Composition for protecting adipose tissue and preparation method and application of composition
A technology for adipose tissue and use, applied in the field of compositions for protecting adipose tissue, can solve the problems of loss of experimental reagent consumables, failure of ADSC culture, high time and economic cost, etc., and achieves the maintenance of traits and differentiation potential, good market application value, and shortened The effect of incubation time
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0059] Embodiment 1 Preparation of protective agent of the present invention and its protective effect for isolated fat
[0060] One, prepare protective agent of the present invention to preserve adipose tissue
[0061] For the formula shown in the table below, dissolve penicillin and streptomycin in sodium chloride injection under sterile conditions, then add L-glutamine to dissolve it, then add DMEM solution, mix the mixture evenly, and then Aseptically dispense into sterilized collection bottles, 30ml per bottle, and add 30ml of collected adipose tissue.
[0062] The formula of protecting agent of the present invention
[0063]
[0064] In the control group, 30ml of adipose tissue was preserved without protective agent.
[0065] 2. Antibacterial experiment of protective agent
[0066] The micro-broth method was used for the determination of the antibacterial effect, and the maximum concentration of the test drug was 50 μg (U) / ml, which was half of the actual drug conc...
Embodiment 2
[0127] Example 2 Various performance tests after the adipose tissue protected by the protective agent of the present invention is used for culturing cells
[0128] One, prepare protective agent of the present invention
[0129] According to the preparation method of Example 1, Group 2, penicillin and streptomycin were dissolved in sodium chloride injection under aseptic conditions, then L-glutamine was added to dissolve them, and then DMEM solution was added. Mix the mixture evenly and make it into a mixed solution containing 125000U of penicillin, 125000U of streptomycin, 365.3mg of L-glutamine, 500ml of DMEM solution and 500ml of sodium chloride injection per liter, and then aseptically dispense it until it is sterilized. Bacteria collection bottle, 30ml per bottle.
[0130] 2. Adipose stem cell growth experiment
[0131] ⑴Experimental method:
[0132] Adipose tissue preserved in sterile collection bottles with protective agent was used as group A, and adipose tissue pres...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com