Preparation method of human serum albumin

A technology of human serum albumin and human plasma, which is applied in the field of extracting human serum albumin, can solve problems such as waste of resources, achieve the effects of reducing demand, simplifying production technology, and improving comprehensive utilization capabilities

Active Publication Date: 2012-12-12
TONROL BIOLOGICAL PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, albumin (except genetic engineering products) used for clinical treatment is all derived from commercialized human plasma. Human plasma is used as the starting material. Due to the limitation of the production process, a large amount of human albumin remains in the tissue. In the sub-IV precipitation, it is treated as waste incineration, causing a lot of waste of resources

Method used

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  • Preparation method of human serum albumin
  • Preparation method of human serum albumin
  • Preparation method of human serum albumin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] a. Dissolution of component IV precipitation: the waste component IV precipitation separated from human plasma is used as the starting material, and the component IV precipitation is dissolved in 0.01mol / L Tris-HCl buffer solution with a pH value of 6.8 to obtain reaction solution A. Wherein: the volume of Tris-HCl buffer solution is 2 times of the precipitation volume of component IV, adjust the pH of reaction solution A to 6.8, control the temperature of reaction solution A to be -1~1°C, stir and dissolve for 5 hours, then press filter and separate to obtain Filtrate A;

[0033] b, while stirring the filtrate A, add polyethylene glycol with a molecular weight of 3000 to a concentration of 8% by mass / volume to obtain the reaction solution B, adjust the pH of the reaction solution B to 6.0, and control the temperature of the reaction solution B to be -1~ 1°C, continue to stir for 2 hours, let stand for 1 hour, press filter and separate to obtain filtrate B;

[0034] c....

Embodiment 2

[0044]a. Dissolution of component IV precipitation: the waste component IV precipitation separated from human plasma is used as the starting material, and the component IV precipitation is dissolved in 0.03mol / L Tris-HCl buffer solution with a pH value of 7.4 to obtain reaction solution A. Wherein: the volume of Tris-HCl buffer solution is 4 times of the precipitation volume of component IV, the pH of the reaction solution A is adjusted to 7.4, the temperature of the reaction solution A is controlled to be -1~1°C, the mixture is stirred and dissolved for 8 hours and separated by pressure filtration to obtain Filtrate A;

[0045] b, while stirring the filtrate A, add polyethylene glycol with a molecular weight of 4000 to a concentration of 14% by mass / volume to obtain the reaction solution B, adjust the pH of the reaction solution B to 6.5, and control the temperature of the reaction solution B to be -1~ 1°C, continue to stir for 2 hours, let stand for 1 hour, press filter and ...

Embodiment 3

[0056] a. Dissolution of component IV precipitation: using the waste component IV precipitation separated from human plasma as the starting material, the component IV precipitation was dissolved in 0.02mol / L Tris-HCl buffer solution with a pH value of 7.0 to obtain reaction solution A, Wherein: the volume of Tris-HCl buffer solution is 3 times of the precipitation volume of component IV, the pH of the reaction solution A is adjusted to 7.0, the temperature of the reaction solution A is controlled at -1~1°C, stirred and dissolved for 6 hours and separated by pressure filtration to obtain Filtrate A;

[0057] b, while stirring the filtrate A, add polyethylene glycol with a molecular weight of 3350 to a concentration of 11% by mass / volume to obtain a reaction solution B, adjust the pH of the reaction solution B to 6.2, and control the temperature of the reaction solution B to be -1~ 1°C, continue to stir for 2 hours, let stand for 1 hour, press filter and separate to obtain filtr...

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Abstract

The invention relates to a preparation method of a human serum albumin. The human serum albumin is extracted and purified from a waste component IV precipitate separated from human plasma, and thus the comprehensive utilization of plasma can be improved. The preparation method comprises the following steps of: 1, dissolving the component IV precipitate and then press-filtering and separating to obtain a filtrate A; 2, adding polyethylene glycol while stirring the filtrate A, and press-filtering and separating to obtain a filtrate B; 3, adjusting the pH of the filtrate B, controlling the temperature of the filtrate B, and press-filtering and separating to obtain a filtrate C; 4, adding the polyethylene glycol while stirring the filtrate C to obtain a reaction solution C, press-filtering and separating to obtain a precipitate; 5, dissolving the precipitate, carrying out DEAE Sepharose fast flow weak anion exchange chromatography, and ultrafiltering; and 6, adding sodium caprylate after the concentration of protein in an ultra-filtrate is diluted, adjusting the pH to 6.8 to 7.0, sterilizing and filtering, and carrying out pasteurized inactivation to obtain a human serum albumin finished product.

Description

technical field [0001] The invention relates to a method for preparing blood products, in particular to a method for extracting human serum albumin from the precipitate of waste component IV separated from human plasma. Background technique [0002] Human albumin is a single-chain protein with a simple primary structure, its molecular weight is 66250 Daltons (Da), and it consists of 585 residues. It is rich in aspartic acid and glutamic acid and lacks tryptophan. Albumin is a small molecule with a relatively large surface area. It can pass through capillary walls and intercellular space. It has strong negative charge and can reversibly combine with various molecules, so it can transmit various physiological activities between blood circulation and various body fluids. required substances. [0003] Human serum albumin is mainly used for shock caused by blood loss, trauma and burns, cerebral edema and increased brain pressure caused by brain injury, and has a good curative ef...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765C07K1/36C07K1/34C07K1/30C07K1/18
Inventor 邵恒波胡辉恒邓坤
Owner TONROL BIOLOGICAL PHARM CO LTD
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