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Ultrathin lamellar functionalized molybdenum disulfide nanocomposite material and its application in glycopeptide enrichment

A technology of molybdenum disulfide and composite materials, which is applied in the field of materials, can solve the problems of inability to analyze glycoproteins and glycopeptides, lack of versatility, difficulty in releasing sugar chain structures, etc., and achieve simple synthesis methods, increase specific surface area, Overcoming the effect of easy agglomeration

Active Publication Date: 2018-02-23
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, lectin affinity chromatography is currently the most widely used enrichment method for glycoproteins and glycopeptides, but it only has a very good effect on glycopeptides / glycoproteins of specific glycoforms and lacks versatility
The hydrazine chemistry method is based on the oxidation of the adjacent dihydroxyl groups on the sugar chains to aldehyde groups using sodium periodate, and then the use of acyl-functionalized materials to covalently bind the aldehyde groups to achieve the enrichment of glycopeptides / glycoproteins, so It has high enrichment selectivity, but the formation of covalent bonds makes it difficult to release the sugar chain structure, and it is impossible to analyze the glycoforms of the enriched glycoproteins and glycopeptides

Method used

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  • Ultrathin lamellar functionalized molybdenum disulfide nanocomposite material and its application in glycopeptide enrichment
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  • Ultrathin lamellar functionalized molybdenum disulfide nanocomposite material and its application in glycopeptide enrichment

Examples

Experimental program
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Effect test

Embodiment 1

[0068] Example 1. The enrichment and detection of glycopeptides by the functionalized molybdenum disulfide-nanogold composite material based on the principle of hydrophilic enrichment comprises the following steps:

[0069] 1. Preparation of molybdenum disulfide-nano gold:

[0070] (1) Ultrasonic dispersion of 10 mg of molybdenum disulfide powder in 1 mL of n-hexane.

[0071] (2) 3 mg of gold tetrachloride trihydrate and 100 μL of oleylamine were dissolved in 1.5 mL of n-hexane.

[0072] (3) Ultrasonic dispersion after mixing the product obtained in step (1) and the product obtained in step (2).

[0073] (4) Add 150 μL of triisopropylsilane (TIPS) to the product mixture obtained in step (3), mix well and let stand for 4 hours.

[0074] (5) Add absolute ethanol to the product obtained in step (4), mix well and centrifuge at 4,500 g for 15 minutes.

[0075] (6) Take the precipitate obtained in step (5), add n-hexane to dissolve the precipitate, then add absolute ethanol, mix ...

Embodiment 2

[0090] Example 2. Sensitivity detection of glycopeptide enrichment by functionalized molybdenum disulfide-nanogold composite material based on hydrophilic enrichment principle, including the following steps:

[0091] 1. Enrichment of glycopeptides:

[0092] (11) Weigh 1 mg of the functionalized molybdenum disulfide-nano noble metal composite material obtained in Example 1, shake and disperse it in 1 mL of enrichment buffer (acetonitrile / water / trifluoroacetic acid, 86:13.9:0.1, v / v / v) In this method, take 20 μL of the above suspension, centrifuge and remove the sediment from the lower layer, and wash 3 times with enrichment buffer.

[0093] (12) Take 100 μL enrichment buffer (acetonitrile / water / trifluoroacetic acid, 86:13.9:0.1, v / v / v), wherein the contents of IgG trypsin-digested peptides in Group 1, Group 2 and Group 3 were 6.7pmol, 20fmol and 10fmol respectively.

[0094] (13) After suspending the precipitate obtained in step (11) in the solutions of group 1, group 2 and ...

Embodiment 3

[0099]Example 3. Selective and specific detection of glycopeptide enrichment by functionalized molybdenum disulfide-nanogold composite material based on the principle of hydrophilic enrichment, including the following steps:

[0100] 1. Enrichment of glycopeptides:

[0101] (11) Weigh 1 mg of the functionalized molybdenum disulfide-nano noble metal composite material obtained in Example 1, shake and disperse it in 1 mL of enrichment buffer (acetonitrile / water / trifluoroacetic acid, 86:13.9:0.1, v / v / v) In this method, take 20 μL of the above suspension, centrifuge and remove the sediment from the lower layer, and wash 3 times with enrichment buffer.

[0102] (12) Take 100 μL enrichment buffer (acetonitrile / water / trifluoroacetic acid, 86:13.9:0.1, v / v / v), wherein the mass ratios of the mixtures each containing 2 μg of IgG and BSA digestion products in Group 1 and Group 2 were 1:200 and 1:500, respectively.

[0103] (13) After suspending the precipitate obtained in step (11) in ...

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Abstract

The invention discloses an ultrathin lamellar functionalized molybdenum disulfide nanocomposite material and its application in glycopeptide enrichment. A glycopeptide enrichment material utilizes anultrathin two-dimensional lamellar structure molybdenum disulfide nano-material as a matrix. After surface modification with nano-gold, through a simple and stable Au-S bond chemical reaction, a mercapto-containing glycopeptide enrichment functional group is loaded. An experimental result shows that the functionalized molybdenum disulfide-nano-noble metal composite material and glycopeptides produce hydrophobic interaction or through a reversible reaction of the boronic acid group supported on the surface of the material and the sugar chain of the glycopeptides, the functional material realizes the highly selective enrichment of glycopeptides. The functionalized molybdenum disulfide-nano-noble metal composite material is prepared through simple processes under mild reaction conditions.

Description

technical field [0001] The invention belongs to the field of materials, and relates to an ultra-thin lamellar functionalized molybdenum disulfide nanocomposite material and its application in glycopeptide enrichment. Background technique [0002] Glycosylation of proteins is one of the most common and complex post-translational modifications of proteins. It can endow the precursor protein with some new functions, such as its binding specificity and stability. Glycoproteins are involved in many important functions of living cells, such as mutual recognition of cells, cell differentiation, signal transduction and immune response, etc. Today, glycoproteins have been used as biomarkers for cancer diagnosis and monitoring. Therefore, deep coverage and accurate identification of glycoproteins are crucial to comprehensively study various functions of proteins. [0003] At present, the glycoproteomics strategy based on biological mass spectrometry technology has become a very com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/40
CPCG01N1/4044
Inventor 张养军钱小红夏朝双高方园焦丰龙吕雅瑶吴琼余谦
Owner ACADEMY OF MILITARY MEDICAL SCI
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