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Preparation method for grape seed proanthocyanidin extract

A technology of proanthocyanidins and grape seeds is applied in the field of preparation of grape seed proanthocyanidins, and can solve the problems of low extraction efficiency, low product yield and purity, etc.

Inactive Publication Date: 2012-09-26
哈尔滨市海澳斯生物科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the extraction methods of grape seed anthocyanins at home and abroad mainly include solvent extraction, microwave method and supercritical CO 2 Extraction method, etc., the yield and purity of the products obtained by these methods are low, the main reason is that proanthocyanidins form a dense combination with high molecular weight cellulose and hemicellulose in grape seeds, which is difficult to open in a solvent or microwave state, which directly leads to Extraction efficiency is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1. Selection of fermentation strains and preparation of medium

[0043] The fermentation strains were purchased from the China Industrial Microbiology Culture Collection Management Center, which are as follows:

[0044] Bacteria A - Trichoderma konningen, Trichoderma koningii The number is: CICC40505, the culture medium is: take 200 grams of peeled potatoes, cut into small pieces, add 1000 ml of water and boil for 30 minutes, filter out the potato pieces, make up the filtrate to 1000 ml, add 20 grams of glucose, dissolve and pack , 15 pounds of sterilization for 30 minutes.

[0045] B bacteria - Aspergillus niger, Aspergillus niger , No.: CICC41130, culture medium: 30 grams of sucrose, 2 grams of sodium nitrate, 0.5 grams of magnesium sulfate, 0.5 grams of potassium chloride, 0.01 grams of ferrous sulfate, 1 gram of dipotassium hydrogen phosphate, 1000 milliliters of distilled water, 15 pounds of Bacteria for 30 minutes.

[0046] 2. The preparation process of the d...

Embodiment 2

[0075] 1. Selection of fermentation strains and preparation of medium

[0076] The fermentation strains were purchased from the China Industrial Microbiology Culture Collection Management Center, which are as follows:

[0077] Bacteria A - Trichoderma konningen, Trichoderma koningii The number is: CICC40505, the culture medium is: take 200 grams of peeled potatoes, cut into small pieces, add 1000 ml of water and boil for 30 minutes, filter out the potato pieces, make up the filtrate to 1000 ml, add 20 grams of glucose, dissolve and pack , 15 pounds of sterilization for 30 minutes.

[0078] B bacteria - Aspergillus niger, Aspergillus niger , No.: CICC41130, culture medium: 30 grams of sucrose, 2 grams of sodium nitrate, 0.5 grams of magnesium sulfate, 0.5 grams of potassium chloride, 0.01 grams of ferrous sulfate, 1 gram of dipotassium hydrogen phosphate, 1000 milliliters of distilled water, 15 pounds of Bacteria for 30 minutes.

[0079] 2. The preparation process of the d...

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Abstract

The invention provides a preparation method for a grape seed proanthocyanidin extract. The preparation method comprises the following steps: firstly, preparing trichoderma koningii and aspergillus niger direct vat set; and secondly, adding water into grape seeds for grinding; adding glucose for mixing, sterilizing at high temperature, inoculating the bacteria and fermenting, centrifugally removing slag, carrying out ultrafiltration extraction, drying and other processes to obtain the grape seed proanthocyanidin extract.

Description

[0001] (1) Technical field [0002] The invention relates to a preparation method of grape seed proanthocyanidins, in particular to a method for extracting proanthocyanidins after fermenting grape seed pulp with two strains of fungi, which belongs to the field of bioengineering. (2) Background technology [0003] In recent years, the biological activity of polyphenols and their health care and disease prevention effects on the human body have attracted increasing attention. Free radicals are considered to be one of the causes of human aging and certain chronic diseases. When the free radicals in the human body are produced too much or cleared too slowly, they will turn to attack various cells and organs and cause them to be damaged, accelerate the aging process of the body and induce various diseases. The molecular structure of polyphenolic compounds contains several phenolic hydroxyl groups, which have a strong ability to scavenge free radicals, thereby protecting biologic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P17/16C12R1/885C12R1/685
Inventor 佟晓芳陈成姜帆
Owner 哈尔滨市海澳斯生物科技开发有限公司
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