The invention relates to a honeycomb low-temperature SCR denitration catalyst and a preparation method thereof, and belongs to the technical field of flue gas denitration. The preparation method comprises the following steps: A, mixing: 100 parts by weight of titanium-molybdenum powder, 8-20 parts by weight of carboxymethyl cellulose, 5-10 parts by weight of polyethylene oxide, 5-7.5 parts by weight of silicon dioxide powder, 5-7.5 parts by weight of silica sol, 7-18 parts by weight of wood pulp, 25-50 parts by weight of glass fiber, 50-80 parts by weight of deionized water, 5-15 parts by weight of an ammonium metavanadate solution, 10-20 parts by weight of an ammonium metatungstate solution, 30-55 parts by weight of ammonia water and 10-20 parts by weight of lactic acid are added into a mixer to be fully mixed; water vapor in the mixer is pumped after the mixing, and the molybdenum content of the titanium-molybdenum powder is greater than or equal to 7%; B, molding; C, drying; and D,calcination. By the preparation method, the energy consumption is reduced, and the denitration efficiency of the obtained honeycomb low-temperature SCR denitration catalyst at the temperature of 160 to 250 DEG C reaches 83% and above.