The invention discloses a preparation method for a biological
chip for detecting food-borne
pathogenic bacteria and belongs to the technical field of food-borne
pathogenic microorganism detection. According to the invention, the unique
protein gene sequence of food-borne
pathogenic bacteria is utilized to design the specific CP, DP and RCP thereof. CP and DP both contain the sequences complementary with the specific sequence of to-be-detected bacterium
genome DNA; the RCP contains no to-be-detected bacterium
genome DNA sequences; the CP is fixed on a formyl-modified
chip surface in a sample application mode, a capturing probe micro-array is prepared, on-
chip CP and DP are annealed and connected with the to-be-detected bacterium
genome DNA,
streptavidin is added and is combined with a
solid-phase RCA amplification product and
elution is performed under a certain condition; a
near infrared fluorescence imager is used for scanning the chip and is used for detecting if CP and DP are connected with each other and if the rolling circle amplification and target detection can be realized, so that the target food-borne
pathogenic bacteria can be specifically detected. The method can be used for detecting various food-borne pathogenic microorganisms and is suitable for high-flux, quick and accurate detection for various food-borne pathogenic microorganisms in food, breeding, ports, and the like.