94 results about "Grape seed proanthocyanidin" patented technology

The invention provides a grape seed proanthocyanidins health beverage and a preparation method. The beverage is prepared by the following steps of: extracting leftover grape seeds in a wine plant, performing combined membrane separation technology on the extract to obtain clarified, deastringented and proanthocyanidins-enriched purified liquid, and adding water and various flavoring agents into the purified liquid in a ratio to obtain the product. The beverage does not have the astringent taste of the grape seed per se and yeast taste generated in the wine fermentation process, and also fully keeps the medicinal values of the grape seed.

The invention belongs to the technical field of comprehensive utilization of grape seeds, and concretely relates to a high-efficiency extraction and separation method of grape seed proanthocyanidin oligomer. The high-efficiency extraction and separation method of grape seed proanthocyanidin oligomer comprises the following steps: 1, low-temperature drying and crushing; 2, degreasing; 3, enzyme treatment; 4, proanthocyanidin extraction; 5, low-temperature concentration; 6, proanthocyanidin high polymer degradation; 7, purification; and 8, low-temperature concentration and drying. The extraction and separation method obviously improves the yield of grape seed proanthocyanidins and the content of proanthocyanidin oligomer in an extract liquid, the bioactivity of the proanthocyanidins is well reserved through low-temperature extraction and separation, and the highly pure proanthocyanidin oligomer is obtained through column chromatography grading purification.

The invention provides a preparation method for a grape seed proanthocyanidin extract. The preparation method comprises the following steps: firstly, preparing trichoderma koningii and aspergillus niger direct vat set; and secondly, adding water into grape seeds for grinding; adding glucose for mixing, sterilizing at high temperature, inoculating the bacteria and fermenting, centrifugally removing slag, carrying out ultrafiltration extraction, drying and other processes to obtain the grape seed proanthocyanidin extract.

The present invention belongs to the field of food producing technology, and is especially production process of edible oil added with micro emulsion of grape seed protocyanidin. W / O type g micro emulsion of grape seed protocyanidin is prepared with polyglycerol monooleate as surfactant, alcohol as co-surfactant and edible oil as the continuous phase. When the ratio between surfactant and co-surfactant is 4 to 1, may be reached the maximum grape seed protocyanidin adding amount of 538 mcg / ml, average particle diameter of the dispersed phase particles of 23.96 nm and clear transparent micro emulsion with strong oil oxidation resistance. The present invention adds grape seed protocyanidin as natural antioxidant into edible oil and makes the edible safe, reliable, nutritive and antioxidant.

The invention relates to the technical field of natural skin care products and production methods thereof, and more particularly relates to a grape seed compounded horse oil skin care product and a preparation method thereof. The grape seed compounded horse oil skin care product comprises the following raw materials in percentage by weight: 10%-13% of grape seed oil, 5%-7% of horse oil, 2%-3% of glyceryl monostearate, 0.5%-2% of hexadecanol, 8%-10% of glycerol, 1%-3% of triethanolamine, 1%-3% of tween-80, 0.2%-0.5% of xanthan gum, 0.2%-0.5% of grape seed procyanidine and 0.2%-0.3% of essence. The production method provided by the invention is simple and convenient in process and a safe and high-quality natural skin care product suitable for industrial production; and as the grape seed oil and the horse oil are taken as raw materials, the grape seed compounded horse oil skin care product has the advantages of moisturizing and smoothing skin, enabling skin to have high resilience, delaying senescence and preventing formation of acnes, freckles and the like.

The invention relates to an extraction method of procyanidine in grape seeds and overcomes the problems that procyanidine in grape seed husk is difficultly dissolved out and the polymerization degreeof the procyanidine in the extract is high are overcome. According to the extraction method of the procyanidine in the grape seeds, aspergillus niger is used as strain for fermenting the grape seeds;the enzymes such as cellulase and pectinase produced by the aspergillus niger are used for degrading the grape seed husk, so that the procyanidine is more easily dissolved out; alcohols with differentvolume fractions are used as desorption solutions for eluting macroporous adsorption resin; the procyanidine is graded and is subsequently subjected to partially acidolysis, so that the content of procyanidine oligomer in the extract is increased. The content of the oligomerized procyanidine in the extract is greater than or equal to 98%; the average polymerization degree of the procyanidine is 2.5-3.0; the procyanidine is easily absorbed in the body and is high in anti-oxidation activity.

The invention discloses an immunity-improving health product. The immunity-improving health product is made of, by weight, 10-20% of dendrobium officinale powder, 20-40% of grape seed proanthocyanidin extract, 1-5% of total saponins of panax ginseng, 1-3% of selenium carrageenan, and 40-60% of starch. A formula of the immunity-improving health product is simple. The immunity-improving health product is convenient to eat. Functions of antioxidation, removal of radicals and cardiovascular protection are enhanced. The immunity-improving health product is anti-aging and cancer-inhibiting, and further has functions of promoting blood circulation to remove blood stasis and well preventing 'high blood pressure, high blood sugar and high blood lipid'. The immunity-improving health product can be consumed to effectively improve human immunity, and is safe, reliable and evidently effective.

The invention provides fruit vinegar capsules and a preparation method applied in the technical field of food. A product formulation comprises fruit vinegar powder, rice bran dietary fiber, inulin, soy peptide, barley seedling leaf powder, lotus leaf powder and grape pip proanthocyanidins. The preparation method comprises: taking high-quality fresh fruits or concentrate juice thereof as main raw material; obtaining fruit vinegar through alcohol microbial fermentation and acetic acid microbial fermentation; concentrating the fruit vinegar through membrane concentration, spray-drying and grinding the obtained product to prepare the fruit vinegar into titratable total acid; washing barley seedling leaves and lotus leaves clean respectively and then preparing the two into dry powder after drying, grinding and sieving; weighing rice bran dietary fiber, inulin, soy peptide and grape pip proanthocyanidins according to the weight in the formulation; mixing the materials well by use of a high-speed mixer; drying and sterilizing the mixture under vacuum through microwave; and obtaining the fruit vinegar capsules through the filling of an full-automatic capsule filling machine. The preparation method has the advantage of novel scientific conception, and the capsules prepared by concentrating and drying the fruit vinegar are safe, effective, incapable of causing gastrointestinal discomfort or dependency, suitable for long-term eating and capable of rapidly clearing free radicals and superfluous fat in human bodies, neutralizing acidic constitution and increasing satiety so as to achieve the aim of losing weight.

The invention relates to a preparation method of oligomer grape seed procyanidin. The preparation method comprises the following steps that: firstly, after grape seeds are washed and cleaned by clear water, the grape seeds are added into an ethanol solution, leached, filtered and removed, and an extracting solution is collected; secondly, the extracting solution is regulated by an NaOH solution, slightly stirred to separate out a brick-red procyanidin coarse sediment, and the sediment is filtered and collected; thirdly, the sediment is put into the ethanol solution and dissolved, and filter liquor containing dissolved procyanidin is collected; and finally, the filter liquor is filtered by a nano filter film, oligomer procyanidin intercepted and deposited on the filter film is collected, the sediment is respectively washed by acetone and ethanol, and an oligomer procyanidin product is obtained after the sediment is put into a drying oven and dried.

The invention relates to the technical field of cosmetics, and specifically relates to a preparation method of grape seed procyanidin sun cream. Aiming at the characteristic of a plant sun cream, namely a grape seed procyanidin extract, the biological activity of procyanidin as an effective component is maintained to the maximum extent by adopting appropriate adding forms and adding methods. The technical scheme adopted by the invention comprises the following operational steps: I, screening grape brewing dregs, separating and sorting grape seeds with qualified quality, and crushing and degreasing to obtain grape seed degreased powder; II, preparing the grape seed procyanidin extract; III, preparing the sun cream; IV, packaging.

A method for extracting grape seed oil and proanthocyanidins, involving a method of extracting active ingredients from natural products, soaking crushed grape seeds in a mixed solvent to obtain grape seed oil extract and proanthocyanidin extracts, extracting grape seed oil extracts and proanthocyanidins After liquid separation, the proanthocyanidin extract is purified by an ultrafiltration membrane to obtain proanthocyanidin, and the grape seed oil extract is evaporated to remove the solvent to obtain grape seed oil. The grape seed oil and proanthocyanidins in the grape seeds can be fully extracted by using the above method, the extraction method is simple, the equipment cost is low, the extracted grape seed oil and proanthocyanidins are high in purity, and the production cost is low. The yield of the prepared proanthocyanidins is as high as 86.7%, and the purity is higher than 97.8%. The extraction time is short, and a high extraction rate can be quickly completed to obtain a high-purity product. It is suitable for use by various small and medium-sized enterprises, and it is also suitable for wine production enterprises to recycle and process grape seed leftovers by themselves.

The invention belongs to the technical field of drink processing and specifically relates to a healthcare drink capable of improving immunity and a preparation method thereof. The preparation method comprises the following steps: placing red dates, brown sugar, dandelion, pawpaw, American ginseng and astragalus into a ceramic container, heating, cooling and filtering to obtain liquor A; adding grape seed procyanidin, ganoderan, lentinan, pine pollen disruption powder, algal polysaccharide and cordyceps militaris powder into the liquor A, heating, and then cooling to the normal temperature to obtain liquor B; and adding a sweetening agent into the liquor B, homogenizing, uniformly mixing, carrying out pasteurization and filling. The healthcare drink has the beneficial effects that if people drink the healthcare drink prepared from the various raw materials according to the formula, the immunity of the organism can be obviously improved, the organism can be protected from being invaded and harassed by external germs, and body health can be protected.

A compound propolis preparation for resisting to cancer, inflammation and virus is prepared from propolis, notoginseng, schisandra fruit, dandelion herb, ganoderma polyose, grape seed's proanthocyanidin, and wall-broken ganoderma spore powder through supercritical CO2 extraction of propolis, preparing microsoftgels, and mixing with others. Its advantage is high curative effect.

The present invention discloses a method for improving the water solubility of grape seed proanthocyanidin. The method comprises the following contents of: carrying out reflux extraction of grape seeds by deionized water having a temperature of 85 to 95 DEG C, and filtering to obtain crude extract solution; adding pectase, amylase, chitosan, or high-purity ethanol into the crude extract solution and stirring slightly, and standing when flocky deposition occurs; ultrafiltrating supernatant liquor again; and concentrating and drying to obtain water soluble grape seed proanthocyanidin product.

The present invention discloses a grape seed proanthocyanidin additive and a preparation method and an application thereof. The grape seed proanthocyanidin additive is prepared by evenly mixing grape seed proanthocyanidins, corn starch and rice hull powder at a weight ratio of (3-15) : 1 : 2. In the production practice of livestock breeding industry and especially in the processing of ruminant feed, the grape seed proanthocyanidin additive is added, which can significantly reduce the generation amounts of rumen fermentation carbon dioxide and hydrogen up to 34% and 78% and thus effectively reduce methane emissions up to 45%. The grape seed proanthocyanidin additive is simple in preparation method, strong in operability and suitable for the breeding practical application of ruminate animals of dairy cattle, beef cattle, mutton sheep, etc., can significantly inhibit the methane greenhouse gas emissions of the ruminant animals, and have very important significances for improving feed energy utilization efficiency, saving energy and reducing emission, and controlling the greenhouse effects.

The invention provides a preparation method of grape seed procyanidine sustained-release microcapsules, which comprises the following steps: uniformly mixing a sodium alginate water solution and a hydroxypropyl methyl cellulose water solution to obtain a mixed solution; uniformly mixing the grape seed procyanidine aqueous solution with the mixed solution to obtain a sodium alginate-hydroxypropyl methyl cellulose-grape seed procyanidine mixed solution; and injecting the solution into the microcapsule stationary liquid to obtain the grape seed procyanidine sustained-release microcapsule. According to the preparation method, grape seed procyanidine is used as a core material, and the grape seed procyanidine is embedded by utilizing the characteristic that sodium alginate is in contact with calcium ions and can be instantaneously gelatinized; the operation steps are simple without adding an organic solvent; by utilizing the characteristic of pH sensitivity of hydroxypropyl methyl cellulose, the stability and embedding rate of the microcapsule are improved, and the release speed of the embedded grape seed procyanidine can be adjusted; and the prepared grape seed procyanidine sustained-release microcapsule can be applied to the fields of health-care products and functional foods.

The invention relates to a feed additive capable of relieving endoplasmic reticulum stress of jewfishes as well as a preparation method and application of the feed additive, and belongs to the field of feeds. Each 100 parts by weight of the feed additive comprises 20-30 parts by weight of alfalfa sapinogenin, 10-20 parts by weight of chelated zinc methionine, 5-15 parts by weight of taurine, 20-30parts by weight of radix puerariae powder, 5-10 parts by weight of grape seed proanthocyanidins, and the balance of a carrier. The raw materials of the feed additive are safe and free from harmful remaining, and create conditions for production of a green feed and aquatic products. The preparation method of the feed additive comprises the following steps of mixing the raw materials in parts by weight. The preparation method is simple, good in condition controllability and good in stability, and has favorable application prospects. The feed additive is applied to preparation of a jewfish feed,the antioxidant ability of livers of the jewfishes can be improved, the oxidative damage of endoplasmic reticulum can be reduced, the endoplasmic reticulum stress protein expression can be reduced, the endoplasmic reticulum stress can be relieved, and the endoplasmic reticulum can be maintained stable.

The invention relates to a composite capsule for delaying senescence, which aims at solving the technical problems of making up the blank of the prior art and providing a composite capsule for delaying senescence. The composite capsule for delaying senescence internally contains the following ingredients in parts by weight: 245-300 parts of soybean extractive, 45-55 parts of grape seed extractive, 1.35-1.65 parts of vitamin B1, 1.35-1.65 parts of vitamin B6 and 0.0027-0.0033 part of vitamin B12. According to the composite capsule, the soybean isoflavone, grape seed procyanidine and B-group vitamins are mutually prepared and acted cooperatively, various essential nutrients are provided and at the same time the conditioning of the soybean isoflavone to body functions of the middle-aged and the elderly is brought into play; if the product is taken for a long time, the health-care effects of delay senescence and improving climacteric syndrome are achieved, and therefore, the composite capsule is applicable to the vast middle-aged and the elderly.

The invention aims at providing a scheme capable of reversing the medicine resistance fundamentally, improving the curative effects of chemotherapy medicines and reducing the toxic and side effects ofthe chemotherapy medicines, and particularly provides application of grape seed procyanidine in preparation of a combination medicine in a cancer chemotherapy scheme. The combination medicine is usedfor being cooperatively used with the chemotherapy medicines used for treating cancers, and therefore the phenomenon is reduced that due to the medicine resistance exists, the effects of the chemotherapy medicines are reduced, or the risk of toxic and side effects occurring when the dosage of the chemotherapy medicines is increased for reducing the medicine resistance is reduced.

The invention relates to a method for enhancing biological and protein stability of iced white wine, which comprises the following steps: (1) filtering Italian Riesling iced white wine subjected to alcohol fermentation through a 0.45 mu m filter membrane to obtain the filtered Italian Riesling iced white wine; (2) preparing an iced wine solution containing lysozyme and an iced wine solution containing sodium metabisulfite; and (3) sequentially adding the iced wine solution containing lysozyme, iced wine solution containing sodium metabisulfite and a grape seed proanthocyanidin concentrated solution into 1L of filtered Italian Riesling iced white wine, and regulating the pH value to 3.5-4.5 with a saturated potassium bitartrate solution, wherein the lysozyme concentration is 150-250 mg / L, the total SO2 concentration is 150-200 mg / L, and the total polyphenol concentration of the wine is 0.5-0.7 g / L. The method can prevent the iced white wine from the pollution of putrefactive microorganisms in the iced wine storage period without influencing the protein stability of the Italian Riesling iced white wine, and has the characteristics of no pollution, high safety and no residue as compared with antibiotics and chemical preservatives.

The invention discloses an oral medicine composition for resisting radiation, preventing dental caries and diminishing inflammation and a preparation thereof, and belongs to the technical field of oral medicine preparations. The composition is prepared from the following components in parts by mass: 100-500 parts of white hyacinth bean extract, 100-2000 parts of grape seed proanthocyanidin extract, 300-3000 parts of tea polyphenol, 0.01-0.1 part of epidermal growth factor EGF, 10-200 parts of triclosan and 2-6 parts of ammonium hexafluorosilicate. The oral medicine composition and the preparation thereof disclosed by the invention have a durable antibacterial effect, can be attached to the surface of teeth or oral cavity for a long time to form a protective film, can be used for tenderly protecting the oral cavity, has an excellent taste, and is safe to human bodies.

The invention discloses a compound feed additive with an antioxidant function capable of relieving mycotoxicosis of a piggy. The compound feed additive comprises the following components in percentage by weight: 1-2% of vitamin A, 3.23-4% of vitamin C, 5.11-6% of vitamin E, 3-4% of selenium yeast, 2-3% of grape seed proanthocyanidins and 81-85% of mixture of rice chaff and husks. The compound feed additive disclosed by the invention aims at a toxic mechanism of mycotoxin, selects a natural antioxidant as the raw material, adopts a reasonable compounding ratio of the components and has better relieving effects on oxidative injuries, structural damage and growth inhibition, which are caused by the mycotoxin, of intestinal tracts of an animal; besides, the compound feed additive with the antioxidant function, disclosed by the invention, has a better relieving effect on various animal toxic symptoms caused by the mycotoxin in the practice production; the components of the additive disclosed by the invention have the characteristics of safety and nutrition, micro nutritional substances in the feed cannot be adsorbed, and the breeding efficiency can be improved.

The invention discloses a method for preparing grape seed proanthocyanidin extract. The method comprises the following steps: grinding grape seeds, degreasing with petroleum ether, drying, adding intoa triangular flask, adding a buffer solution, performing enzymolysis for 1.5-2.5 hours, rapidly raising the temperature, fully inactivating, performing centrifugal separation, preserving the supernatant, adding an ethanol solution having the volume fraction of 65-75% into a water bath for 45-55 minutes, performing suction filtration, performing reduced pressure distillation, and performing freezedrying on the concentrate so as to obtain the crude GSPE; adding an AB-8 macroporous resin into a conical flask, adding an aqueous solution of the crude GSPE, performing oscillating adsorption into atable for 47-49 hours, filtering, pouring the resin washed with distilled water into ethanol having the volume fraction of 50-60% for desorbing for 25-27 hours, filtering the desorbed solution, performing reduced pressure distillation, and performing freeze drying, thereby obtaining the product. The method is simple, convenient and rapid, a large quantity of grape seed proanthocyanidin extract issuccessfully prepared, and the extract has obvious antioxidant activity and can be used for drug preparation.

The invention relates to a health product namely the Pushenkang health product. The health product is made from effective ingredients and an adjuvant which is acceptable in health products, wherein, the effective ingredients are made from the following bulk drugs by weight percentages: 26-40% of proanthocyanidin of grape seeds, 0.5-1.5% of resveratrol, 2-4% of ophidia black snake, 8-12% of rhodiola root, 8-12% of earth worm, 13-21% of ixeris sonchifolia, 7-11% of kudzuvine root and 13-21% of fruit of hawthorn. The formula combines the advanced international medical theory of resisting oxidation and clearing free radicals with the traditional formula of traditional Chinese medicine to protect organisms and histiocytes and prevent diseases from the source of body. The health product has the functions of preventing and treating coronary heart disease, angina pectoris and myocardial infarction, reducing blood sugar, resisting tumor, allergy, aging, enhancing immunity, beautifying and the like.

The invention discloses an application of GSPE (grape seed proanthocyanidin extract) in preparation of a targeted drug for treating cell calcium overload injury. The cell calcium overload injury means vascular endothelial cell calcium overload injury resulting in pyemia, the GSPE does not influence classic signal channels TLR4 and NFkB of LPS (lipopolysaccharide), Orail signals are specifically blocked, and calcium overload signals are blocked. The application indicates that the GSPE can effectively block activation of Orail mediated SOCE (store-operated calcium entry) by LPS, blocks extracellular calcium ion inflow initiated by the Orail mediated SOCE, reverses lung microvascular endothelial cell calcium overload injury of mice with pyemia and reduces the death rate of the mice with pyemia.

The invention discloses a preparation method of grape seed procyanidin, which comprises the following steps: the grape seeds are extracted in the water solution with the temperature of 85 to 95 DEG C, and the grape seed procyanidin crude extract solution is obtained through filtering; then the crude extract solution is heated, evaporated and concentrated, ethanol is added into the concentrated solution to dissolve the procyanidin and to remove the precipitates so as to obtain the grape seed procyanidin ethanol extract solution; and then the ethanol extract solution is undertaken the adsorption and separation with column chromatography, 30 to 40 percent of ethanol water solution is washed out, the elution liquid is collected, and the elution liquid is dried to obtain the oligomer procyanidin product. The method can improve the efficiency and can reduce the consumption of the solvent and the extraction cost.

The invention discloses a black fungus polysaccharide and grape pip procyanidine composite antioxidant capsule, and relates to an antioxidant product. The black fungus polysaccharide and grape pip procyanidine composite antioxidant capsule is prepared by mixing 100g of starch and 6-12g of black fungus polysaccharide extract and grape pip procyanidine composition, wherein the black fungus polysaccharide extract and grape pip procyanidine composition is prepared from 40-60 parts by weight of black fungus polysaccharide extract and 40-60 parts by weight of procyanidine extract. The preparation method comprises the following steps of: firstly, selecting materials and weighing; then, extracting effective active components; mixing the extract with starch to prepare powder; and finally, packaging according to different specifications. The capsule is simple in production process. In vitro experiments show that the composite capsules have the adding, synergistic interaction and antioxidant effect. The capsules have the characteristics of convenience in administration in form and quick and complete absorption, so that the capsules are suitable for all crowds to take, and have wide market prospect.

The invention relates to a grape-seed specific mean-degree-of-polymerization procyanidine extract and a preparing method and application thereof. According to the grape-seed specific mean-degree-of-polymerization procyanidine extract and the preparing method and application thereof, the specific mean-degree-of-polymerization procyanidin extract with the mean degree of polymerization (mDP) of 4.0-9.0 and the total content of anthocyanin larger than or equal to 50% is obtained from grape seeds through solvent fractional precipitation or resin gradient elution, wherein the specific mean-degree-of-polymerization procyanidin extract is mainly composed of (+)-catechinic acid, (-)-epicatechin and (-)-epicatechin gallate. The specific mean-degree-of-polymerization procyanidin extract can be used for preventing and treating diarrhea caused by multiple factors.

The invention discloses a method for extracting grape seed-peanut shell-chrysanthemum to prepare an antibacterial, anti-inflammatory and anti-oxidization component of toothpaste. The method comprisesthe following steps: drying grape seeds, crushing, sieving and degreasing; adding an organic solvent ethanol into degreased grape seed powder; carrying out ultrasonic extraction and then carrying outmicrowave radiation extraction; centrifuging and collecting supernatant; decompressing and carrying out rotary evaporation; freezing and drying in vacuum; washing peanut shells and drying; drying thepeanut shells into powder through a pulverizer and sieving; adding an acetone water solution into peanut shell powder; refluxing and extracting in a water bath; filtering and collecting filtrate; carrying out rotary evaporation and concentration; making the capacity constant until a product has a semi-extract state, and putting the product into a vacuum drying box; drying to obtain peanut shell antibacterial extract; removing flower buds of dried chrysanthemums, sieving and removing powder; adding water into the chrysanthemums; then adding cellulase and extracting; carrying out rotary evaporation and concentration on an extracting solution; carrying out alcohol precipitation with ethanol to obtain chrysanthemum polysaccharide; and uniformly mixing and compounding the chrysanthemum polysaccharide, grape seed procyanidin and the peanut shell antibacterial extract to prepare the antibacterial, anti-inflammatory and anti-oxidization component of the toothpaste.