The invention relates to the technical field of sterilization and disinfection equipment, in particular to an ultraviolet pulse sterilization, disinfection and purification all-in-one machine, a sterilization and purification method and a computer readable storage medium. The ultraviolet pulse sterilization, disinfection and purification all-in-one machine comprises a machine body and a disinfection bin arranged in the machine body; a filtering and purifying chamber is arranged in the machine body, a disinfection suction inlet and a disinfection outlet which communicate with the filtering andpurifying chamber are formed in the disinfection bin, and an air inlet and an air outlet which communicate with the filtering and purifying chamber are formed in the machine body; a xenon ultravioletlamp and a circulating air mechanism are arranged in the disinfection bin, and a dust filtering device is arranged in the filtering and purifying chamber; and the ultraviolet pulse sterilization, disinfection and purification all-in-one machine further comprises a boosting module and a trigger which are electrically connected with the xenon ultraviolet lamp separately. Through the ultraviolet pulse sterilization, disinfection and purification all-in-one machine, sterilization and disinfection can be performed on air, the air is purified, and man-machine coexistence can be achieved.