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High-efficiency extraction and separation method of grape seed proanthocyanidin oligomer

A technology of proanthocyanidin and separation method, which is applied in the field of efficient extraction and separation of grape seed proanthocyanidin oligomers

Active Publication Date: 2017-02-08
SHANDONG ACAD OF GRAPE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, so far there is no very effective method for the preliminary separation and identification of oligomeric proanthocyanidins in grape seeds.

Method used

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  • High-efficiency extraction and separation method of grape seed proanthocyanidin oligomer
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  • High-efficiency extraction and separation method of grape seed proanthocyanidin oligomer

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Experimental program
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Effect test

Embodiment 1

[0039] The method for efficiently extracting and separating grape seed proanthocyanidin oligomers comprises the following steps:

[0040] (1) Low-temperature drying and crushing: first, the grape seeds are dried at a low temperature at a temperature of 30°C and a vacuum of 0.06Mpa; then crushed to obtain 40 mesh grape seed powder, wherein the grape seeds are unfermented white grape seeds;

[0041] (2) Degreasing: The obtained grape seed powder is extracted with supercritical carbon dioxide to extract grape seed oil to remove grease at low temperature to obtain defatted grape seed powder, wherein the extraction pressure of supercritical carbon dioxide extraction is 32.30 MPa, and the extraction temperature is 41.5 ° C; the extraction time is 75 minutes; the final oil yield was 16.0%, and the residual oil yield was 0.2% to 0.5%.

[0042] (3) Enzyme treatment: use cellulase and β-glucosidase to carry out biological enzyme treatment on the obtained defatted grape seed powder: take t...

Embodiment 2

[0049] The method for efficiently extracting and separating grape seed proanthocyanidin oligomers comprises the following steps:

[0050] (1) Low-temperature drying and crushing: first, the grape seeds are dried at a low temperature at a temperature of 50°C and a vacuum of 0.09 MPa; then crushed to obtain 60-mesh grape seed powder, wherein the grape seeds are unfermented white grape seeds;

[0051] (2) Degreasing: The obtained grape seed powder is extracted with supercritical carbon dioxide to extract grape seed oil to remove grease at low temperature to obtain defatted grape seed powder, wherein the extraction pressure of supercritical carbon dioxide extraction is 32.30 MPa, and the extraction temperature is 41.5 ° C; the extraction time is 75 minutes; the final oil yield was 16.0%, and the residual oil yield was 0.2% to 0.5%.

[0052] (3) Enzyme treatment: use cellulase and β-glucosidase to carry out biological enzyme treatment on the obtained defatted grape seed powder: tak...

Embodiment 3

[0059] The method for efficiently extracting and separating grape seed proanthocyanidin oligomers comprises the following steps:

[0060] (1) Low-temperature drying and crushing: first, the grape seeds are dried at a low temperature at a temperature of 45°C and a vacuum of 0.07 MPa; then crushed to obtain 40-60 mesh grape seed powder, wherein the grape seeds are unfermented white grape seeds;

[0061] (2) Degreasing: The obtained grape seed powder is extracted with supercritical carbon dioxide to extract grape seed oil to remove grease at low temperature to obtain defatted grape seed powder, wherein the extraction pressure of supercritical carbon dioxide extraction is 32.30 MPa, and the extraction temperature is 41.5 ° C; the extraction time is 75 minutes; the final oil yield was 16.0%, and the residual oil yield was 0.2% to 0.5%.

[0062] (3) Enzyme treatment: use cellulase and β-glucosidase to carry out biological enzyme treatment on the obtained defatted grape seed powder: ...

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Abstract

The invention belongs to the technical field of comprehensive utilization of grape seeds, and concretely relates to a high-efficiency extraction and separation method of grape seed proanthocyanidin oligomer. The high-efficiency extraction and separation method of grape seed proanthocyanidin oligomer comprises the following steps: 1, low-temperature drying and crushing; 2, degreasing; 3, enzyme treatment; 4, proanthocyanidin extraction; 5, low-temperature concentration; 6, proanthocyanidin high polymer degradation; 7, purification; and 8, low-temperature concentration and drying. The extraction and separation method obviously improves the yield of grape seed proanthocyanidins and the content of proanthocyanidin oligomer in an extract liquid, the bioactivity of the proanthocyanidins is well reserved through low-temperature extraction and separation, and the highly pure proanthocyanidin oligomer is obtained through column chromatography grading purification.

Description

technical field [0001] The invention belongs to the technical field of comprehensive utilization of grape seeds, and in particular relates to a method for efficiently extracting and separating grape seed proanthocyanidin oligomers. Background technique [0002] Grape seed proanthocyanidins have a C6-C3-C6 structure, and are polyphenolic compounds formed by condensation of C4-C8 or C4-C6 with flavan-3-ol as the basic unit. Among them, the oligomer proanthocyanidins are n=2~ 5. High polymer proanthocyanidin is n>5. [0003] The biochemical activity of proanthocyanidins is manifested in its antioxidant activity and the effect of scavenging free radicals such as superoxide anion and hydroxyl radicals. Free radicals are harmful compounds produced in the oxidation reaction of the body. They have strong oxidative properties and can damage the tissues and cells of the body, causing chronic diseases and aging effects. Studies have shown that proanthocyanidins are effective free ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/16C12P17/18C07D311/62C07G99/00
CPCC12P17/162C12P17/181C07D311/62C07G99/00Y02P20/54
Inventor 高德艳胡文效任继波梁红敏魏彦锋汤晓宏张晶莹
Owner SHANDONG ACAD OF GRAPE
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