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Extraction method of procyanidine in grape seeds

A proanthocyanidin and extraction method technology, which is applied in the field of grape seed proanthocyanidin extraction, can solve the problems of low membrane flux, high cost investment, and low processing capacity, and achieve the effects of strong antioxidant activity, low cost input, and increased content

Active Publication Date: 2018-05-08
北京颐方生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the extraction method of proanthocyanidins involved in the above-mentioned documents, enzymes are used to degrade cellulose to facilitate the dissolution of proanthocyanidins, the cost input is relatively high, and mild conditions suitable for enzymatic hydrolysis are required in the enzymatic hydrolysis process, and the operation is relatively complicated
In the process of extracting proanthocyanidins with specific molecular segments by ultrafiltration membrane filtration, there are still defects such as low membrane flux, large membrane area required, low processing capacity, long ultrafiltration time, and difficulty in achieving large-scale industrial production.

Method used

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  • Extraction method of procyanidine in grape seeds

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Effect test

Embodiment 1

[0040] The present embodiment provides a kind of extraction method of grape seed proanthocyanidins, comprises the following steps:

[0041] 1) Grape seed pretreatment

[0042] Select non-mildew grape seeds, put them into the cleaning pool, wash them with sterile water for 3 times, drain or use dried grape seeds as raw materials, soak the dried grape seeds in sterile water for 2 hours to make them fully absorb water, Soak and rinse and drain. Put the drained grape seeds into the roller compactor, so that the grape seeds are crushed through a 1.0mm compaction gap. After being processed by the compactor, the grape seeds become flat, and the seed coat is crushed into several cracks. Endosperm exudation is clearly visible.

[0043] 2) Preparation of fermented grape seeds

[0044] a) Strain activation: Inoculate Aspergillus niger strains on a test tube slant or flat plate containing potato medium through sterility verification, and then cultivate the inoculated slant or flat plat...

Embodiment 2

[0058] The present embodiment provides a kind of extraction method of grape seed proanthocyanidins, comprises the following steps:

[0059] Wherein step 1)-step 2) is identical with embodiment 1.

[0060] 3) Preparation of proanthocyanidin extract

[0061] Three-level four-tank (tank body numbers are A, B, C, D) type countercurrent extraction method is adopted. The specific operation is to add 60 kg of fermented grape seeds to each tank, and add 85% ethanol solution with a pH of 4.5 (adjusting the pH with acetic acid) 200L was added to tank A, stirred and leached for 20 minutes at a temperature of 75°C to obtain extract A1, and store A1. Add the same amount of solvent into tank A, stir and extract under the same conditions for 15 minutes to obtain extract A2; then pump the extract A2 into tank B, and extract under the same extraction conditions to obtain Extract B1, store B1. Add the same amount of solvent into tank A, and extract under the same conditions. After the extracti...

Embodiment 3

[0065] The present embodiment provides a kind of extraction method of grape seed proanthocyanidins, comprises the following steps:

[0066] Wherein step 1)-step 4) is identical with embodiment 1.

[0067] 5) Refined proanthocyanidins

[0068] Soak the AB-8 macroporous resin in ethanol for 24 hours, rinse with ethanol until the effluent is mixed with distilled water without turbidity, and then rinse with distilled water until the effluent has no ethanol smell. Pack the pretreated adsorption resin into a column (the ratio of diameter to height of the chromatography column is 1:30), and the resin is required to be evenly dispersed and free of air bubbles. The crude product of proanthocyanidin was weighed and prepared into an aqueous solution with a concentration of 1.5%, filtered to remove insoluble impurities, and the proanthocyanidin solution was passed through a column. Desorb with 4 times the column volume of 25% (volume fraction) ethanol solution, collect the eluate and di...

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Abstract

The invention relates to an extraction method of procyanidine in grape seeds and overcomes the problems that procyanidine in grape seed husk is difficultly dissolved out and the polymerization degreeof the procyanidine in the extract is high are overcome. According to the extraction method of the procyanidine in the grape seeds, aspergillus niger is used as strain for fermenting the grape seeds;the enzymes such as cellulase and pectinase produced by the aspergillus niger are used for degrading the grape seed husk, so that the procyanidine is more easily dissolved out; alcohols with differentvolume fractions are used as desorption solutions for eluting macroporous adsorption resin; the procyanidine is graded and is subsequently subjected to partially acidolysis, so that the content of procyanidine oligomer in the extract is increased. The content of the oligomerized procyanidine in the extract is greater than or equal to 98%; the average polymerization degree of the procyanidine is 2.5-3.0; the procyanidine is easily absorbed in the body and is high in anti-oxidation activity.

Description

technical field [0001] The invention belongs to the field of natural product active substance extraction, and in particular relates to a method for extracting grape seed proanthocyanidins. Background technique [0002] Proanthocyanidins (proanthocyanidins), also known as condensed tannins, are molecules with a certain degree of polymerization connected by flavan-3 alcohol monomers through C4-C8 or C4-C6 positions, and exist in the form of free, oligomeric and polymeric Its basic units are mainly catechin, epicatechin, gallic acid and epicatechin gallate. According to the degree of polymerization, it can be divided into oligomeric proanthocyanidins (referred to as OPC, two, three, and tetramers of the basic unit) and high-polymerized proanthocyanidins (abbreviated as PPC, the degree of polymerization of the basic unit is greater than 4). Among them, oligomeric proanthocyanidins can be absorbed and utilized by the human body, and have higher antioxidant activity than their co...

Claims

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Application Information

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IPC IPC(8): C07D311/62A61P3/10A61P39/06A23L33/105
CPCA23L33/105A23V2002/00C07D311/62A23V2200/30A23V2200/328A23V2250/21166
Inventor 石清东王正元陈赫岩王姣
Owner 北京颐方生物科技有限公司
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