The invention relates to a lipopeptide-containing composite oil displacement agent and a use method thereof. The use method comprises the steps that S1, a weak base solution is injected into a stirring chamber through a first injection port to be stirred, a first composition formed by petroleum sulfonate and lipopeptide is injected into the stirring chamber through a second injection port to be stirred with the weak base solution to form a second composition, a polymer is injected into the stirring chamber through a third injection port, and the polymer is mixed and stirred with the second composition to form a lipopeptide-containing composite oil displacement agent; S2, a liquid conveying pump is started, the lipopeptide-containing composite oil displacement agent is conveyed to a liquid injection pipe through a detection pipe, a regulating valve is opened by a central control unit, and the lipopeptide-containing composite oil displacement agent is injected into the natural rock core; and S3, the central control unit controls an oil extraction device to extract oil. According to the use method, the central control unit is arranged, the input amount of different materials is controlled by arranging different injection ports according to the real-time viscosity of the formed lipopeptide-containing composite oil displacement agent, and the oil recovery rate is increased by adjusting the conveying pressure of the input pump and the opening area of the liquid injection pipeline adjusting valve.