Apparatus and method for quick detection of surface cleanness degree and microbe contamination

Abstract

The invention relates to a method and apparatus for fast testing the surface cleaning degree and microorganism pollution. The ATP sulfurylase can promote the PPi on the condition of existing adenosine-5'-phosphosulfate APS to generate ATP so as to reduction the hydrolysis pyrophosphoric acid ATP. It adds firefly luciferase stabilizer to simplify the preserving condition and adds adenosine triphosphatase restrainer to improve the test accuracy and sensibility.

Description

Technical field: [0001] The present invention relates to a reagent set and a device, in particular to a reagent set for rapid detection of surface cleanliness and microbial contamination and a device for detection using the reagent set. Meanwhile, the present invention can also be applied to the sterilized operation interface, The assembly line and liquid pollution detection of food production enterprises belong to the field of biochemistry and bioluminescence. Background technique: [0002] Food Hygiene Certification System - Hazard Critical Control Point (HACCP) is being introduced into the food industry worldwide. Many countries, such as the member states of the European Union, have listed it as a statutory measure. The aim is to include the key factors that may affect food safety into the scope of monitoring, and to use rapid methods for sanitation testing to allow time for improvement measures. The Ministry of Health of my country has also issued a guideline on "Imple...

AI Technical Summary

Problems solved by technology

[0009] However, because microorganisms are in a natural state and environment, they often do not get enough nutrients or cannot effectively use food or some nutrients on the surface under certain conditions, which will inevitably lead to bacteria consuming their own ATP to maintain Self-survival (ATP hydrolysis reaction or other enzymatic reactions will consume a lot of ATP), until the external nutrients become abundant or the external nutrients become fully utilized by bacteria or microorganisms

This means that in many cases, the fluorescence value of ATP catalyzed by firefly luciferase is very low when actually measuring the surface of microorganisms or some interfaces, but a higher pollution value is obtained by using the traditional plate counting method.

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