CpG insular methylation test reagent kit and its application

Abstract

The present invention relates to gene determining technology and is especially CpG insular methlation test reagent kit and its application for diagnosing tumor and screening demethylation medicine. The reagent kit includes primer, medicine for extracting template DNA, medicine for modifying genome, medicine for purifying DNA, specific MSP-PCR reaction system and negative and positive contrast samples. The present invention utilizes human gene sequences and designs primer based on base mismatch principle, and different gene primer is selected to constitute different reagent kit for detecting different diseases. The present invention may be used widely in the diagnosis of cancer and the screening of relevant medicine.

Description

technical field [0001] The invention relates to gene detection technology, in particular to a CpG island methylation detection kit for tumor diagnosis and demethylation drug screening and its application. Background technique [0002] A CpG island is a region of DNA with a length of about 0.5-2.6kb. CpG islands are unmethylated in normal tissues. But in human malignancies, altered DNA methylation is one of the most commonly recognized genetic abnormalities. Hypermethylation of many specific gene manipulation regions generally occurs at an early stage in malignant tumors. [0003] The current method for measuring the methylation of 5′-CpG islands is Southern blot. The principle is that the methylation-sensitive restriction endonuclease cannot cut the sequence containing one or several methylated CpG sites. The method provides an estimate of the overall methylation of CpG islands, requiring a large number of DNA components, which can provide information on the methylation o...

AI Technical Summary

Problems solved by technology

However, there are currently no reports on the production, sales and use of kits for detecting tumor gene methylation and demethylation at home and abroad.