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Biomarker for evaluating zinc nutrition state of individual and application thereof

A zinc nutrition and individual technology, applied in the field of biomarkers, can solve the problems of lack of sensitive and specific indicators

Pending Publication Date: 2021-04-20
HARBIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although there are many zinc deficiency evaluation indicators, there is still a lack of sensitive and specific indicators

Method used

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  • Biomarker for evaluating zinc nutrition state of individual and application thereof
  • Biomarker for evaluating zinc nutrition state of individual and application thereof
  • Biomarker for evaluating zinc nutrition state of individual and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Zinc deficiency animal model establishment and multi-omics experiment screening candidate biomarkers

[0042] 1. Feeding and sample collection of zinc-deficient rats:

[0043] 48 male Wistar rats with a body weight of 60-80g at the age of 3 weeks were randomly divided into 3 groups after being adaptively fed for 7 days in a separate metabolic cage: control group (15 rats): normal zinc diet (zinc content is 30mg / ml) Pure synthetic feed); Zinc deficiency group (15): low zinc diet (pure synthetic feed with zinc content of 10mg / ml); pair feeding group (15): normal zinc diet (pure synthetic feed with zinc content of 30mg / ml) feed), the feed intake was the same as that of the low zinc group. All rats were continuously fed for 4-6 weeks. Dynamic monitoring of basic indicators such as rat body weight, food intake, and urine output, and energy metabolism detection of energy and meal consumption.

[0044] 2. Relevant index detection:

[0045] After the experiment wa...

Embodiment 2

[0094] Example 2 Validation of Zinc Deficiency Population of Zinc Deficiency Biomarkers

[0095] Through two zinc deficiency susceptible populations: children aged 4-6 and low-income manual laborers (age 45-58), grouped by serum zinc, the candidate markers screened in animal experiments were verified to determine the biomarkers of zinc deficiency in the population. A randomized controlled zinc supplementation intervention trial in zinc deficient populations to further identify biomarkers of zinc deficiency in the population. Expand the sample size, conduct whole-population experiments, quantitatively detect the concentration of biomarkers, and determine the normal range of biomarkers.

[0096] The ultimate goal expected to be achieved is to establish a standard method for the nutritional assessment of zinc deficiency.

[0097] The study recruited children (4-6 years old) as the children group and low-income manual labor middle-aged people (40-58 years old) as the middle-aged ...

Embodiment 3

[0117] Example 3 Zinc Supplementation Intervention Experiment for Zinc Deficient Crowds

[0118] In the middle-aged group, people with low zinc levels were selected for zinc supplementation intervention experiment. This study was a randomized double-blind controlled trial. The experiment lasted for 2 weeks. The researchers drew fasting venous blood from the participants in the first week and the second week respectively. Serum zinc and biomarker concentrations were detected to verify the sensitivity and specificity of the biomarkers. Throughout the experiment, dietary questionnaires were administered to the population to calculate dietary zinc intake. At the same time, other interfering factors were excluded, and the correlation analysis was used to evaluate the changes of biomarkers, serum zinc levels and zinc intake levels, as well as the diagnostic ability of ROC curve analysis biomarkers.

[0119]Experimental process: Zinc supplementation intervention experiment recruited...

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PUM

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Abstract

The invention discloses a biomarker for evaluating the zinc nutrition state of an individual and application of the biomarker. Wherein the biomarker is glutathione S-transferase Omega1 (GSTO1). Differential biomarkers (proteins and metabolites) in blood and liver of rats lack of zinc are screened through a multi-omics combined technology based on high-flux chromatography-mass spectrometry, such as proteomics and metabonomics; enrichment analysis is carried out on the multi-omics metabolic pathways to screen the most significant metabolic pathways with zinc deficiency and candidate biomarkers with zinc deficiency are screened; the screened candidate zinc deficiency biomarkers are verified through zinc deficiency sensitive population; and finally, the specific zinc deficiency biomarker of the crowd is determined through a random contrast zinc supplement intervention experiment of the zinc deficiency crowd, a rapid, sensitive and specific standard method system capable of being used for zinc nutrient state evaluation is tried to be established based on the screened biomarker, and help is provided for evaluating the zinc nutrient state and intervention measures of individuals.

Description

technical field [0001] The invention relates to a biomarker that can be used for the diagnosis of zinc deficiency in crowds and its application. The invention belongs to the technical field of medicine. Background technique [0002] Zinc is an essential micronutrient for the human body. It has extremely extensive and important physiological functions, and plays important structural and functional roles in a variety of enzyme systems involved in gene expression, cell division and cell growth, as well as immune and reproductive functions. Zinc deficiency seriously endangers human health, such as affecting children's growth and development, sexual function decline, skin diseases, cognitive disorders, anorexia, increasing the risk of infection, and affecting pregnancy outcomes, etc. [0003] According to the data of food utilization, WHO estimates that the prevalence of dietary zinc deficiency in the world is about 31% (4%-70%). my country's health and nutrition survey shows t...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86G01N33/53
Inventor 王茂清孙长颢李颖
Owner HARBIN MEDICAL UNIVERSITY
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