40 results about "Glutathione Transferases" patented technology

Peptides having general and specific binding affinities for the Src homology region 3 (SH3) domains of proteins are disclosed in the present invention. In particular, SH3 binding peptides have been isolated from three phage-displayed random peptide libraries which had been screened for isolates that bind to bacterial fusion proteins comprising SH3 and glutathione S-transferase (GST). Preferred peptides are disclosed which comprise a core 7-mer sequence (preferably, a consensus motif) and two or more, preferably at least six, additional amino acid residues flanking the core sequence, for a total length of 9, preferably at least 13, amino acid residues and no more than about 45 amino acid residues. Such peptides manifest preferential binding affinities for certain SH3 domains. The preferred peptides exhibit specific binding affinities for the Src-family of proteins. In vitro and in vivo results are presented which demonstrate the biochemical activity of such peptides.

Polyacrylamide-based methods of fabricating surface-bound peptide and protein arrays, the arrays themselves, and a method of using the arrays to detect biomolecules and to measure their concentration, binding affinity, and kinetics are described. Peptides, proteins, fusion proteins, protein complexes, nucleic acids, and the like, are labeled with an acrylic moiety and attached to acrylic-functionalized glass surfaces through a copolymerization with acrylic monomer. The specific attachment of glutathione S-transferase-green fluorescent protein (GST-GFP) fusion protein was more than 7-fold greater than the nonspecific attachment of non-acrylic labeled GST-GFP. Surface-attached GST-GFP (0.32 ng / mm2) was detectable by direct measurement of green fluorescent protein fluorescence and this lower detection limit was reduced to 0.080 ng / mm2 using indirect antibody-based detection. The polyacrylamide-based surface attachment strategy was also used to measure the kinetics of substrate phosphorylation by the kinase c-Src which is encoded by the Rous Sarcoma virus. The surface attachment strategy is applicable to the proteomics field and addresses denaturation and dehydration problems associated with protein microarray development.

The invention relates to a gene expression product BLSJ-1 with Brucella diagnosis and identification effect, and a preparation method thereof. According to the preparation method, the gene expression product is obtained via gene prokaryotic expression of Brucella S2 strain with GI of 489054867, and via glutathione S transferase (GST) affinity chromatographic column and glutathione gradient elution purifying. The gene expression product can be taken as a coating antigen, and as a diagnosis antigen of animal Brucella vaccine immunity and naturally infected serum detection. When the BLSJ-1 antigen is subjected to western blot with vaccine immune antibodies and natural infection antibodies, the difference is obvious. The gene expression product BLSJ-1 is taken as an indirect enzyme-linked immune adsorption assay (ELISA) coating antigen to detect hundreds of brucellosis clinical serum samples, and differential diagnosis effect is excellent.

The invention discloses a method for high-throughput screening of single-domain antibodies using isPLA. The steps are as follows: constructing an sdAb library containing 21 random amino acid sequences in the CDR3 region, and fusing a 3×Flag tag to the C-terminus of the sequence; co-transfection Cells, fixed cells for isPLA; followed by sorting, amplification, and recombination, and after multiple rounds of screening and sequencing, constructs that sequentially contain glutathione S-transferase GST, tobacco mosaic virus TEV protease cleavage site, and recognize SQSTM1. The sdAb sequence, the translocation domain of Pseudomonas exotoxin ETA and the 3×Flag-tagged recombinant protein were expressed in E. coli, followed by purification and enzyme digestion. The method can screen sdAbs that specifically recognize different antigenic determinants from the constructed high-capacity sdAb library, and has low cost and high efficiency. No preparation of animal samples or hybridomas is involved.

The present invention relates to a novel method for detoxification of trichothecene contaminated materials. More specifically, the present invention relates to a method of bioconverting trichothecene by contacting a material contaminated with trichothecene with an exogenous non-animal glutathione-S-transferase (GST) having substrate specificity for the epoxy ring of trichothecene. The invention also relates to recombinant GST as well as to transgenic plants and animals expressing said GST.

The invention relates to a gene expression product BLSJ-3 with Brucella diagnosis and identification effect, and a preparation method thereof. According to the preparation method, the gene expression product is obtained via gene prokaryotic expression of Brucella S2 strain with GI of 490819668, and via glutathione S transferase (GST) affinity chromatographic column and glutathione gradient elution purifying. The gene expression product can be taken as a coating antigen, and as a diagnosis antigen of animal Brucella vaccine immunity and naturally infected serum detection. When the BLSJ-3 antigen is subjected to western blot with vaccine immune antibodies and natural infection antibodies, the difference is obvious. The gene expression product BLSJ-3 is taken as an indirect enzyme-linked immune adsorption assay (ELISA) coating antigen to detect hundreds of brucellosis clinical serum samples, and differential diagnosis effect is excellent.