Minimally invasive multiple channel in vivo fluorescence signal real-time detection system and method

Abstract

The invention relates to a minimally invasive multiple channel in vivo fluorescence signal real-time detection system, which belongs to the technical field of in-vivo animal fluorescence detection. The system is characterized in that fiber optic bundles, a fiber optic bundle fixing device, a microscopic objective, a dichroic mirror forming a 45 degree angle with the horizontal plane, a fluorescent filter and an optical detector, which are horizontally arranged in sequence, share an axle to form a fluorescence emission light path; an excitation light source, an excitation light filter and a lens are vertically arranged to form an excitation light path; the excitation light forms a 45 degree angle with the dichroic mirror, and is orthogonal with the fluorescence emission light at the center of the dichroic mirror. The system can detect the concentrations of the fluorescence targets of organs or tissues of in-vivo animals in a minimally invasive, multiple channel, quantitative and continuous way.

Description

Technical field: [0001] The invention relates to the field of fluorescence signal detection. It specifically relates to a system and method for detecting fluorescent signals in living animals through a minimally invasive multi-channel fluorescent signal real-time detection system. Background technique: [0002] Usually, in order to detect the concentration change of a certain drug in a certain organ in an animal, or to stage a tumor, it is necessary to collect blood or tissue samples in an organ at intervals for in vitro observation or detection. In recent years, with the widespread application of fluorescent molecular probes, fluorescent molecular probes are used to label drug molecules or tumor cells, and the distribution of probes in animals can be obtained in vitro by techniques such as fluorescent molecular tomography. [0003] Obtaining samples for in vitro observation and detection: relatively accurate quantitative measurements can be achieved, but obtaining samples ...

AI Technical Summary

Problems solved by technology

[0003] Obtaining samples for in vitro observation and detection: relatively accurate quantitative measurements can be achieved, but obtaining samples from animals cannot be "real-time" and "continuous", and there is usually a certain limit to the number of samples taken from the same animal, otherwise it will be cause animal death

[0004] Fluorescent molecular tomography: It can non-invasively detect drug molecules or tumor cells labeled with fluorescent molecular probes in animals. However, since the detected fluorescent signals have been optically scattered by animal tissues, the location and concentration of the probes cannot be determined. The calculation will cause a certain deviation, so the detection by this method cannot be accurately quantified

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