105 results about "Epicatechin gallate" patented technology

The invention described herein encompasses methods and compositions of treating cancer or solid tumors comprising the administration of a therapeutically effective amount of catechins, a group of polyphenols found in green tea, to a mammal in need of such therapy. Compositions of catechins include but not limited to, epigallocatechin gallate (EGCg), epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC). The unique compositions of the invention contain various combinations of the catechins with reduced levels of EGCg, alone or in combination with each other or other therapeutic agents and are used to treat primary and metastatic cancers in humans. The invention also encompasses the varying modes of administration of the therapeutic compounds.

The invention described herein encompasses a methods and compositions of treating cancer or solid tumors comprising the administration of a therapeutically effective amount of catechins, a group of polyphenols found in green tea, to a mammal in need of such therapy. Compositions of catechins include but not limited to, epigallocatechin gallate (EGCg), epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC). The unique compositions of the invention contain various combinations of the catechins, alone or in combination with each other or other therapeutic agents and are used to treat primary and metastatic cancers in humans. The invention also encompasses the varying modes of administration of the therapeutic compounds.

A nutrient pharmaceutical formulation composition useful for treating cancer comprising ascorbic acid, L-lysine, L-proline and at least one polyphenol compound selected from the group consisting of epigallocatechin gallate, epicatechin gallate, epigallocatechin, epicatechin, catechin and the use of the composition in treating cancer and other tumors are provided.

The invention described herein encompasses methods and compositions of preventing or treating cancer comprising the administration of a combination of catechins and proanthocyanadins. Compositions of catechins include but not limited to, epigallocatechin gallate (EGCg), epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC). Compositions of proantho-cyanains include, but are not limited to, blue fruit extracts. The unique compositions of the invention contain various combinations of the catechins and proanthocyanadins, in combination with each other or other therapeutic agents and are used to treat primary and metastatic cancers in humans. The invention also encompasses various modes of administration of the therapeutic compounds, including formulations which may be used as a dietary or nutritional supplement or as a therapeutic compound.

The invention relates to a technology and a method for extracting high-purity Epigallocatechin gallate (EGCG) and Epicatechin-3-gallate (ECG) from green tea. The green tea is common green tea in the market; after combined purification of the green tea through caffeine deposit, liquid-phase extraction and scintillation silica gel column chromatography technology, the high-purity EGCG can be obtained, and the purity of the EGCG after purification can reach more than 97 percent; the concentration of the caffeine deposit is between 20 and 50 mmol / L; a liquid-phase extractant is chloroform and ethyl acetate; filler of a scintillation silica gel chromatographic column is silica gel G (between 200 and 300 meshes), wherein the length of the column is 30 centimeters and the inside diameter is 2.0 centimeters; the ethyl acetate and petroleum ether (between 60 and 90 DEG C) are taken as eluant, and the volume ratio of the ethyl acetate to the petroleum ether is 3 to 2; 0.4 to 0.8 percent of trifluoroacetic acid is added; various reagents are general reagents and are economic and easy to purchase; and except the chloroform, other reagents are safe and nontoxic, and the chloroform is easy to remove. The method can be applied to industrial mass production of the high-purity EGCG and the ECG.

The invention described herein encompasses methods and compositions of preventing or treating cancer comprising the administration of a combination of therapeutically effective amount of catechins, a group of polyphenols found in green tea, and Capsicum extracts. Compositions of catechins include but not limited to, epigallocatechin gallate (EGCg), epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC). The unique compositions of the invention contain various combinations of the catechins and Capsicum extracts, in combination with each other or other therapeutic agents and are used to treat primary and metastatic cancers in humans. The invention also encompasses the varying modes of administration of the therapeutic compounds, including a formulation which may be used as a therapeutic compound for the treatment of cancer or as a dietary supplement for the prevention of cancer.

The invention discloses a method for identifying the quality of Biluochun tea, which comprises the following steps of: after soaking the Biluochun tea, scanning the soaked solution by using an ultraviolet and visible spectrophotometer; and, after leaching and extracting another Biluochun tea, measuring the contents of epigallocatechin, epigallocatechin epicatechol gallate, epicatechin and epicatechin epicatechol gallate in the tea from the leached and extracted solution by adopting a high efficiency liquid chromatography. According to the method disclosed by the invention, a technology for identifying the quality of the Biluochun tea is established by adopting (a technology that spectrum and chromatograph are combined) spectrum and chromatograph analysis; the comprehensive evaluation data of green tea quality is provided; the method has the characteristics of being accurate, reliable, simple, convenient and efficient; and the method can be popularized and applied in the field of the green tea quality counterfeit identifying technology.

The invention described herein encompasses methods and compositions of preventing or treating cancer comprising the administration of a combination of catechins and vanilloids. Compositions of catechins include but not limited to, epigallocatechin gallate (EGCg), epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC). In a preferred embodiment the catechins have been treated with tannase. Compositions of vanilloids include, but are not limited to vanillylamine, the head group of capsaicin. The unique compositions of the invention contain various combinations of the catechins and vanilloids, in combination with each other or other therapeutic agents and are used to treat primary and metastatic cancers in humans. The invention also encompasses various modes of administration of the therapeutic compounds, including formulations which may be used as a dietary or nutritional supplement or as a therapeutic compound.

The invention discloses a method for preparing tea polyphenol rich in methylated catechin from tea cultivated in China. The method comprises the following steps: taking the tea cultivated in China, with higher content of methylated catechin, as a raw material, extracting with hot water, and drying to obtain a crude extract; treating the crude extract with a macroporous resin A to obtain a tea polyphenol component I (with content of methylated catechin being about 18%) rich in methylated catechin; separating and purifying the tea polyphenol component I rich in methylated catechin with a Toyopearl HW-40S resin to obtain monomer components (with purity being more than 95%) of (-)-epigallocatechin3-O-(3-O-methyl) gallate (EGCG3''Me) and (-)-3-O-methyl-epicatechin gallate (ECG3' Me) respectively; and treating the tea polyphenol component I rich in methylated catechin with a macroporous resin B to obtain a tea polyphenol II (with content of methylated catechin being more than 60%) rich in methylated catechin. As a preparation process with the advantages of low cost, high efficiency and environment friendliness, the method is easy to implement industrial production and has broad application prospects.

The invention provides a new tea leaf and a manufacturing method and applications thereof. In the manufacturing method, fresh leaves of a special camellia crassicolumna var. multiplex (Changet Tan) Ming) are taken as the raw material and the tea leaf is manufactured through processing techniques such as deactivation of enzymes, rolling and drying and is characterized by not containing purines alkaloid such as caffeine and theocin; composition and content of tea polyphenol of the tea leaf is similar to the composition and content of the tea leaf made of usually seen tea tree (Camellia sinensis and ribes heterotrichum) and the tea leaf is rich in tea polyphenolic components such as epicatechin (EC), Epicatechin gallate(ECG), epigallocatechin gallate (EGCG) and features obvious oxidation resistance and antibiosis. The tea leaf can be used for qualify tea drinks, functional health care food, cosmetics for special purpose and natural medicine and used as food additive applied to food-processing industry.

Disclosed are compositions for mammalian, avian or piscian reproductive cells and methods for the collection, holding, processing, in vitro fertilization, sexing culturing, or storing (including long-term cryopreservation) of mammalian or avian reproductive sperm cells. The compositions comprise suitable reproductive cell media and antioxidant bioflavonoids. These may be comprised of Oligomeric Proanthocyanidins (OPC) which include the specific molecules of OPCs, that are namely; (epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECG), and epigallocatechin gallate (EGCG)).

The invention described herein encompasses methods and compositions of preventing or treating cancer comprising the administration of a combination of therapeutically effective amount of catechins, a group of polyphenols found in green tea, and Capsicum extracts. Compositions of catechins include but not limited to, epigallocatechin gallate (EGCg), epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC). The unique compositions of the invention contain various combinations of the catechins and Capsicum extracts, in combination with each other or other therapeutic agents and are used to treat primary and metastatic cancers in humans. The invention also encompasses the varying modes of administration of the therapeutic compounds, including a formulation which may be used as a therapeutic compound for the treatment of cancer or as a dietary supplement for the prevention of cancer.

The invention relates to method for using plant extractive catechin to prepare drug, wherein it uses green tea extractive as material; extracts in organic solvent; removes caffeine; uses HPLC to separate the catechin, as epigallocatechin, etc. The inventive drug can be used to treat virus infection of respiratory tract.

The invention discloses a method for preparing catechin monomers, which comprises the following steps of: pretreating water extraction solution of tea or tea tailings; enriching a catechin mixture; adsorbing the catechin mixture by using a reversed-phase chromatograph packing; performing gradient elution; performing gel filtration chromalography on eluent; and concentrating, crystallizing, and drying. The invention also provides catechin monomers of epigallocatechin gallate (EGCG) and epicatechin gallate (ECG) prepared by the method. The method is simple and practicable, low in cost, stable in process and easy to promote. The EGCG and ECG monomers produced by the method can be used for preparing health foods and cosmetics, or used as standards.

Disclosed herein are green tea plant material extracts, and methods of using the extracts, comprising at least one green tea catechin selected from the group consisting of epi-gallocatechin, catechin, epicatechin, epigallocatechin-3-gallate, gallocatechin gallate, epicatechin-3-gallate, catechin gallate, and gallocatechin, wherein the compositions of the disclosure provide greater bioavailability of at least one green tea catechin.

Composition for promoting control of total and LDL cholesterol and / or weight loss and / or thermogenesis includes: - extract of green tea: 200-300 mg, - extract of guarana: 160-260 mg, - extract of yerba mate: 100-300 mg, - conjugated linoleic acid (CLA): 700-3400 mg, wherein the three extracts have variable concentrations of caffeine, that of green tea including at least 50% epigallocatechin gallate (EGCG), and the CLA including at least 50% trans-10, cis-12 CLA (10-12 CLA) and 50% cis-9,trans-11 CLA (9-11 CLA).

The invention discloses a method for separating and purifying an epicatechin gallate (ECG) monomer, which comprises the following steps of: with Yunnan broad-leaf green tea as a raw material, directly extracting by using ethyl acetate; chromatographically adsorbing and eluting a crude extract by two different adsorbent resin columns respectively; collecting desorption fractions containing ECG; concentrating and recovering ethanol in a high-efficiency external-circulation way and concentrating by a reverse osmosis membrane at low temperature; refrigerating and crystallizing 95% ethanol at low temperature; at last, freezing and drying to obtain 98% ECG monomer. The method disclosed by the invention is simple and convenient, short in preparation cycle and low in cost, complies with environment friendliness and safety requirements and is suitable for large-scale industrial production.

The invention provides a preparation method of high-purity epicatechin gallate (ECG), belonging to the technical field and domain of organic chemistry. The ECG content is 98.0-99.9%. The preparation method comprises the procedures of abstraction, extraction, chromatographic separation and crystallization. According to the invention, the preparation process has favorable reproducibility; and the obtained epicatechin gallate has the advantages of high content, high yield, no organic solvent residue, low cost, stable and controllable quality and short production cycle, is green, environment-friendly and suitable for industrial production, and conforms to the requirements for clean production.

The invention relates to an application of natural compound epicatechin gallate obtained from tea and derivatives thereof for treating or preventing myocardial infarction and other ischemic heart diseases. The compound is used for preventing the myocardial injury of the heart caused by ischemia-reperfusion, and is a medicament for potentially treating or preventing the heart ischemia / reperfusion injury. Particularly, the compound has a novel effect on protecting the heart and has a function that the enhancement of the myocardial contractility does not depend on the increase of the concentration of calcium ions in myocardial cells. In the invention, it is discovered that the epicatechin gallate (ECG) and the derivatives thereof have pharmacological pre-adaptation functions and have pharmacological post-adaptation functions for the first time, thus the ECG and the derivatives thereof are possibly novel medicaments for treating or preventing myocardial infarction and other ischemic heart diseases. The ECG and the derivatives thereof not only can be used for clinically treating congestive heart diseases through pharmacological post-adaptation but also can be used for medically preventing the patient who suffers from myocardial infarction and then is cured from being subjected to myocardial infarction again through pharmacological pre-adaptation.

A disclosed nontoxic natural preservative agent comprises an antioxidant extracted from tea leaves, natural vitamin E, a pectin decomposer and phytic acid (PA), and is characterized in that: the antioxidant extracted from tea leaves is a mixture of epigallocatechin, epigallocatechin-3-gallate, epicatechin gallate and catechin with a mass ratio of 1:3:2:3. The nontoxic natural preservative agent is no harmful to human body and natural and healthy.

The invention relates to a processing method of eagle tea oolong tea. The processing method comprises 6 technique links of (1) picking fresh leaves; (2) performing withering; (3) performing rolling; (4) performing fermenting; (5) performing drying; and (6) performing sieving and packing. According to the physical and chemical index detection and organoleptic review results of products processed according to different technologies of plant actinodaphne cupularis of eagle tea raw materials, and according to unique physical and chemical quality and organoleptic quality evaluation standards of excellent high-quality black tea, a black tea technology suitable for scaleable processing of actinodaphne cupularis is optimized and established. The technical parameters of picking fresh leaves, withering, rolling, fermenting, drying and the like are reported for the first time. The product pollutants, residues and the likes of products are lower than relevant limit values of hygienic indexes of NY5196-2002 organic tea, and the eagle tea oolong tea has the quality characteristics of being high in contents of caffeine and epicatechin gallate, having black, moistening and golden hairs, being orange and bright in soup color, being rich in fruity flavor, having cinnamomum longepaniculatum fragrance, and being rich and mellow in sweet after taste.

The invention described herein encompasses a methods and compositions of treating cancer or solid tumors comprising the administration of a therapeutically effective amount of catechins, a group of polyphenols found in green tea, to a mammal in need of such therapy. Compositions of catechins include but not limited to, epigallocatechin gallate (EGCg), epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC). The unique compositions of the invention contain various combinations of the catechins, alone or in combination with each other or other therapeutic agents and are used to treat primary and metastatic cancers in humans. The invention also encompasses the varying modes of administration of the therapeutic compounds.

The invention relates to a method for increasing yield of epigallocatechin-3-gallate (EGCG) and epigallocatechin gallate (ECG) by liquid-submerged fermentation of white-rot fungi by adding cellulose, hemi-cellulose and lignin, fatty acids, organic solvents and surfactants. Fermentation in a liquid fermentation tank is carried out with preparation of catechins by fermentation white-rot fungi such as inonotus obliquus as an object and Holland CBS culture collection inonotus obliquus as initial strains. Fermentation conditions are as follows: a temperature is 27-28 DEG C; an air flow is 0.5-1.5 vvm; a stirring speed is 80-160 rounds per minute; an internal pressure is 0.15-0.35 kilograms per cubic centimeter; and a fermentation cycle is 10-14 days. The method can increase the yield of epigallocatechin-3-gallate (EGCG) and epigallocatechin gallate (ECG) by liquid-submerged fermentation of the inonotus obliquus.

Compositions and methods of increasing the bioavailability of catechins are presented. Compositions for increasing the bioavailability of catechins include compositions where the catechin is added to a solution of ethanol and water; compositions where the catechin is encapsulated within a nanoparticle; and compositions in which a nanoparticle complex is formed between the catechin and the nanoparticle. Each of these compositions was shown to increase bioavailability of EGCG and is useful in treating diseases such as Alzheimer's and HIV-associated dementia.

The invention relates to a preparation method of an epicatechin monomer. The preparation method comprises the following steps of: (1) adding tannase to a solution containing large-leaf green tea catechin extract, carrying out static enzymolysis at 30-35 DEG C, intermittently stirring, and centrifugally filtering to obtain an enzymolysis solution; (2) introducing the enzymolysis solution into a chromatographic column loaded with adsorptive resin, carrying out gradient elution with ethanol elution agent, collecting eluate, concentrating eluate to 16-18 Baume degree to obtain concentrate, standing the concentrate at 0-4 DEG C for 48-72h, adding a little of epicatechin monomer, crystallizing and recrystallizing, centrifuging and drying to obtain the epicatechinmonomer. According to the method, epicatechin gallate is subjected to enzymolysis by using tannase, so that the method has good transformation effect, high transformation rate and high product quality, short preparation period, and low cost, and is suitable for scaled production.

The invention discloses an environmentally-friendly anti-microbial inactivator for treating human papilloma virus infection. The environmentally-friendly anti-microbial inactivator is prepared from the components in percentage by mass: 0.1 to 0.2 percent of cation antimicrobial, 1.2 to 2.0 percent of epicatechin gallate, 1.2 to 2.0 percent of honeysuckle flower, 2.0 to 4.0 percent of radix paeoniae rubra, 2.0 to 5.0 percent of ethyl alcohol, 0.6 to 1.0 percent of herba menthae, and the balance of purified water to complement to 100 percent. According to the environmentally-friendly anti-microbial inactivator provided by the invention, an antibacterial effect is good after dodecyl dimethyl benzyl ammonium chloride, didecyl dimethyl ammonium chloride and ammonium dimethylalkyl oxide are mixed, the antibacterial rate of a cation antimicrobial on poliomyelitis virus sterilization log value is larger than 4 within 1 minute.

Disclosed is a method for protecting living body in radialization of UV-B using catechuic acid, wherein catechuic acid is employed to preventing living body from radialization damage of UV-B, or recovering the living body damaged by UV-B. The content of [(-)-epigallocatechin-3-gallate(EGCG)] in the catechin is not lower than 40%, the content of ECG is not lower than 15%, the content of caffeine is no less than 2.5%.