The invention provides an industrialized high-density mixed fermentation medium of bifidobacterium and lactobacillus, a fermentation culture method and a bacterial powder embedding method; the fermentation medium is: corn steep liquor dry powder 0.3-1.5%, soybean peptone 0-1.5% 1.8%, fructooligosaccharide 0‑1%, glucose 0.5‑1%, dipotassium hydrogen phosphate 0.4‑1.35%, potassium dihydrogen phosphate 0.4%, magnesium sulfate 0.015‑0.02%, L‑cysteine hydrochloride 0.05 %; or corn steep liquor dry powder 0.3‑1.5%, beef extract 0‑1%, fructooligosaccharides 0‑1%, glucose 0.5‑1%, dipotassium hydrogen phosphate 0.4‑1.35%, potassium dihydrogen phosphate 0.4%, magnesium sulfate 0.015-0.02%, L-cysteine hydrochloride 0.05%; the fermentation method includes strain activation culture, primary strain cultivation, secondary seed liquid preparation, 200L small tank cultivation in workshop and 1T large tank fermentation in workshop nourish. Compared with the traditional TPY culture medium, the culture medium formula provided by the invention has more than 10 times the number of viable bacteria and 60% lower cost, and the raw materials of the formula are easy to purchase, the ingredients are simple, and it is suitable for industrial production needs. The bacterial strain embedding technology greatly improves the ability of the bacterial strain to tolerate the human body and the external environment.