Preparation method of human serum albumin
A technology of human serum albumin and human plasma, which is applied in the field of extracting human serum albumin, can solve problems such as waste of resources, achieve the effects of reducing demand, simplifying production technology, and improving comprehensive utilization capabilities
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Embodiment 1
[0032] a. Dissolution of component IV precipitation: the waste component IV precipitation separated from human plasma is used as the starting material, and the component IV precipitation is dissolved in 0.01mol / L Tris-HCl buffer solution with a pH value of 6.8 to obtain reaction solution A. Wherein: the volume of Tris-HCl buffer solution is 2 times of the precipitation volume of component IV, adjust the pH of reaction solution A to 6.8, control the temperature of reaction solution A to be -1~1°C, stir and dissolve for 5 hours, then press filter and separate to obtain Filtrate A;
[0033] b, while stirring the filtrate A, add polyethylene glycol with a molecular weight of 3000 to a concentration of 8% by mass / volume to obtain the reaction solution B, adjust the pH of the reaction solution B to 6.0, and control the temperature of the reaction solution B to be -1~ 1°C, continue to stir for 2 hours, let stand for 1 hour, press filter and separate to obtain filtrate B;
[0034] c....
Embodiment 2
[0044]a. Dissolution of component IV precipitation: the waste component IV precipitation separated from human plasma is used as the starting material, and the component IV precipitation is dissolved in 0.03mol / L Tris-HCl buffer solution with a pH value of 7.4 to obtain reaction solution A. Wherein: the volume of Tris-HCl buffer solution is 4 times of the precipitation volume of component IV, the pH of the reaction solution A is adjusted to 7.4, the temperature of the reaction solution A is controlled to be -1~1°C, the mixture is stirred and dissolved for 8 hours and separated by pressure filtration to obtain Filtrate A;
[0045] b, while stirring the filtrate A, add polyethylene glycol with a molecular weight of 4000 to a concentration of 14% by mass / volume to obtain the reaction solution B, adjust the pH of the reaction solution B to 6.5, and control the temperature of the reaction solution B to be -1~ 1°C, continue to stir for 2 hours, let stand for 1 hour, press filter and ...
Embodiment 3
[0056] a. Dissolution of component IV precipitation: using the waste component IV precipitation separated from human plasma as the starting material, the component IV precipitation was dissolved in 0.02mol / L Tris-HCl buffer solution with a pH value of 7.0 to obtain reaction solution A, Wherein: the volume of Tris-HCl buffer solution is 3 times of the precipitation volume of component IV, the pH of the reaction solution A is adjusted to 7.0, the temperature of the reaction solution A is controlled at -1~1°C, stirred and dissolved for 6 hours and separated by pressure filtration to obtain Filtrate A;
[0057] b, while stirring the filtrate A, add polyethylene glycol with a molecular weight of 3350 to a concentration of 11% by mass / volume to obtain a reaction solution B, adjust the pH of the reaction solution B to 6.2, and control the temperature of the reaction solution B to be -1~ 1°C, continue to stir for 2 hours, let stand for 1 hour, press filter and separate to obtain filtr...
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