34results about How to "Achieve hydrolysis" patented technology

The invention provides a method for extracting hydrolyzed collagen from bovine cartilage. The method sequentially comprises the following steps of: processing fresh bovine cartilage which is obtained from a Muslim slaughter house and serves as a raw material with solution of sodium hydroxide; adding papain and alkali protease into the obtained product by stages and simultaneously adding a composite enzymolysis protecting agent into the obtained product; raising temperature for inactivating enzyme after two enzymolysis processes; decoloring by oxidation; filtering and performing ultra-filtering; collecting retained solution; and performing spray-drying on the solution to obtain the hydrolyzed collagen. In the method, the bovine cartilage of the Muslim slaughter house is used as a basic rawmaterial, and other animal derived enzyme preparations are not added during the entire process, so that the Muslim property and no pollution of the product can be ensured.

The invention relates to modified PET (Polyethylene terephthalate) polyester as well as a preparation method and application thereof, and belongs to the field of modified polyester. The modified PET polyester comprises aerogel particles and polyethylene terephthalate; the aerogel particles are dispersed between the molecular chains of the polyethylene terephthalate uniformly; the particle size of the aerogel particles is 2 to 3 microns; the content of the aerogel particles in the modified PET polyester is 1 to 10 percent by weight. Modified PET is prepared by the steps of adding the aerogel powder into glycol, putting into a grinding machine, grinding to prepare mixed slurry, mixing the mixed slurry and terephthalic acid, and performing esterification reaction and polymerization reaction. Modified PET fiber is prepared by slicing the modified PET, metering, spinning, cooling, applying oil, stretching, performing heat setting and performing winding formation. The aerogel particles are introduced before polymerization of the PET polyester, so the prepared PET has high spinnability, can produce modified PET fibers with various thickness, has wide application range and can meet the production requirement of various fabrics.

The invention relates to a method for preparing chemically crosslinked photonic crystal hydrogel with an adjustable color. The method comprises the following steps: (1) dissolving N-isopropylacrylamide, N-hydroxyethyl acrylamide, a cross-linking agent and an emulsifier into deionized water, stirring, warming and keeping warm under the condition at 20-25 DEG C in a nitrogen condition, and then adding an initiator to continue to react for 1-4 hours, and dialyzing, so as to obtain a colloidal crystal hydrogel solution; (2) concentrating the obtained colloidal crystal hydrogel solution, standing at room temperature, adjusting the pH by adopting an NaOH solution, adding a crosslinking agent, evenly mixing, centrifuging, and standing at room temperature after taking, so as to obtain the chemically crosslinked photonic crystal hydrogel. The method is simple in process and low in cost, and the prepared photonic crystal hydrogel has thermosensibility. In addition, the obtained photonic crystal hydrogel is good in stability, and free of hydrolysis, the problem that nano hydrogel is easily decomposed because of ester hydrolysis in 2-hydroxy ethyl acrylate is solved, and the defects of the photonic crystal hydrogel in application are compensated.

The invention relates to aerogel modified heat-resistant and flame-retardant low-thermal-conductivity rigid polyurethane foamed plastic and a preparation method thereof. An aerogel composite heat-resistant flame retardant is dispersed into a polyurethane body and is modified to form a polyurethane foamed material, wherein the composite heat-resistant flame retardant is formed by modifying aerogelpowder with a phosphate heat-resistant flame retardant. According to the preparation method, the composite heat-resistant flame retardant of modified aerogel is added into polyurethane foaming plastic(RPUF); the composite heat-resistant flame retardant is uniformly dispersed into an RPUF body; heating, curing and ripening are performed to obtain the heat-resistant and flame-retardant low-thermal-conductivity rigid polyurethane foamed plastic. The modified RPUF provided by the aerogel modified heat-resistant and flame-retardant low thermal conductivity rigid polyurethane foam plastic disclosedby the invention has the characteristics that the heat resistance reaches up to 153 DEG C, the thermal conductivity is lower than 0.027W / m.K, a limiting oxygen index is greater than or equal to 32 percent, and the compressive strength is 0.39 to 0.40MPa.

The invention relates to a PET (polyethylene terephthalate) polyester slice with an air purifying function as well as a preparation method and application of the PET polyester slice. The PET polyester slice is prepared from PET polyester, wherein the PET polyester is prepared from aerogel particles and polyethylene terephthalate; the aerogel particles are composite silicon aerogel particles of nanometer titanium oxide and a rare-earth solid solution. The PET polyester slice is bi-directionally stretched to prepare a PET thin film. As a nanoscale titanium dioxide photocatalyst exists in the composite silicon aerogel particles of the nanometer titanium oxide and the rare-earth solid solution in the thin film, the silicon aerogel is used for adsorbing pollutants, such as formaldehyde, and the nanometer TiO2 is used for photolyzing the pollutants, such as the formaldehyde, deposition is preformed while adsorption is performed, a long-term decomposition effect is achieved, and the polyester thin film has the performance of automatically depositing the pollutants in air. When the polyester thin film provided by the invention is paved on vehicle window glass, the air in a vehicle can be effectively purified; when the polyester thin film provided by the invention is paved on window glass or a furniture surface in a building, indoor air can be effectively purified.

The invention discloses a method for hydrolyzing vegetable fibre by solid acid, comprising the following steps: (1) adding smashed vegetable fibre and a solid-acid catalyst into water, mixing and dissolving; (2) carrying out hydro-thermal reaction at the temperature of 140-180DEG C for 6-10 hours after the smashed vegetable fibre and the solid-acid catalyst are evenly mixed and dissolved, and obtaining hydrolysate after the reaction; and (3) filtering the hydrolysate so as to separate target objects and the solid-acid catalyst, drying filter residues so as to be directly reused, and regulating the pH value of filter liquor to be within 6-8. The invention adopts solid sulfoacid with better hydrothermal stability and larger acid value to replace liquid acid, realizes the hydrolyzation of the cellulose under the moderate condition and solves the problem that the tradition catalyst is difficult to be separated from products; and after separation, the catalyst can be recycled, thereby avoiding lower economic benefit and acid contamination caused by the loss of liquid-acid catalysts.

The invention belongs to the technical field of medicine, and provides a preparation method of brain protein hydrolysate. The preparation method comprises steps of raw material treatment, papain hydrolysis, composite enzyme hydrolysis, pH adjustment, sterilization, and nano filtration. The preparation method is simple and can be easily applied to industrial production. The prepared brain protein powder has the advantages of high peptide content and high vitality.

The invention relates to application of modified PET (Polyethylene Terephthalate) polyester and belongs to the field of modified polyester. The modified PET polyester comprises aerogel particles and polyethylene terephthalate; the aerogel particles are uniformly dispersed among molecular chains of the polyethylene terephthalate; the size of the aerogel particles is 2-3 microns; and the content ofthe aerogel particles in the modified PET polyester is 1-10wt%. The modified PET polyester is prepared by the following steps of adding aerosol powder in ethylene glycol, putting a mixture in a grinder for grinding to prepare a mixed slurry, mixing the mixed slurry with terephthalic acid, and performing esterification reaction and polymerization reaction on a mixture to prepare modified PET; and performing slicing, metering, silk spurting, cooling, oil application, stretching, heat setting and winding formation to prepare modified PET fibers. By introducing the aerogel particles before polymerization of PET polyester, the prepared modified PET is good in spinnability, can be used for producing the modified PET fibers with various thicknesses so as to be wide in application range and can meet the production demands for various fabrics.

The invention relates to a method for increasing reducing sugar yield by coupling of membrane separation and lignocellulose acid hydrolysis. The method is characterized in that hydrolytic reaction and the membrane separation are coupled to realize timely separation of hydrolysate and avoid continuous degradation of the hydrolysate, so that the reducing sugar yield is increased, fermentation inhibitors are prevented from being generated effectively, and subsequent fermentation is facilitated. According to the method, ordinary filtration, micro filtration and ultrafiltration are combined for use organically, the pollution of the hydrolysate to an ultrafiltration membrane is reduced effectively; and as various filter membrane retention substances return to a reaction kettle for continuous hydrolysis, the substrate conversion efficiency and the purity of the hydrolysate are improved, and the reducing sugar yield is increased.

This invention relates to a power generating apparatus including two systems. In one of the systems, a hydrogen gas is generated by hydrolysis, and a hydrogen demanding unit generates electric power. In the other system, electric power is generated based on an oxidation-reduction chemical reaction of an electrode and ions. The power generating apparatus includes a hydrogen release unit with a hydrogen generating container within which a functional material has been mounted. Alternatively, a construction may be adopted in which the functional material is mounted on negative and positive electrodes and a battery element including the negative and positive electrodes and an electrolyte is provided within the container. The power generating apparatus further includes a hydrogen generating unit including a liquid container which generates a hydrogen gas both by hydrolysis, in which the functional material is reacted with water or an aqueous solution (an electrolysis solution), and by binding of hydrogen atoms which settle among crystals due to a quality change of the functional material by the hydrolysis, a first electrogenic unit in which the battery element in the hydrogen generating container generates electricity, a second electrogenic unit in which the hydrogen demanding unit generates electricity by a hydrogen gas produced in the hydrogen generating container, a control unit which applies electric power generated by the first electrogenic unit to a load device and, based on the load current amount, controls the amount of the hydrogen gas generated by the hydrogen generating unit.

The invention provides a fixed bed pipe type reaction device which can realize the catalytic hydrolysis of liquid glycan and the concentration of a sugar liquid at lower reaction temperature. The fixed bed pipe type reaction device comprises a raw material storage tank, a fixed bed pipe type reactor and a sugar liquid storage tank, wherein the raw material storage tank is provided with an agitating device and a device for mechanically sealing the agitating device and is also provided with an air outlet and a liquid outlet; a raw material input pipe is communicated with the raw material storage tank, and a raw material output pipe is communicated with the fixed bed pipe type reactor; an output end of the fixed bed pipe type reactor is communicated with a liquid discharge pipe, a return pipe and a sugar liquid delivery pipe respectively through valves, and the return pipe and the sugar liquid delivery pipe are respectively communicated with the raw material storage tank and the sugar liquid storage tank; and the sugar liquid storage tank is provided with a return concentrating pipe to be communicated with the raw material storage tank. By using the device provided by the invention, the biomass pretreatment step can stay to the extent of generating glycan without further reaction to generate monosaccharide, the energy consumption of the biomass hydrolysis step is greatly reduced,and the economical efficiency of cellulosic ethanol fuel is improved.

The invention belongs to the technical field of medicinal oil refining, and discloses a method for accurately controlling the content of stearic acid in soybean oil by using a mixed filler. The methodcomprises the following steps: A. physically and uniformly mixing lipase, inorganic base, a filter aid and an adsorbent to form a mixed filler, the lipase being Sn-1, 3-site specific lipase; B, enabling soybean oil to flow through the mixed filler to obtain refined soybean oil. The method solves the problems that the stearic acid content cannot be accurately controlled and the requirement for preparing a high-quality fat emulsion preparation cannot be met in the traditional soybean oil production process.

The invention discloses a method for synthesizing achiral glycidol, aims to provide a new method suitable for industrialized production, being environment-friendly and being higher in yield, and belongs to the field of chemical synthesis. The method comprises the following steps of: taking achiral vicinal diamine and ortho-hydroxybenzaldehyde as raw materials, and continuously performing multistep reaction of condensation, transition metal coordination, oxidization and the like, according to the one-pot method, thereby efficiently synthesizing a plurality of achiral Salen catalysts. Under the action of the achiral Salen catalysts, the hydrolysis of racemized epoxy chloropropane is realized; the obtained glycerin chlorohydrin is subjected to alkaline cyclizing reaction, thereby synthesizing the achiral glycidol with high yield; and in the whole process flow, the operation is convenient, the products are completely racemized and no optical activity exists.

The invention is applicable to the technical field of lignocellulose resource conversion and utilization and biorefinery, and provides a method for preparing microbial oil by utilizing straw resources, which comprises the following steps: continuously feed-batch supplementing materials to carry out high-solid-phase enzyme hydrolysis on pretreated straws, so that the sugar concentration of the final hydrolysate reaches more than 300g / L; through two times of quantitative water washing, directional preparation of medium-concentration and low-concentration sugar solutions is achieved, the requirement for the performance of filtering or centrifuging equipment in the technological process is lowered, the high-concentration sugar solution, the medium-concentration sugar solution and the low-concentration sugar solution are matched with fed-batch fermentation, and efficient combined utilization is achieved in fed-batch grease fermentation. Besides, soluble oligosaccharides accounting for about 10-20% of the total sugar mass can be generated through high solid phase enzyme hydrolysis, and the oligosaccharides can serve as a strong inducer to induce enzyme-producing fungi to produce a large amount of cellulase for high solid-to-liquid ratio enzyme hydrolysis, so that self-production and self-use of the cellulase are realized, and the enzyme cost is remarkably reduced.

The invention belongs to a plugging agent for temporary plugging in oil and gas well repairing. The plugging agent comprising the following raw materials in parts by mass: 20 to 40 parts of a polymeric monomer, 5 to 10 parts of a biopolymer, 0.1 to 1.0 part of a cross-linking agent, 50 to 70 parts of a reinforcing agent, 0.01 to 0.05 part of a gel breaker and 0.001 to 0.003 part of an initiator, wherein the polymeric monomer is selected from a mixture of acrylamide and methacrylamide; the biopolymer is one selected from xanthan gum and guar gum; the cross-linking agent is selected from ethylene glycol dimethacrylate; the reinforcing agent is one or a mixture of more than one selected from potassium chloride, sodium chloride, sodium nitrate and potassium nitrate; the gel breaker is a mixture of benzoyl peroxide and ferrous sulfate, and a molar ratio of benzoyl peroxide to ferrous sulfate is (1-2): 1; and the initiator is 1-((cyano-1-methylethyl)azo)formamide. The plugging agent has the advantages that pressure-bearing strength is good, self-degradation release can be realized, and damage to a stratum is small.

The invention belongs to the technical field of medicine and provides a preparation method of brain protein hydrolyzate, which comprises the steps of raw material treatment, papain hydrolysis, compound enzyme hydrolysis, pH adjustment, sterilization and nanofiltration. The preparation method of the invention is simple and easy for industrialized production; the cerebroprotein powder prepared by the method has high peptide content and high activity.

The invention discloses a method for purifying and directionally immobilizing histidine tag protein and application. According to the method disclosed by the invention, tannic acid, metal ions and a hydrophilic anti-protein adhesion polymer are self-assembled and modified on the surface of a carrier to obtain a material surface which is efficiently chelated with cobalt ions, so that one-step purification and immobilization of the histidine tag protein is realized; a ligand of trivalent cobalt has inertia and divalent cobalt ions are oxidized into trivalent cobalt ions so that the stability of the immobilized protein can be improved. The method disclosed by the invention has the advantages of simple process and low price; a separation and purification or immobilization operation process for the histidine tag protein of the method is simple and effective, so that the technology has a wide application prospect.

The present invention discloses a preparation method of an efficient photocatalyst. The method comprises the steps of: the step 1, putting an aluminum oxide base into distilled water, carrying out ultrasonic dispersion for 1-2 hours, performing airing, uniformly spraying diluted hydrochloric acid on the surface, and carrying out secondary drying, so as to obtain an aluminum oxide base material with a roughened surface; the step 2, adding titanium tetrabutoxide into absolute ethyl alcohol and performing uniform stirring, so as to obtain a titanium alcohol solution; the step 3, soaking the aluminum oxide base material into the titanium alcohol solution, performing uniform lifting and drying, and performing repeating for 5-10 times to obtain coated aluminum oxide; the step 4, putting the coated aluminum oxide into a reaction kettle for sintering for 2-4 hours to obtain a titanium dioxide film, then adding the titanium dioxide film into a hydrogen reaction kettle, and carrying out sealed reduction reaction for 20-40 minutes to obtain a titanium monoxide-titanium dioxide composite film; and the step 5, spraying the titanium tetrabutoxide on the surface of the film, and then performing high-temperature sintering in a nitrogen environment for 2-5h to obtain the photocatalytic film. The oxygen deficiency of titanium monoxide is utilized, and titanium dioxide is used as a coating layer,so that the anoxic activity of titanium dioxide is greatly improved, and the overall photocatalytic performance is effectively improved.

The invention relates to a PET (polyethylene terephthalate) polyester slice with an air purifying function as well as a preparation method and application of the PET polyester slice. The PET polyester slice is prepared from PET polyester, wherein the PET polyester is prepared from aerogel particles and polyethylene terephthalate; the aerogel particles are composite silicon aerogel particles of nanometer titanium oxide and a rare-earth solid solution. The PET polyester slice is bi-directionally stretched to prepare a PET thin film. As a nanoscale titanium dioxide photocatalyst exists in the composite silicon aerogel particles of the nanometer titanium oxide and the rare-earth solid solution in the thin film, the silicon aerogel is used for adsorbing pollutants, such as formaldehyde, and the nanometer TiO2 is used for photolyzing the pollutants, such as the formaldehyde, deposition is preformed while adsorption is performed, a long-term decomposition effect is achieved, and the polyester thin film has the performance of automatically depositing the pollutants in air. When the polyester thin film provided by the invention is paved on vehicle window glass, the air in a vehicle can be effectively purified; when the polyester thin film provided by the invention is paved on window glass or a furniture surface in a building, indoor air can be effectively purified.

The invention provides a fixed bed pipe type reaction device which can realize the catalytic hydrolysis of liquid glycan and the concentration of a sugar liquid at lower reaction temperature. The fixed bed pipe type reaction device comprises a raw material storage tank, a fixed bed pipe type reactor and a sugar liquid storage tank, wherein the raw material storage tank is provided with an agitating device and a device for mechanically sealing the agitating device and is also provided with an air outlet and a liquid outlet; a raw material input pipe is communicated with the raw material storage tank, and a raw material output pipe is communicated with the fixed bed pipe type reactor; an output end of the fixed bed pipe type reactor is communicated with a liquid discharge pipe, a return pipe and a sugar liquid delivery pipe respectively through valves, and the return pipe and the sugar liquid delivery pipe are respectively communicated with the raw material storage tank and the sugar liquid storage tank; and the sugar liquid storage tank is provided with a return concentrating pipe to be communicated with the raw material storage tank. By using the device provided by the invention, the biomass pretreatment step can stay to the extent of generating glycan without further reaction to generate monosaccharide, the energy consumption of the biomass hydrolysis step is greatly reduced,and the economical efficiency of cellulosic ethanol fuel is improved.

The invention discloses a deodorization and sterilization device for dairy products. The deodorization and sterilization device comprises an electrolytic tank, a sterilization tank and a communicating pipe. The sterilization tank comprises a tank body and a sterilization device, the tank body is of a cylinder structure with two sealed ends, and a sealing structure on the top of the tank body is detachable; the sterilization device comprises an ultraviolet lamp, a light transmittance waterproof cover and a control device, and the ultraviolet lamp is arranged in the tank body and is fixed to the bottom of the tank body; the light transmittance waterproof cover is formed on the surface of the ultraviolet lamp; the control device is electrically connected with the ultraviolet lamp, so that the ultraviolet lamp can be switched on and off under the control of the control device; electrodes in the electrolytic tank are flexible electrodes, base plates of the flexible electrodes are made of flexible polyethylene terephthalate glycol, and zeolite particle layers with diaphorase and metal oxide layers are sequentially coated on the base plates from the inside to the outside. The deodorization and sterilization device has the advantages that the ultraviolet lamp with the wavelength of 320nm is arranged in the sterilization tank, accordingly, large quantities of nonpathogenic microorganisms in the dairy products can be killed, the shelf lives of the dairy products can be prolonged, and large quantities of contaminating microorganisms can be prevented from being ingested into human bodies.

The invention discloses an ionic liquid-complex microbial inoculum system which comprises ionic liquid and a complex microbial inoculum. The ionic liquid and the complex microbial inoculum form a coupling system for enzymolysis of lignocellulose; the ionic liquid contains 1-ethyl-3-methylimidazole ferric chloride salt; and the complex microbial inoculum comprises fermentation liquor of wet cellulomonas, streptomyces albus, candida tropicalis and white rot fungi. In an enzymolysis test, the highest cellulase activity of enzymatic hydrolysate is 185.1 U.mL<1>, the degradation weight loss rate ofgarden biomass reaches 52.6% and is respectively increased by 59.0% and 66.5% with respect to that of a only complex microbial inoculum, and lignin content of enzymolysis residues is reduced by 35.2%. In the enzymolysis application, the enzymolysis period of the garden biomass is 18 to 26 days, the high-temperature period lasts for 12 to 21 days, the highest temperature reaches 62 to 72 DEG C, the final degradation weight loss rate is 47.2 to 54.2%, and the lignin content is reduced to 8.5 to 10.6%. The ionic liquid-complex microbial inoculum system is simple and convenient in preparation process and outstanding in enzymolysis efficiency, and can meet the social requirements of intensive and resource treatment and utilization of agriculture and forestry biomass and the development conceptof green circulation.

The invention belongs to the technical field of feed additives, and particularly relates to an alpha-galactosidase soft capsule feed additive and a preparing method thereof. The method includes the following steps that alpha-Gal and beta-CD are added into a phosphate buffered solution, and the mixed solution is homogenized and stands for a reaction to a reaction endpoint to obtain alpha-Gal-beta-CD additive solution; 2, B-type gelatin is added into water, and glycerinum, preservative and titanium dioxide are added after the B-type gelatin is swelled fully and are mixed uniformly to conduct colloidizing to obtain glue stock; 3, filling is conducted. The inhibition action of cyclodextrin inhibitor to alpha-Gal is used, so that alpha-Gal enzyme activity in animal feed is reduced and then improved, and alpha-Gal in the feed is more suitable for having the catalysis effect in the digestion period.

The invention relates to a method for increasing reducing sugar yield by coupling of membrane separation and lignocellulose acid hydrolysis. The method is characterized in that hydrolytic reaction and the membrane separation are coupled to realize timely separation of hydrolysate and avoid continuous degradation of the hydrolysate, so that the reducing sugar yield is increased, fermentation inhibitors are prevented from being generated effectively, and subsequent fermentation is facilitated. According to the method, ordinary filtration, micro filtration and ultrafiltration are combined for use organically, the pollution of the hydrolysate to an ultrafiltration membrane is reduced effectively; and as various filter membrane retention substances return to a reaction kettle for continuous hydrolysis, the substrate conversion efficiency and the purity of the hydrolysate are improved, and the reducing sugar yield is increased.

The invention relates to a method for preparing chemically crosslinked photonic crystal hydrogel with an adjustable color. The method comprises the following steps: (1) dissolving N-isopropylacrylamide, N-hydroxyethyl acrylamide, a cross-linking agent and an emulsifier into deionized water, stirring, warming and keeping warm under the condition at 20-25 DEG C in a nitrogen condition, and then adding an initiator to continue to react for 1-4 hours, and dialyzing, so as to obtain a colloidal crystal hydrogel solution; (2) concentrating the obtained colloidal crystal hydrogel solution, standing at room temperature, adjusting the pH by adopting an NaOH solution, adding a crosslinking agent, evenly mixing, centrifuging, and standing at room temperature after taking, so as to obtain the chemically crosslinked photonic crystal hydrogel. The method is simple in process and low in cost, and the prepared photonic crystal hydrogel has thermosensibility. In addition, the obtained photonic crystal hydrogel is good in stability, and free of hydrolysis, the problem that nano hydrogel is easily decomposed because of ester hydrolysis in 2-hydroxy ethyl acrylate is solved, and the defects of the photonic crystal hydrogel in application are compensated.

The invention discloses a preparation method of a silica aerogel precursor and solid silica aerogel. The preparation method is implemented at normal temperature and normal pressure, and is short in process time, suitable for industrialization and capable of improving the toughness and heat resistance temperature of the silica aerogel. The preparation method of the silica aerogel precursor includes the first step of preparation of a silicon source and solvent mixed solution, the second step of solation, the third step of gelation, the fourth step of ageing, the fifth step of solvent replacement and the sixth step of surface modification. The preparation method of the rare-earth-toughened solid silica aerogel includes the steps that the prepared silica aerogel precursor is put into a drying kettle, nitrogen is introduced into the drying kettle to expel oxygen till the oxygen content in the drying kettle is smaller than 3%, and then materials in the drying kettle are subjected to microwave vacuum drying; after drying, the solid powdery silica aerogel is obtained.

The invention belongs to the related technical field of crack repair, and discloses a high-tolerance environment-friendly microbial modifier and a preparation method and application thereof, and the method comprises the following steps: carrying out centrifugal treatment on a bacterial suspension obtained after enrichment culture of bacillus pasteurii or a last-generation bacterial liquid to obtain a bacterial precipitate and a bacterial supernatant; sequentially carrying out cell disruption treatment and centrifugal treatment on part of the bacterial precipitate to obtain cell disruption liquid; mixing the bacterial supernate, the other part of bacterial precipitate and the cell breaking liquid to obtain a bacterial enzyme mixture; inoculating the bacterial enzyme mixture into the liquid culture medium, and culturing to obtain bacterial liquid; mixing the bacterial liquid with first-concentration urea to obtain a reagent A liquid; and mixing the calcium source solution with the second-concentration urea to obtain a reagent B solution. The method can be applied to restoration of engineering bodies with severe microbial growth environments such as saline-alkali soil, the crack restoration effect is remarkably improved, and the method has a good application prospect.