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Selective enrichment method of anthocyanin compounds in Lycium ruthenicum

A technology of Lycium barbarum and anthocyanins, applied in the field of analytical chemistry, can solve the problems of poor selectivity, time-consuming, low efficiency of anthocyanin components, etc.

Active Publication Date: 2016-12-07
TAIZHOU GUOKEHUAWU BIOMEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the similar hydrophobicity of anthocyanin compounds and non-anthocyanin impurities in the sample, the conventional reverse phase method is inefficient, time-consuming, and has poor selectivity for the preparation of anthocyanin fractions

Method used

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  • Selective enrichment method of anthocyanin compounds in Lycium ruthenicum
  • Selective enrichment method of anthocyanin compounds in Lycium ruthenicum

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Embodiment 1

[0022] 1) The 70% (v / v) ethanol-formic acid water (pH 2) extract of Lycium barbarum is first separated by a weak polar macroporous resin, and the washing conditions are as follows: separation material: AB-B macroporous resin; washing 1: Separation conditions: the resin is AB-8 macroporous resin (50×520mm); the washing conditions are: washing 1: pure water; washing 2: 50% (v / v) ethanol-water, and recovering the solvent to obtain Lycium barbarum For the above samples, XCharge SCX strong cation exchange solid-phase extraction column was used to selectively enrich anthocyanin compounds, and then desalted by AB-8 macroporous resin to prepare anthocyanin fraction.

[0023] 2) For the Lycium barbarum samples, XCharge SCX strong cation-exchange solid-phase extraction column was used to selectively enrich anthocyanin compounds. ); the rinse conditions are: rinse 1: 5% (v / v) acetonitrile-formic acid water 60mL (acid water volume concentration is 0.1%); rinse 2: 30% (v / v) acetonitrile-wa...

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Abstract

The invention discloses a selective enrichment method of anthocyanin compounds in Lycium ruthenicum. The method concretely comprises the following steps: anthocyanin compounds are eluted at a condition with low pH, in order to guarantying stability of the compounds; an acidified alcohol extract product of Lycium ruthenicum is separated by macroporous resin with weak polarity, in order to obtain a Lycium ruthenicum sample; the obtained Lycium ruthenicum sample passes a strong cation exchange solid phase extraction column of the Lycium ruthenicum sample is obtained, and an anthocyanin compound crude component in the sample is enriched; the obtained anthocyanin compound crude component is desalinated by macroporous resin with weak polarity in order to prepare a refined component. The method can be used for realizing selective enrichment of anthocyanin compounds in a complex system, solving the problem that the compound and impurities flow out together on an anti-phase material, and realizing separation of the kind of the compounds. A new method for selective enrichment of anthocyanin compound is established, and a technical support for in-depth research of biological activity of the compounds is provided.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a selective enrichment method for anthocyanin compounds in Lycium barbarum, in particular to a selective enrichment method using a strong cation exchange solid-phase extraction column under low pH conditions A new method for anthocyanin compounds, which shows a better separation effect on the enrichment of anthocyanin compounds in complex systems. Background technique [0002] Anthocyanin is a natural water-soluble pigment that widely exists in plants in nature. Anthocyanins belong to flavonoids. Due to its unique functionality, it has been used to scavenge free radicals in the body, proliferate lutein, anti-tumor, anti-cancer, anti-inflammatory, inhibit lipid peroxidation and platelet aggregation, prevent diabetes, lose weight, protect eyesight, etc. (Hou, D.X.(2003). Potential mechanisms of cancer chemoprevention by anthocyanins. Current Molecular Medicine, 3(2...

Claims

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Application Information

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IPC IPC(8): G01N1/40
Inventor 梁鑫淼金红利刘艳芳郭志谋张秀莉薛兴亚
Owner TAIZHOU GUOKEHUAWU BIOMEDICAL TECH CO LTD
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