Composition for protecting adipose tissue and preparation method and application of composition

A technology for adipose tissue and use, applied in the field of compositions for protecting adipose tissue, can solve the problems of loss of experimental reagent consumables, failure of ADSC culture, high time and economic cost, etc., and achieves the maintenance of traits and differentiation potential, good market application value, and shortened The effect of incubation time

Active Publication Date: 2017-05-31
中科领康(广州)医疗有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a certain risk of bacterial contamination in the collection of adipose tissue by liposuction. Bacterial contamination will lead to the failure of ADSC culture, resulting in the waste of adipose tissue
The failure of the experiment also caused the loss of experimental reagent consumables
And even if the fat can be re-collected by liposuction, it will increase the patient's pain and physical damage, resulting in time and economic losses.
In addition, ADSCs are also cultured by the natural adherent culture method of adipose tissue. The cells cultured in adipose tissue without protective agent have good morphology, strong activity, and strong proliferation ability, but it takes a long time, consumes a lot of medium, and the time and economic costs higher

Method used

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  • Composition for protecting adipose tissue and preparation method and application of composition
  • Composition for protecting adipose tissue and preparation method and application of composition
  • Composition for protecting adipose tissue and preparation method and application of composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1 Preparation of protective agent of the present invention and its protective effect for isolated fat

[0060] One, prepare protective agent of the present invention to preserve adipose tissue

[0061] For the formula shown in the table below, dissolve penicillin and streptomycin in sodium chloride injection under sterile conditions, then add L-glutamine to dissolve it, then add DMEM solution, mix the mixture evenly, and then Aseptically dispense into sterilized collection bottles, 30ml per bottle, and add 30ml of collected adipose tissue.

[0062] The formula of protecting agent of the present invention

[0063]

[0064] In the control group, 30ml of adipose tissue was preserved without protective agent.

[0065] 2. Antibacterial experiment of protective agent

[0066] The micro-broth method was used for the determination of the antibacterial effect, and the maximum concentration of the test drug was 50 μg (U) / ml, which was half of the actual drug conc...

Embodiment 2

[0127] Example 2 Various performance tests after the adipose tissue protected by the protective agent of the present invention is used for culturing cells

[0128] One, prepare protective agent of the present invention

[0129] According to the preparation method of Example 1, Group 2, penicillin and streptomycin were dissolved in sodium chloride injection under aseptic conditions, then L-glutamine was added to dissolve them, and then DMEM solution was added. Mix the mixture evenly and make it into a mixed solution containing 125000U of penicillin, 125000U of streptomycin, 365.3mg of L-glutamine, 500ml of DMEM solution and 500ml of sodium chloride injection per liter, and then aseptically dispense it until it is sterilized. Bacteria collection bottle, 30ml per bottle.

[0130] 2. Adipose stem cell growth experiment

[0131] ⑴Experimental method:

[0132] Adipose tissue preserved in sterile collection bottles with protective agent was used as group A, and adipose tissue pres...

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PUM

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Abstract

The invention discloses an isolated adipose protectant which comprises normal saline, DMEM solution, L-glutamine, penicillin and streptomycin according to the proportion of 500ml to 500ml to (146.1mg-584.4mg) to 125000U to 125000U. The invention further discloses a preparation method and application of the protectant. The protectant has effect of effectively reducing adipose tissue culture stem cell contamination rate. Growth rate of the stem cells during culture of the adipose tissue is higher than that of stem cells growing without protective liquid, and culture time of primary cells can be shortened, culture efficiency is improved, and cost is saved.

Description

technical field [0001] The invention relates to a composition for protecting adipose tissue, a preparation method and application thereof. Background technique [0002] Human adipose tissue contains a large number of adipose tissue-derived cells (ADSC), which are mesenchymal cells that can self-renew, self-replicate, and have multi-directional differentiation potential. Under different inducing factors in vivo and in vitro ADSC can differentiate into cells of various tissues such as adipocytes, nerve cells, chondrocytes, and pancreatic islet cells. At the same time, ADSCs can secrete a variety of pro-angiogenic factors and anti-apoptotic factors, and have great application potential. [0003] In 2001, through liposuction, Zuk et al. isolated for the first time multidirectionally differentiated stem cells, namely adipose stem cells, from the aspirated human adipose tissue. The advantages of ADSCs are: ①The isolation method is simple and operable; ②ADSCs are high in adipose t...

Claims

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Application Information

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IPC IPC(8): C12N5/0775A01N1/02
CPCA01N1/0215A01N1/0226C12N5/0667C12N2500/33
Inventor 赵明建钟伟兴
Owner 中科领康(广州)医疗有限公司
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