Detection method for food quality
A detection method and technology for food quality, applied in the field of detection, can solve problems such as complex chemical components, and achieve the effect of high sensitivity
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Embodiment 1
[0032] Take four portions of 10g green vegetables sprayed with different concentrations of pesticides, cut them into shreds, and mark them as D1, D2, D3, and D4. Put D1, D2, D3, and D4 green vegetables in an organic solvent of turpentine derivatives. Vegetables and organic The solvent is mixed according to the solid-liquid ratio of 0.2g / ml, and the ultrasonic extraction time is 4min, which is carried out twice. After the ultrasonic extraction is completed, activated carbon and anhydrous sodium sulfate are added for centrifugal purification. After microfiltration of vinyl fluoride microfiltration membrane and nanofiltration of NF90-4040 nanofiltration membrane, blow off the extractant in the eluent; add a chelating agent to the four extracts obtained above after blowing off the solvent for mixing, and then add Reduced glutathione and glutathione transferase catalyze a mixed reaction, and the content of reduced glutathione in the mixture is 5×10 -5 mol / L, glutathione transferase...
Embodiment 2
[0035] Take four 10g loofahs sprayed with different concentrations of pesticides, cut them into pieces, and label them as H1, H2, H3, and H4. Put the loofahs in groups H1, H2, H3, and H4 in organic solvents of turpentine derivatives. Vegetables and organic solvents The mixture was mixed according to the solid-liquid ratio of 0.5g / ml, and the ultrasonic extraction time was 4min, which was carried out twice. After the ultrasonic extraction was completed, activated carbon and anhydrous sodium sulfate were added for centrifugal purification. After ethylene microfiltration membrane microfiltration and NF90-4040 nanofiltration membrane nanofiltration, blow off the extractant in the eluent; add a chelating agent to the four extracts obtained above after blowing off the solvent for mixing, and then add Prototype glutathione and glutathione transferase catalyze a mixed reaction, and the content of reduced glutathione in the mixture is 1×10 -5mol / L, glutathione transferase content is 0....
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