The invention belongs to the field of medicine, and particularly provides a kit for detecting cervical carcinoma and a detection method. The genes of SOX1, PAX1, LMX1A, NKX6-1, WT1 and ONECUT1 are tumor suppressor genes related to the cervical carcinoma, and the methylation of CpG islands of promoter sites of the genes is one of main reasons for the inactivation of the genes. In the kit and the detection method, a patient can be clearly determined whether to suffer from the cervical carcinoma or not by detecting the methylation of the CpG islands of the promoter sites of the genes of SOX1, PAX1, LMX1A, NKX6-1, WT1 and ONECUT1 respectively and detecting whether HPV-L1 exists or not, the methylation of the genes is treated in advance and outcome is detected by using the HPV-L1 proteins, so that the actual conditions of cervical canceration and outcome of the patient can be perfectly evaluated in terms of molecular level, treatment is effectively guided, and misdiagnosis, over-treatment and missed diagnosis in the conventional treatment of the cervical carcinoma can be further avoided.