The invention relates to a method for preparing a
porcine circovirus type 2
colloidal gold antibody fast test strip. In the method, a
porcine circovirus type 2 ORF2
protein is expressed by utilizing
genetic engineering, and the test strip is prepared by the principle of
enzyme-linked
immunoassay and membrane
chromatography to fast test antibodies in the blood or serum of pigs. The test strip can be widely used for clinically testing
porcine circovirus diseases, has no cross reaction with other viruses, and obtains test results 98.63 percent and 95.83 percent consistent with those obtained by the two methods of the ELISA and
neutralization test. Compared with the ELISA, a
recombinant antigen immune
colloidal gold has the remarkable advantages of
high security, no need of culturing the viruses per se, the avoidance of
virus spread caused by the operation of the viruses, large-batch preparation, simple process, low production cost, stable and homogeneous
antigen components, simple, convenient and labor-saving operation, no apparatus, high detection result specificity, high
repeatability, the short time of 15 minutes for the whole test, simple, convenient, fast and accurate operation, high sensitivity, intuition and easy result judgment.