An industrialized high-density mixed fermentation culture method of bifidobacteria and lactobacillus and a bacteria powder embedding method

Abstract

The invention provides an industrialized high-density mixed fermentation medium of bifidobacterium and lactobacillus, a fermentation culture method and a bacterial powder embedding method; the fermentation medium is: corn steep liquor dry powder 0.3-1.5%, soybean peptone 0-1.5% 1.8%, fructooligosaccharide 0‑1%, glucose 0.5‑1%, dipotassium hydrogen phosphate 0.4‑1.35%, potassium dihydrogen phosphate 0.4%, magnesium sulfate 0.015‑0.02%, L‑cysteine ​​hydrochloride 0.05 %; or corn steep liquor dry powder 0.3‑1.5%, beef extract 0‑1%, fructooligosaccharides 0‑1%, glucose 0.5‑1%, dipotassium hydrogen phosphate 0.4‑1.35%, potassium dihydrogen phosphate 0.4%, magnesium sulfate 0.015-0.02%, L-cysteine ​​hydrochloride 0.05%; the fermentation method includes strain activation culture, primary strain cultivation, secondary seed liquid preparation, 200L small tank cultivation in workshop and 1T large tank fermentation in workshop nourish. Compared with the traditional TPY culture medium, the culture medium formula provided by the invention has more than 10 times the number of viable bacteria and 60% lower cost, and the raw materials of the formula are easy to purchase, the ingredients are simple, and it is suitable for industrial production needs. The bacterial strain embedding technology greatly improves the ability of the bacterial strain to tolerate the human body and the external environment.

Description

technical field [0001] The invention belongs to the technical field of probiotic product preparation, and relates to an industrialized high-density mixed fermentation medium of bifidobacteria and lactobacilli, a fermentation culture method and a bacteria powder embedding method. Background technique [0002] Probiotics are a class of active microorganisms that are beneficial to the host. They colonize the human intestinal tract and reproductive system and can produce definite health effects, thereby improving the host's micro-ecological balance and exerting beneficial effects. The general term for active microorganisms. There are a huge number of microorganisms inhabiting the human gut, and their composition and activities affect the host's immunity, mucosal development, and metabolism of nutrients and drugs. As one of the main types of probiotics, lactic acid bacteria play an important role in maintaining the balance of intestinal flora, reducing cholesterol, reducing endot...

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of high medium cost, complex industrial production method and low in vitro survival rate of bacterial powder in the current co-cultivation of probiotics, and at the same time increase the viable count of lactic acid bacteria in industrial production, the invention provides a direct-injection dual The high-density mixed fermentation medium, fermentation culture method and large-scale production fermentation method of Mycobacterium and Lactobacillus, through screening and optimizing different culture media and culture methods, and taking some comprehensive measures such as simple and practical metabolic regulation methods, make the culture medium The density of lactic acid bacteria reaches 10 10 cfu / ml

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