50 results about "In vivo fluorescence" patented technology

The invention relates to systems and methods for tomographic imaging in diffuse media employing a fast reconstruction technique. A hybrid Fourier approach is presented that enables the fast tomographic reconstruction of large datasets. In certain embodiments, the invention features methods of in vivo fluorescence molecular tomographic (FMT) reconstruction of signals, reporters and / or agents (i.e., contrast agents or probes) in a diffusive medium (e.g., a mammalian subject). The method preserves the three-dimensional fluorophore distribution and quantitative nature of the FMT approach while substantially accelerating its computation speed, allowing FMT imaging of larger anatomies.

The present invention relates to a fluorescent probe which includes: a carrier molecule; and fluorescent dye a and fluorescent dye b which are bonded to the carrier molecule. Fluorescent dye a and fluorescent dye b have different excitation wavelengths. Fluorescence resonance energy transfer (FRET) does not occur between fluorescent dye a and fluorescent dye b. The present invention also relates to a fluorescence detection method which includes: a step in which target cells are labelled with the fluorescent probe; and a step in which the target cells labelled with the fluorescent probe are irradiated with excitation light, and fluorescence from the fluorescent probe is observed. Furthermore, the present invention relates to a method for using the fluorescent probe, said method including: astep in which cells are fluorescence labelled using the fluorescent probe; a step in which the fluorescence-labelled cells are screened using a flow cytometer or a fluorescence microscope; a step inwhich the screened fluorescence-labelled cells are transplanted into a living organism; and a step in which the fluorescence-labelled cells in the living organism are observed using an in vivo fluorescence imaging device.

The invention discloses a fluorescence detector. The fluorescence detector comprises a laser beam combining module, a confocal scanning module, a spectral imaging module, a fluorescence microscope and a control display module. The laser beam combining module comprises two near-infrared light continuous lasers, a laser collimating lens, a reflecting mirror and a beam combining lens. The confocal scanning module comprises three switchable dichroic mirrors, three optical filters, a scanning galvanometer, a scanning lens, a pinhole lens and pinholes. The spectral imaging module comprises a beam splitter prism, a focus lens, three slits and three photomultipliers. The fluorescence microscope comprises a total reflection prism, a barrel lens, a micro-objective, a nanometer displacement platform and a sample platform. The control display module is used for controlling the laser, the scanning galvanometer, the micro-objective and the nanometer displacement platform and displaying the image. The fluorescence detector is capable of overcoming the disadvantage of low detection depth of the prior art, realizing the centimeter-level fluorescence detection and simultaneously realizing the fluorescence positioning and component analysis, and the fluorescence detector has broad application prospect in in-vivo fluorescence detection field.

The invention relates to a minimally invasive multiple channel in vivo fluorescence signal real-time detection system, which belongs to the technical field of in-vivo animal fluorescence detection. The system is characterized in that fiber optic bundles, a fiber optic bundle fixing device, a microscopic objective, a dichroic mirror forming a 45 degree angle with the horizontal plane, a fluorescent filter and an optical detector, which are horizontally arranged in sequence, share an axle to form a fluorescence emission light path; an excitation light source, an excitation light filter and a lens are vertically arranged to form an excitation light path; the excitation light forms a 45 degree angle with the dichroic mirror, and is orthogonal with the fluorescence emission light at the centerof the dichroic mirror. The system can detect the concentrations of the fluorescence targets of organs or tissues of in-vivo animals in a minimally invasive, multiple channel, quantitative and continuous way.