Silver sulfide quantum dot-chitosan nanometer complex preparation method

A technology of chitosan nano and quantum dots, which is applied in the interdisciplinary fields of chemistry, biology and materials, to achieve the effects of good biocompatibility, high stability and simple preparation methods

Inactive Publication Date: 2013-07-31
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no relevant patent report on the preparation of nanocomposites with the above functions by combining silver sulfide quantum dots with chitosan or chitosan derivatives

Method used

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  • Silver sulfide quantum dot-chitosan nanometer complex preparation method

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Effect test

Embodiment 1

[0023] At room temperature, molecular weight is 100,000, the chitosan that deacetylation degree is 60% is dissolved in deionized water, and the obtained concentration is the chitosan aqueous solution of 5mg / ml; Add ethylenediaminetetraacetic acid and silver nitrate in sequence, the mass ratio of ethylenediaminetetraacetic acid and chitosan is 1:50, and the mass ratio of silver nitrate and chitosan is 1:20. After stirring and reacting for 10 minutes, add potassium sulfide and mercaptopropionic acid, and stir and react for 8 hours, wherein the mol ratio of potassium sulfide and silver nitrate is 1: 2.2, and the mass ratio of mercaptopropionic acid and chitosan is 1: 30; The product was dialyzed with water, centrifuged, washed successively with anhydrous methanol and ether, and vacuum-dried at room temperature to obtain the silver sulfide quantum dot-chitosan nanocomposite. Transmission electron micrographs of the nanocomposite figure 1 shown.

Embodiment 2

[0025] At room temperature, molecular weight is 80,000, carboxymethyl chitosan that deacetylation degree is 50% is dissolved in deionized water, and the obtained concentration is the chitosan aqueous solution of 7mg / ml; Add ethylenediaminetetraacetic acid and silver fluoride successively in the aqueous solution of base chitosan, the mass ratio of ethylenediaminetetraacetic acid and carboxymethyl chitosan is 1:40, the mass ratio of silver fluoride and chitosan is 1 : 18. After stirring and reacting for 15 minutes, add potassium sulfide and thioglycolic acid, and stir and react for 8 hours, wherein the mol ratio of potassium sulfide and silver fluoride is 1: 2.1, and the mass ratio of thioglycolic acid and chitosan is 1: 30; Water dialysis, centrifugation, successive washing with anhydrous methanol and ether, and vacuum drying at room temperature to obtain the silver sulfide quantum dot-carboxymethyl chitosan nanocomposite.

Embodiment 3

[0027] At room temperature, molecular weight is 120,000, the chitosan that deacetylation degree is 80% is dissolved in deionized water, and the obtained concentration is the chitosan aqueous solution of 6mg / ml; Add ethylenediaminetetramethylenephosphonic acid and silver nitrate in sequence, the mass ratio of ethylenediaminetetramethylenephosphonic acid to chitosan is 1:45, and the mass ratio of silver nitrate to chitosan is 1:20. After stirring and reacting for 15 minutes, add sodium sulfide and bovine serum albumin, and stir and react for 10 hours, wherein the mol ratio of sodium sulfide and silver nitrate is 1: 2.5, and the mass ratio of bovine serum albumin and chitosan is 1: 20; Dialyze with water, centrifuge, wash with anhydrous methanol and ether successively, and vacuum dry at room temperature to obtain silver sulfide quantum dot-chitosan nanocomposite.

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Abstract

The present invention relates to a silver sulfide quantum dot-chitosan nanometer complex preparation method, which comprises: 1, dissolving a certain amount of chitosan or a derivative thereof in deionized water at a room temperature to prepare a chitosan aqueous solution with a concentration of 1-20 mg/mL or a chitosan derivative aqueous solution with a concentration of 1-20 mg/mL; 2, under a room temperature stirring condition, sequentially adding a chelating agent and a water soluble silver salt to the chitosan aqueous solution or the chitosan derivative aqueous solution, and carrying out a stirring reaction for 5-20 min; and 3, adding an alkali metal sulfide and a carboxyl compound to the product obtained from the step 2, carrying out a stirring reaction for 5-20 h, carrying out water dialysis and centrifugation on the obtained reaction product, washing sequentially by using absolute methanol and diethyl ether, and carrying out vacuum drying at a room temperature to obtain the silver sulfide quantum dot-chitosan nanometer complex. Compared with the nanometer complex in the prior art, the nanometer complex of the present invention has the following characteristics that: a nanometer complex with a near-infrared fluorescence imaging function is prepared by using a simple and easy-performing method, can be used for biological fluorescence imaging or as a fluorescent probe, and has characteristics of good biocompatibility, no physiological toxicity, and wide application prospects in biomedical fields.

Description

technical field [0001] The invention belongs to the cross field of chemistry, biology and materials, and in particular relates to a silver sulfide quantum dot-chitosan nanocomposite with near-infrared fluorescence imaging function and a preparation method thereof. Background technique [0002] Chitosan is a deacetylated derivative of natural biopolysaccharide chitin, which has free amino groups, very active physical and chemical properties, and has the characteristics of no toxic side effects, good biocompatibility, controllable biodegradation, and non-antigenicity. Therefore, chitosan has been widely used in biomedicine, tissue engineering, sustained release of drugs, immobilization of cells and enzymes, and many other aspects. In recent years, great progress has been made in the research of chitosan as a functionalized nanocarrier. Various ions, molecules and other components have been loaded into the chitosan gel network one after another to form a functionally modified c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L5/08C08K3/30C09K11/58
Inventor 万锕俊谭连江李慧丽
Owner SHANGHAI JIAO TONG UNIV
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