The invention discloses a waterproof and oxygen-insulating sealing film, a preparation method of the waterproof and oxygen-insulating sealing film, and an application of the waterproof and oxygen-insulating sealing film. The preparation method comprises the following steps: firstly, preparing graphene oxide nanoribbons (GONRs) by adopting a longitudinal multi-walled carbon nanotube oxidization cutting method, performing modification to obtain functional GONRs (K-GONRs), and then compounding the K-GONRs and an ethylene-vinyl acetate copolymer (EVA) on a film coating machine to form a film. According to a composite material thin film prepared by using the method disclosed by the invention, the K-GONRs have excellent compatibility with an EVA matrix, and meanwhile, the K-GONRs achieve good dispersion in the matrix. In addition, most of obtained laminar K-GONRs are distributed in an EVA composite thin film in parallel. By virtue of the multi-layer and parallel-distributed special structure and the close combination between a K-GONRs inserted layer and the EVA matrix, the thin film has excellent blocking performance and good acid and alkali resistance, and further improves the mechanical performance; meanwhile, the thin film is safe and environment-friendly, is particularly suitable for preparing valuable precision instrument sealing films, ice bags for packaging ice and frozen products, and food packaging films, and has wide practical application values.