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A kind of preparation method of cerebroprotein hydrolyzate

A technology of brain protein hydrolyzate and bacterial protease, which is applied in the field of medicine, can solve problems such as protein denaturation and complicated procedures, and achieve the effects of high activity, increased product content, and high peptide content

Active Publication Date: 2018-03-06
西藏易明西雅医药科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] CN200410022091.5 discloses a preparation method of cerebroprotein hydrolyzate. The invention prepares cerebroprotein hydrolyzate by successively using pepsin and trypsin for enzymolysis. problems with protein denaturation

Method used

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  • A kind of preparation method of cerebroprotein hydrolyzate
  • A kind of preparation method of cerebroprotein hydrolyzate
  • A kind of preparation method of cerebroprotein hydrolyzate

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0028] Experimental Example 1 Animal Pharmacodynamics Test

[0029] Test drug: commercially available compound cerebroprotein hydrolyzate sheet (purchased from Dalian Beier Pharmaceutical Co., Ltd.), the sample that the embodiment of the present invention 5 makes, is made into the solution of required concentration with distilled water, for oral administration

[0030] use.

[0031] 1) Effects on the hypobaric hypoxic state of mice

[0032] Take 150 mice of 18-21g, half male and half male, and randomly divide them into 3 groups, and administer them by intragastric administration according to the drug dosage shown in Table 1. After 30 minutes, put the three groups into the vacuum chamber at the same time, airtight, and decompress Vacuum was applied, and when more than half of the mice in the control group died, observation was continued for 30 minutes after ventilation, and the number of dead animals in each group was recorded.

[0033] The results are shown in Table 1.

[0...

experiment example 2

[0043] Experimental Example 2 Determination of Peptide Content in Cerebroprotein Hydrolyzate

[0044] 1. Experimental drug

[0045] Take the samples prepared according to the methods of Example 3, Example 5, Example 8 and CN200410022091.5.

[0046] 2. The method for measuring the cerebroprotein hydrolyzate content (in amino nitrogen) in the cerebroprotein hydrolyzate

[0047] Take an appropriate amount of sample (approximately equivalent to 50 mg of peptide), weigh it accurately, put it in a digestive tube, add an appropriate amount of hydrochloric acid (so that the test sample is completely submerged and does not exceed 2 / 3 of the volume of the container), fill the seal with nitrogen, and hydrolyze it at 110°C for 20 hours, let cool, unsealed, transfer the whole amount of the hydrolyzate to an evaporating dish, evaporate to dryness in a water bath, add water to dissolve the residue and dilute to an appropriate concentration, as the test solution for the determination of tota...

Embodiment 1

[0068] a) Raw material processing: Remove the capsule and blood vessels on the surface of the freshly preserved pig brain, cut it into small pieces, soak it in 0.5% sodium hydroxide solution for 48 hours, take it out and wash it, drain the water, and set aside;

[0069] b) Enzyme hydrolysis: add water to the reaction kettle containing the pig brain, add papain extracted from plants at a ratio of 10000U / g substrate, stir, and enzymolyze for 4 hours;

[0070] c) Compound enzyme hydrolysis: add compound enzyme to the product obtained in step b), stir, and enzymatically hydrolyze for 5 hours;

[0071] d) Sterilization: adjust the pH of the product obtained in step c) to 6, raise the temperature to 85° C., inactivate the enzyme for 8 minutes, add activated carbon after cooling to room temperature, stir for 4.8 hours, and then filter to obtain the filtrate;

[0072] e) concentration: the filtrate obtained in step d) is ultrafiltered with a 600Da nanofiltration membrane, and the rete...

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Abstract

The invention belongs to the technical field of medicine and provides a preparation method of brain protein hydrolyzate, which comprises the steps of raw material treatment, papain hydrolysis, compound enzyme hydrolysis, pH adjustment, sterilization and nanofiltration. The preparation method of the invention is simple and easy for industrialized production; the cerebroprotein powder prepared by the method has high peptide content and high activity.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a preparation method of cerebroprotein hydrolyzate. Background technique [0002] Cerebroprotein hydrolyzate drugs are one of the most widely used biochemical drugs in recent years. It occupies an important position in related research fields and markets at home and abroad. Cerebroprotein hydrolyzate, as an active brain polypeptide substance extracted from healthy and fresh animal brain tissue by multi-enzyme hydrolysis, contains a variety of essential amino acids for human brain and important elements such as cephalin, lecithin, and peptide nerve growth factor. It acts on the central nervous system in various ways, regulates and improves the metabolism of neurons, promotes the formation of synapses, induces the differentiation of neurons, and further protects nerve cells from various ischemia and neurotoxin damage. It can pass through the blood-brain barrier, prom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/01A61K35/30A61P3/02A61P25/00A61P39/00
Inventor 曹捷高帆周战尚磊
Owner 西藏易明西雅医药科技股份有限公司
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