The invention discloses a method for improving the
mass spectrum identification number of a
protein and / or
peptide fragment group by using a
polystyrene material. The method comprises the following steps of: adding a binding
buffer solution into a sample to be detected, and adding the
polystyrene material to obtain a suspension; incubating the suspension, performing high-speed
centrifugation, removing supernate, and retaining a precipitate I; re-suspending the precipitate I by using a cleaning
buffer solution to obtain a re-suspension, centrifuging the re-suspension at a high speed, removing a supernatant, retaining a precipitate II, and repeating the step for several times; and preparing the precipitate II into a
proteome /
peptide fragment group sample, and carrying out
mass spectrometric detection. The
polystyrene material and the corresponding buffer solutions are adopted, different kinds of serum /
plasma proteins and
peptide fragments can be effectively adsorbed, selective enrichment of low-abundance proteins and peptide fragments in serum /
plasma is achieved, interference of high-abundance proteins in the serum /
plasma on identification of the low-abundance proteins during
mass spectrum identification is effectively avoided. The method can be used for identifying various
protein spectrums.