36 results about "NS - Normal saline" patented technology

The invention relates to a method for preparing a trivalent inactivated vaccine for preventing mink hemorrhagic pneumonia. The method is characterized by comprising the following concrete steps: separating serum G pseudomonas aeruginosa SD302, serum C pseudomonas aeruginosa SD312 and serum I pseudomonas aeruginosa SD403 from an ill mink body; storing bacteria by adopting a freeze-drying mode; respectively putting the three serum pseudomonas aeruginosa into PYG culture medium containing 2% fetal calf serum to cultivate at 37 DEG C; then carrying out enlarge cultivation, and carrying out inactivated treatment on a bacterium solution; diluting the inactivated bacterium solution until the concentration is 8.0*10<9> CFU / mL by using normal saline according to the bacteria content; mixing the three diluted inactivated bacterium solutions with 40% aluminum glue solution according to the volume ratio of 1:1:1:1; adding thiomersalate of which the final concentration is 0.004%, and mixing to obtain the trivalent inactivated vaccine. The method is safe, multi-titer, good in immunogenicity, good in specificity and the like, and is moderate in price, and applicable to substratum or clinical prevention of the mink hemorrhagic pneumonia.

The invention provides a detection system for intestinal feces. The detection method of the system includes: step 1, adding physiological saline and feces into the first centrifugal chamber; step 2, the stirring device of the first centrifugal chamber enters the stirring mode; step 3. The second liquid level sensor group starts to detect the liquid level height in the second centrifugal chamber; Step 4, the filtrate enters the second centrifugal chamber for centrifugation, and the primary centrifugal sediment after removing the supernatant enters the transfer chamber; Step 5, the second centrifugal chamber enters the emptying operation; Step 6, the secondary centrifugal sediment in the transfer chamber is returned to the first centrifugal chamber for centrifugation; Step 7, the transfer chamber enters the emptying operation; Step 8, the first control valve is opened again , the three centrifugal sediments enter the second centrifugal chamber; the physiological saline in the output storage bin flows into the second centrifugal chamber, and the second centrifugal chamber performs centrifugal work; Step 9, the fecal bacteria suspension that meets the requirements in the second centrifugal chamber enters the second centrifugal chamber Transit chamber storage.

The invention relates to a biological immune preparation for strengthening a goatpox vaccine antibody and a manufacturing method of the biological immune preparation. The steps adopted in the method include that the fresh spleen of a healthy yak is selected, after fat, the envelope and the internal connective tissue are removed, the spleen is subjected to freezing preservation, the yak spleen obtained after freezing preservation is taken out and melted and is minced through a tissue stamp mill, after sterile tri-distilled water with the same weight is added, the high-speed tissue stamp mill is used for conducting homogenizing at a high speed for two times, and homogenizing is conducted 10 min each time; and prepared homogenate is frozen at the temperature of minus 20 DEG C, freezing and thawing are conducted repeatedly for five times, centrifuging is conducted after the last time of thawing, supernatant is taken and put into a dialysis bag, the supernatant is dialyzed overnight under the environment of 0 DEG C to 4 DEG C through pyrogen-free cold normal saline, dialysate outside the bag is collected and is subjected to filtration sterilization through a filtering film, and the dialysate is subjected to sterile subpackaging and is then subjected to freezing preservation. After the biological immune preparation enters an organism, the effect of the biological immune preparation can be rapidly developed and can last for several months or even longer, the immune antibody level of the vaccine is effectively improved, the biological immune preparation can be rapidly and completely absorbed by the organism without any residue, weight increasing is obvious after the biological immune preparation is injected into the organism, and a goat can be lively in spirit.

The invention discloses a method for constructing an azoospermia mouse model, which comprises the following steps: (1) providing a sexually mature male mouse with a body weight of 25-35 g; (2) dissolving busulfan in DMSO and diluting it with normal saline (3) Alcohol disinfection of the lower abdomen of the mouse, after drying, draw the diluted solution obtained in step (2) and insert the needle, and after withdrawing to confirm that it is correct, complete the first intraperitoneal injection; after that, inject 3 times at intervals within the same day, After 35-37 days, the mouse model of azoospermia was obtained. The method of the present invention has high molding rate and good stability; according to the present invention, 84 C57BL / 6 mice have been accumulatively completed modeling of azoospermia, and the total number of moldings identified by testis HE staining is 84 / 84, and the molding rate is 84 / 84. 100%; adopt the method of the present invention to build the model, the mortality rate of the mice is 0%; according to the method of the present invention, the total number of deaths of 84 C57BL / 6 mice that have been injected is 0, and the mortality rate is 0%.

The invention relates to a method for preparing a human anatomical visceral specimen. The method comprises the following steps: step one, pouring normal saline into a viscus through an artery for washing and cleaning; step two, pouring visceral and immersing the visceral artery into pretreatment liquid for 12 to 24 hours, and then carrying out vacuum treatment; step three, carrying out pre coolingon the viscus for 3 to 5h at a temperature of 2 DEG C, and immersing the cooled viscus into a dehydration solution for 16 to 24h; step four, immersing the viscus into a sealed container with preservation solution for 36 to 72h and carrying out gradient vacuum treatment; step five, removing excess material of the visceral outer layer to obtain a softened specimen; and step six, placing the softened specimen into a sealed box filled with a curing agent for 72 to 96 hours to obtain a cured specimen. The method is simple and easy. Since the pretreatment liquid and the preservation liquid are notirritating, the operator is protected from being injured; the softened visceral specimen is not easy to damage and can be used for long time; and the visceral specimen has the natural color and soft texture and has no mildew phenomenon.