The invention relates to an extraction process for
bovine serum albumin by a graduated
heat shock method. The process comprises the following steps: 1. serum separation: removing
fibrin from healthy bovine fresh
whole blood, carrying out continuous
centrifugation to collect serum and erythrocytes for other use; 2. extraction: adding the serum and an equivalent volume of normal
saline into a reaction tank, carrying out uniform mixing at the stirring rate of 20rpm to 40rpm, adding 0.32% to 0.38% of
sodium caprylate with stirring, so as to enable the
sodium caprylate to be thoroughly bonded to
albumin; adding 0.1M dilute
hydrochloric acid, adjusting the pH to 5.8 to 6.0, heating to the temperature of 60 DEG C to 75 DEG C, carrying out heat preservation for 10 hours, carrying out filtering to remove precipitates, adjusting the pH of supernatant to 4.5 to 5.0, carrying out heat preservation, carrying out filtering to remove precipitates, and collecting
clear liquid; 3. concentrating, demineralizing and on-site sterilization
filtration: adjusting the pH of the
clear liquid to 6.8 to 7.2, carrying out cyclic sterilization, carrying out
ultrafiltration concentrating by an
ultrafiltration membrane with the molecular weight of 10,000, and carrying out
saline matter eluting, wherein the final concentration of a
protein concentrate does not exceed 15%; 4. carrying out
freeze drying to obtain crystalline
dry powder. According to the process, the used materials are simple, the cost is reduced, the process is simple and convenient, and the operation is easy.