Preparation method of oligomer grape seed procyanidin

Abstract

The invention relates to a preparation method of oligomer grape seed procyanidin. The preparation method comprises the following steps that: firstly, after grape seeds are washed and cleaned by clear water, the grape seeds are added into an ethanol solution, leached, filtered and removed, and an extracting solution is collected; secondly, the extracting solution is regulated by an NaOH solution, slightly stirred to separate out a brick-red procyanidin coarse sediment, and the sediment is filtered and collected; thirdly, the sediment is put into the ethanol solution and dissolved, and filter liquor containing dissolved procyanidin is collected; and finally, the filter liquor is filtered by a nano filter film, oligomer procyanidin intercepted and deposited on the filter film is collected, the sediment is respectively washed by acetone and ethanol, and an oligomer procyanidin product is obtained after the sediment is put into a drying oven and dried.

Description

Technical field: [0001] The invention relates to a preparation method of oligomer grape seed proanthocyanidins. The oligomer proanthocyanidins are prepared by ethanol solution extraction, acid-base condition purification, and nanofiltration membrane separation, and belong to the field of extraction of natural active substances in biotechnology. Background technique: [0002] Proanthocyanidins, often called procyanidine or proanthocyanidins abroad, are natural phytochemicals formed by the polymerization of different amounts of catechin or epicatechin monomers. They are widely found in various plants such as oranges, lemons, olives, and grapes. Among them, it has good anti-oxidation and free radical scavenging effects, and has many applications in food, health products, pharmaceuticals and other fields. Among them, grape seeds are easy to preserve and process, and are rich in proanthocyanidins, so in the prior art, grape seeds are mostly used as raw materials to extract proant...

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a new method for separating and purifying oligomer grape seed proanthocyanidins with ultrafiltration membrane and nanofiltration membrane in order to overcome the defects of low oligomer content, complex process and high production cost in the products existing in the current proanthocyanidin extraction method. method, the content of oligomer components can be as high as 60%, which is about double that of similar products at home and abroad