Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for selective enrichment of dicaffeoylspermidine compounds in Lycium barbarum

A technology of caffeoyl spermidine and wolfberry fruit, which is applied in chemical instruments and methods, carboxylic acid amide separation/purification, separation methods, etc., can solve the problems of low efficiency, poor selectivity, time-consuming and the like

Active Publication Date: 2022-03-08
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the similar hydrophobicity of dicaffeoylspermide compounds and non-dicaffeoylspermide impurities in the sample, the conventional reversed-phase method is inefficient, time-consuming, and selective. poor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for selective enrichment of dicaffeoylspermidine compounds in Lycium barbarum
  • A method for selective enrichment of dicaffeoylspermidine compounds in Lycium barbarum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1) Use 90% (v / v) ethanol-water solution as the extraction solvent (solid-to-liquid ratio: 1:10) for 5 g of Lycium barbarum, ultrasonically extract for 3 hours, suction filter, and centrifuge to obtain Lycium barbarum extract.

[0024] 2) A strong cation-exchange solid-phase extraction column was used to selectively enrich the crude component of dicaffeoylspermidine for the extract of wolfberry fruit. The solid-phase extraction conditions: the solid-phase extraction column was a strong cation solid-phase extraction column (1g / 6mL , 60 μm); sample volume: 4ml (Fruit medlar extract); washing conditions: washing 1: 90% (v / v) methanol-water solution 5mL; washing 2: 30% (v / v) acetonitrile -Water 5mL, rinse 2 solution with 1.0M sodium dihydrogen phosphate to adjust the pH value to 2.5, collect the eluate, concentrate under reduced pressure until the organic solvent is completely removed, and obtain the crude fraction of dicaffeamide.

[0025] 3) Desalting the crude fraction of...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a selective enrichment method for dicaffeoylspermide compounds in Lycium barbarum. Specifically, the dicaffeoylspermidine compounds are eluted under low pH conditions to ensure that such compounds The stability of the compound, the ethanol extract of Lycium barbarum was separated by strong cation exchange solid phase extraction to obtain the crude dicaffeoylspermide component, and then the obtained dicaffeoylspermide component was subjected to reversed-phase solid-phase extraction. Extraction column is desalted to prepare refined components; this method can realize the selective enrichment of dicaffeoylspermidine compounds in complex systems, solve the problem of "co-elution" of such compounds and impurities on reversed-phase materials, and realize Class separation of this class of compounds. Therefore, the present invention establishes a new method for the selective enrichment of dicaffeoylspermide compounds, providing technical support for the research and development of new drugs of such compounds.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a selective enrichment method for dicaffeoylspermidine compounds in Lycium barbarum, in particular to a selective enrichment method using a strong cation exchange solid-phase extraction column under low pH conditions. A new method for the enrichment of dicaffeoylspermidine compounds, which shows a better separation effect on the enrichment of dicaffeoylspermide compounds in complex systems. Background technique [0002] Dicaffeoylspermidine is a plant secondary metabolite present in Solanaceae plants. Dicaffeoylspermide belongs to amides. According to literature reports, this type of chemical composition has various pharmacological activities such as scavenging free radicals in the body, anti-aging, neuroprotection, anti-tumor, anti-cancer, anti-inflammation, and prevention of Alzheimer's disease. (Li Y Y, Di R, Baibado J T, etal. Identification of kukoamines as ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07C231/24C07C235/34B01D15/18B01D15/36B01D15/32
Inventor 梁鑫淼艾散江·艾海提金红利刘艳芳郭志谋
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com