fermentation broth for 10min (4°C, 10000r min -1 ), using the molybdenum-antimony anti-colorimetric method, that is, take 100uL of the supernatant obtained by the above centrifugation and add it to a 50mL volumetric flask, then add about 25mL of deionized water, and then add 1~2 drops of 2,4-dinitro Phenol indicator, add 5mL molybdenum antimony anti-color agent, add deionized water to make up to volume, mix well, after standing for 0.5h, measure the OD value. And calculate the available phosphorus c...

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Abstract

The invention discloses a masson pine rhizosphere solubilizing phosphorus fungus aspergillus awamori which is aspergillus awamori JP-NJ1 and application thereof. The aspergillus awamori JP-NJ1 is collected in the China Center for Type Culture Collection (CCTCC) on May 14, 2012 with the collection number of CCTCC No: M2012166. The aspergillus awamori JP-NJ1 disclosed by the invention has strong capacity of dissolving the insoluble phosphate which is the calcium phosphate. The room-temperature pot culture experiments prove that the aspergillus awamori JP-NJ1 can promote the growth of the massonpine seedlings significantly. Therefore, the aspergillus awamori provides excellent strain resources for the development of the masson pine growth promoting bacteria and has good application and development prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to Aspergillus awamori, a rhizosphere phosphorus-solubilizing fungus of Pinus massoniana and its application. Background technique [0002] Phosphate-solubilizing microorganisms (PSMs) are a special functional group of microorganisms that can convert insoluble phosphorus into soluble phosphorus that plants can absorb and utilize in soil, including phosphorus-solubilizing fungi, phosphorus-solubilizing bacteria, and phosphorus-solubilizing actinomycetes. Wait. Although the number and types of Solubilizing phosphorus fungus are less than those of Phosphorus-dissolving bacteria, studies have shown that the Phosphorus-dissolving ability of Phosphorus-dissolving fungi is generally stronger than that of bacteria, sometimes even dozens of times that of bacteria, and their genetic traits are more Stablize. So far, the phosphorus-solubilizing microorganisms found are mai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00C05G3/00C12R1/665
Inventor 吴小芹魏伟叶建仁
Owner NANJING FORESTRY UNIV
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