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Stem cell preparation and application of stem cell preparation in preparing drug for treating chronic nephritis

A stem cell preparation, a technology for chronic nephritis, applied in the field of medicine, can solve the problems of obvious side effects, cure the symptoms but not the root cause, and the effect is unsatisfactory, and achieve the effect of reducing the content of urine protein

Inactive Publication Date: 2017-01-04
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the treatment of chronic nephritis, although many treatment modalities have been elucidated, the development of the disease cannot be prevented
The treatment of chronic nephritis is currently mostly using hormone therapy in Western medicine, but hormones have a strong ability to cover up the disease, and the side effects are obvious.

Method used

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  • Stem cell preparation and application of stem cell preparation in preparing drug for treating chronic nephritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Preparation of umbilical cord mesenchymal stem cells

[0037] After rinsing the umbilical cord with PBS solution containing 200U / m double antibody for 3 times, cut it into 2cm long pieces, peel off the arterial and venous tubes and adventitia, and cut them into pieces of 1-2mm 2 Small pieces, evenly attached to a 10cm culture dish, placed upside down in a 5% CO2 incubator at 37°C for 1-2 hours; the medium is X-VIVO15 serum-free medium.

[0038] Add X-VIVO15 serum-free medium containing 10 ng / mL EGF, culture at 37°C with 5% CO2 for 2 to 3 days, then change the medium in half, continue to culture for 6 to 8 days, and discard the tissue after the cells crawl out of the tissue continue to train;

[0039] After growing to 80% confluence, discard the culture medium, wash once with PBS, add 0.25% trypsin to digest for 1-2 minutes, add FBS to stop digestion, and collect umbilical cord mesenchymal cells by centrifugation at 400g for 5 minutes.

[0040] Umbilical cord...

Embodiment 2

[0044] Preparation of embodiment 2 stem cell preparation

[0045] Collect the P2 and P3 generation stem cells screened in Example 1, and resuspend them with physiological saline to a cell density of 2×10 6 cell / mL.

Embodiment 3

[0046] Preparation of embodiment 3 stem cell preparation

[0047] Collect the P2 and P3 generation stem cells screened in Example 1, resuspend with physiological saline to a cell density of 1×10 6 cell / mL.

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Abstract

The invention relates to the technical field of medicines, in particular to a stem cell preparation and an application of the stem cell preparation in preparing a drug for treating chronic nephritis. The provided stem cell preparation is prepared through resuspension of umbilical cord mesenchymal stem cells by the aid of normal saline, the content of urine protein of a patient and the number of urine erythrocyte are reduced by inhibiting expression of T cell surface antigen CD8 and secretion of INF-gamma and up-regulating secretion of IL-4 of T lymphocyte of peripheral blood, so that the effect for treating chronic nephritis is realized, the effective rate of treatment can reach 88%, and the remission rate can reach 54%.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a stem cell preparation and its application in preparing medicine for treating chronic nephritis. Background technique [0002] Chronic nephritis: chronic glomerulonephritis, which refers to proteinuria, hematuria, hypertension, and edema as the basic clinical manifestations. A group of glomerulopathies that tend to deteriorate renal function and eventually develop into chronic renal failure. [0003] The pathogenesis of most chronic nephritis is immune-mediated inflammation. In addition, non-immune and non-inflammatory mechanisms play an important role in the development of the disease. For example, long-term compensatory nephrons are in the "three high" states of high blood perfusion, high filtration and high transmembrane pressure, which leads to healthy kidneys over time. The pellet hardens. Chronic nephritis can occur at any age, but it is mainly young and middle-aged, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/51A61K9/10A61P13/12
CPCA61K9/0019A61K9/10A61K35/51
Inventor 葛啸虎陈海佳王一飞应杰张维敏
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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