The invention discloses hair lotion for preventing hair loss and promoting hair growth, containing ganoderma niger lloyd plant extract essence and a preparation method of the hair lotion. The hair lotion comprises the following raw materials of water, a ganoderma niger lloyd extract, a ginseng root extract, a ligusticum wallichii extract, a fleeceflower root extract, a radix angelica sinensis extract, a Chinese soapberry seed peel extract, amine laureth sulfate, cocamidopropyl betaine, a Chinese honeylocust fruit extract, ammonium lauryl sulfate, polyquaternary ammonium salt-47, a camellia seed extract, cocamide methyl MEA, camellia seed oil, glycerine, a black mulberry extract, panthenol, sodium C10-16 pareth sulfate, a Chinese arborvitae twig and leaf extract, cetearyl alcohol, Guar hydroxypropyltrimonium chloride, a ginger root extract, PPG-3 myristyl ether, biotin, citric acid, a radix sophorae flavescentis extract, phenoxyethanol, essence, and an astragalus complanatus extract. The product is used, so that hair follicle can be activated, hair growth can be promoted, the hair can be nourished and cared, hair quality can be effectively improved, the pH of the hair can be effectively adjusted, and the hair lotion has the effects of diminishing inflammation and restraining bacteria, and can enable the head to be refreshing and clean.