Fluorescence detector

Abstract

The invention discloses a fluorescence detector. The fluorescence detector comprises a laser beam combining module, a confocal scanning module, a spectral imaging module, a fluorescence microscope and a control display module. The laser beam combining module comprises two near-infrared light continuous lasers, a laser collimating lens, a reflecting mirror and a beam combining lens. The confocal scanning module comprises three switchable dichroic mirrors, three optical filters, a scanning galvanometer, a scanning lens, a pinhole lens and pinholes. The spectral imaging module comprises a beam splitter prism, a focus lens, three slits and three photomultipliers. The fluorescence microscope comprises a total reflection prism, a barrel lens, a micro-objective, a nanometer displacement platform and a sample platform. The control display module is used for controlling the laser, the scanning galvanometer, the micro-objective and the nanometer displacement platform and displaying the image. The fluorescence detector is capable of overcoming the disadvantage of low detection depth of the prior art, realizing the centimeter-level fluorescence detection and simultaneously realizing the fluorescence positioning and component analysis, and the fluorescence detector has broad application prospect in in-vivo fluorescence detection field.

Description

technical field [0001] The invention relates to the application of spectrum technology and the technical fields of biomedical engineering, in particular to a fluorescence detector used for fluorescence detection of small animals living bodies. Background technique [0002] With the development of bioluminescence imaging technology, more and more fluorescence detection instruments are used in the fields of food safety, industrial detection, drug research and development, and disease diagnosis. Due to the limitation of the penetration depth of fluorescence, most of the current fluorescence detection instruments image the surface of the tested sample. For example, confocal microscopy, which is widely used in fluorescence detection, can perform high-resolution three-dimensional slice imaging of the tested sample, and at the same time, combined with spectral imaging, it can perform sample composition analysis, but the detection depth is generally not more than 200 microns. [00...

AI Technical Summary

Problems solved by technology

At present, two-photon and multi-photon microscopes herald the development direction of deep tissue biofluorescence detection, but the high cost of ultrafast pulsed near-infrared continuous lasers in these detection systems greatly limits its application range; It is still in the visible light range, which limits the detection depth, so it is still difficult to meet the needs of living fluorescence detection

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