The invention provides an edible fungus high dietary fiber noodle. Natural edible fungi are adopted as the raw and supplemental materials, which can provide edible fungus polysaccharide, and has the functions of enhancing immunity and strengthening constitution. Lentinus edodes and other edible fungi also contain abundant dietary fiber, thereby improving the dietary fiber content of the noodle. In addition, the invention also provides a preparation method of the noodle. Fresh lentinus edodes and other edible fungi are employed as the raw materials, by means of crushing and super-fine comminution enzymolysis, an edible fungus thick slurry can be prepared to replace water to knead dough, thus not only saving the energy consumption of drying during lentinus edodes powder preparation, but also reducing the loss of nutrition and flavor. Therefore, the technology has low energy consumption and high efficiency, and the product quality is good. Through crushing and super-fine comminution enzymolysis, the surface area of nutrient dissolution or degradation is increased, and the protein digestibility and starch digestibility are greatly enhanced. The noodle provided by the invention has hardness, elasticity and cohesiveness equivalent to those of commercially available ordinary fine dried noodles, but has dietary fiber content, protein and starch digestibility superior to ordinary fine dried noodles, and has better taste.