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Water comprehensive toxicity rapid detection method based on algae chlorophyll fluorescene

A technology of chlorophyll fluorescence and detection method, which is applied in the field of rapid detection of water comprehensive toxicity based on algae chlorophyll fluorescence, and can solve the problems of long experiment cycle, cumbersome operation, repeated experiments, etc.

Active Publication Date: 2014-04-16
ANHUI INST OF OPTICS & FINE MECHANICS - CHINESE ACAD OF SCI
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Problems solved by technology

[0004] To sum up, algae is an ideal test organism for the comprehensive toxicity of water bodies. However, most algae toxicity experiments have defects such as long test periods, repeated experiments, and cumbersome operations.

Method used

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  • Water comprehensive toxicity rapid detection method based on algae chlorophyll fluorescene
  • Water comprehensive toxicity rapid detection method based on algae chlorophyll fluorescene
  • Water comprehensive toxicity rapid detection method based on algae chlorophyll fluorescene

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Embodiment Construction

[0056] A kind of water comprehensive toxicity rapid detection method based on algae chlorophyll fluorescence, comprises the following steps:

[0057] (1) Tested algae culture

[0058] Scenedesmus obliquus was used as the test organism, and SE medium was prepared, sterilized by high temperature and high pressure, the algae were transferred to the medium, and put into the light incubator for expansion, domestication and algae transfer, so that The algae have achieved purification and synchronous growth, and the culture conditions are set as follows: light 2000-3000lux, light-dark ratio 14h:10h, pH value 8±1, temperature 25±2°C, shaking once a day, during the algae cultivation process, regularly Utilize the microscopic cell counting method to measure the biomass of algae cells, draw the algae growth curve, and finally obtain the logarithmic growth phase, and the cell concentration is at 10 4 Cell / mL level of tested algae liquid;

[0059] The experiment was carried out in a 250m...

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Abstract

The invention discloses a water comprehensive toxicity rapid detection method based on algae chlorophyll fluorescene. According to the method, toxic and sensitive unicellular scenedesmus obliquus is selected as a testing organism, an experimental group and a blank control group of a to-be-tested water sample are subjected to adaptive cultivation for 24 hours, fast-phase chlorophyll fluorescene of two groups of algae samples is measured, fluorescence parameters in the energy transfer process of main photosynthesis are analyzed and obtained, an inhibition ratio of to-be-tested water of the experimental group on the photosynthesis of algae is calculated, and the comprehensive toxicity intensity of the to-be-tested water is calculated according to the dose-effect relationship between the substance toxicity intensity and the inhibition ratio of photosynthesis. The method has the characteristics of sensitive toxicity response, simple and fast test and the like and is a new method for rapid evaluation of ecological risk and emergency monitoring of sudden pollution accidents.

Description

technical field [0001] The invention belongs to the field of environmental sciences, and in particular relates to a rapid detection method for water body comprehensive toxicity based on algae chlorophyll fluorescence. Background technique [0002] With the rapid development of my country's economy, industrial and agricultural wastewater and domestic sewage are increasing, which seriously exceed the purification ability of nature itself, and have a great impact on the aquatic ecosystem and human living environment. Due to the complexity of the chemical composition, structure and source of pollutants, as well as the antagonism, inhibition and synergy among various chemical substances, traditional physical and chemical analysis cannot directly and comprehensively reflect the comprehensive impact of various pollutants on the ecological environment . The biological toxicity detection method can synthesize the interaction of various pollutants, determine the direct relationship b...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 殷高方肖雪张玉钧赵南京段静波胡丽方丽石朝毅甘婷婷余晓娅董欣欣刘建国刘文清
Owner ANHUI INST OF OPTICS & FINE MECHANICS - CHINESE ACAD OF SCI
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