54results about How to "Short preprocessing time" patented technology

The invention provides a method for detecting and pretreating polycyclic aromatic hydrocarbon in oily sludge. The method comprises the steps of taking the oily sludge, feeding kieselguhr into the oily sludge, grinding, sieving, adding a substitute, evenly mixing and then extracting with extract liquor; collecting the extract liquor, and concentrating to obtain extract concentrated solution; filling a chromatographic column; after the chromatographic column is balanced, feeding the extract concentrated solution sample onto the chromatographic column, eluting the chromatographic column with n-hexane after the chromatographic column comes into full contact with the extract concentrated solution; eluting the chromatographic column with a mixed eluant of dichloromethane and n-hexane; collecting the eluant until the mixed eluant of dichloromethane and n-hexane completely flows outside, thus obtaining the mixed liquid of the polycyclic aromatic hydrocarbon; concentrating the mixed liquid of the polycyclic aromatic hydrocarbon, and carrying out nitrogen purging concentration to obtain the polycyclic aromatic hydrocarbon sample. With adoption of the rapid extraction method, the use amount of an organic solvent is small, the pretreatment time is short and operation is simple.

The invention discloses a pretreatment method for a liquid sample in a laser-induced breakdown spectrum detection technology. The pretreatment method comprises the following steps: (1), fetching a liquid sample and removing insoluble impurities; (2), adding a precipitator and uniformly mixing; (3), filtering the liquid sample treated by the step (2) by a microfiltration membrane with an aperture not larger than 0.2 micrometer, and removing excessive liquid on the filtration membrane to obtain a filtration membrane. According to the invention, precipitation enrichment is carried out on heavy metal in the liquid sample by the precipitator, the enrichment capability of the heavy metal is greatly improved, the sampling quantity of the liquid sample is reduced, detection can be carried out by only 1ml of sampling quantity, the detection sensitivity and accuracy are high, the detection limit can reach 10-1 to 10 ppb, and various metal elements can be simultaneously detected. Meanwhile, the pretreatment method also has the advantages of short pretreatment time, simple step, low cost and the like and has an excellent application prospect.

The invention relates to a different-strip-aerial-image-based power sag detection method and system. The system is composed of a calibration selection module, a main control module, a space three-dimensional image pair module, a three-dimensional space model measurement module, and a diagnosis module. The calibration selection module calibrates a flight path and selects a field control point; the main control module controls an unmanned aerial plane to fly according to the flight path and controls the unmanned aerial plane to collect space position information of a photo; the space three-dimensional image pair module establishes a space three-dimensional image pair of a power sag according to the field control point and the space position information of the photo; the three-dimensional space model measurement module identifies a dotted point of the power sag in the space three-dimensional image pair and establishes a three-dimensional space model of the power sag; and the diagnosis module determines a dangerous point on the power sag according to the three-dimensional space model and inputs a detection report automatically. Continuous aerial photos are collected by using the unmanned aerial plane, the identified three-dimensional image queue sequence is constructed in the different strip photos, and space intersection measurement is carried out, thereby completing precise sag measurement of the whole line.

A method for pre-treating an epitaxial layer performed before evaluation of the epitaxial layer by making the epitaxial layer contact with a metal electrode by a capacitance-voltage measurement, the method comprising; applying carbon-bearing compound to a surface of the epitaxial layer; subsequently irradiating ultraviolet light to the surface of the epitaxial layer; and thereby forming an oxide film on the surface of the epitaxial layer.

The invention discloses a pre-treatment method used for increasing residual activated sludge anaerobic fermentation hydrogen production efficiency. The pre-treatment method comprises following specific steps: municipal sewage plant secondary sedimentation tank sludge is taken as a fermentation base material, ammonium chloride is added into the fermentation base material, fermentation system pH value is adjusted, at a constant temperature, a certain free ammonia (FA) concentration is achieved for 12h of pre-treatment, and anaerobic fermentation is carried out for hydrogen production. The free ammonia based pre-treatment is capable of promoting dissolving out of organic matters in sludge, fully inhibiting the activity of hydrogen-consuming microorganisms such as acetogenic bacteria and methanogens of the same type, and increasing hydrogen production efficiency greatly. The free ammonia pretreatment hydrogen production rate is as high as 14.65ml H2 / g VSS, and is 14.22 times of that of a blank group, and is 2.41 times of that of an alkalescence pretreatment group (pH is 9.0+ / -0.1). The pre-treatment method is simple in operation and high in hydrogen production efficiency; adding of additional special bacterial strains as hydrogenogens is not necessary; cost is low; sludge quantitative reduction, resource recycling, and stabilization can be realized at the same time; and important environmental protection ecological meaning is achieved.

The invention discloses a method for improving slurry-forming ability of sludge and coal-water slurry by cracking sludge flocculation through ultrasonic waves. The ultrasonic waves are used for modifying sludge, microbial cell walls in a sludge floc structure and the sludge are broken by high-temperature high-pressure environments and shearing action generated by ultrasonic cavitation action, sludge particle granularity is reduced, part of interstitial water is released, the modified sludge is mixed with coal, slurry-forming additives, water and the like to prepare the sludge and coal-water slurry, or finished coal-water slurry is doped into the modified sludge and uniformly stirred to prepare the sludge and coal-water slurry. Compared with the prior art, the method has the advantages that the slurry-forming viscosity of the sludge and coal-water slurry can be obviously reduced, slurry-forming concentration is increased, the sludge and coal-water slurry with excellent slurry-forming ability is obtained, treatment time is short, operation is convenient, and the method is applicable to large-scale popularization and application.

The invention discloses an indoor gorgon euryale planting method. The indoor gorgon euryale planting method comprises the steps of seed germination accelerating, planting, management after budding, harvesting and storage and package. According to the indoor gorgon euryale planting method, seeds are subjected to mechanical, hormone and low-temperature treatment, the time is short, germination is quick, and the germinating rate is up to 95.6%. The growing conditions of gorgon euryale are strictly controlled at different stages, the gorgon euryale can be planted all year around, and the shortcoming that one-time harvesting can be only performed each year in traditional planting is overcome; indoor cultivation is adopted, the environment is clean and tidy, a small amount of water is changed every day to keep water fresh and free of weeds, a traditional Chinese medicine disinfection bag is soaked in a pond to inhibit gorgon euryale diseases and insect pests, manpower and material resources are saved, pesticide residues are avoided, and edible safety is ensured. Low-temperature baking and drying are adopted, the drying speed is high, the bright color of the gorgon euryale is kept, nutritional loss is small, moisture regaining does not occur, the gorgon euryale is easy to store, the cool nature of the gorgon euryale can be removed through repeated drying to make drug properties slightly mild, a health-care effect is improved, and the gorgon euryale is beneficial to digestion and absorption and also reinforces the spleen and kidney.

The invention discloses an immunoaffinity column for detecting vomitoxin and application of the immunoaffinity column. The immunoaffinity column comprises a solid-phase extraction column hollow column (1), an upper sieve plate (2), an upper cover (3), a lower cover (4), a solid-phase medium (5) and a lower sieve plate (6), wherein the solid-phase medium is coupled with a vomitoxin antibody. The invention further provides a method for detecting vomitoxin residues in grains and feed by adopting the immunoaffinity column for detecting vomitoxin. The immunoaffinity column disclosed by the invention has the characteristics of simplicity in operation, high sensitivity, high detection speed, low cost and the like, and is suitable for screening a lot of samples and monitoring in the field.

The invention relates to a sample preparation method used for determining abundance of a <15>N isotope of nitrite. The sample preparation method comprises the following steps: loading a sample, namelyloading a <15>N-labeled to-be-prepared sample and aqueous solution of a reducing agent into a first bulb of a double bulb reaction tube, and loading an acidic material into a second bulb of the double bulb reaction tube; preparing a <15>NO gas, namely maintaining vacuum degree, and adding the acidic material in the second bulb into the first bulb, so that the <15>NO gas is generated; detecting, namely after the vacuum degree of a pipeline between a vacuum plug valve and a gas isotope ratio mass spectrometer is reduced to be below 2*10<-5>Pa, opening the vacuum plug valve, and introducing the<15>NO gas in the first bulb into the gas isotope ratio mass spectrometer; setting detection parameters of the gas isotope ratio mass spectrometer, detecting a relative intensity when mass numbers are30 and 31, and performing a parallel test for multiple times; and calculating an abundance value of the <15>N isotope. Compared with the prior art, the sample preparation method provided by the invention has the advantages that an operation process is safe and reliable, sample pretreatment time is greatly shortened, economic cost is low and test data accuracy and precision are high.

The invention discloses a zirconium group hydrophile chromatographic column and an application thereof in measuring the melamine content in a raw milk or dairy product. Melamine in a sample is extracted in an ultrasonic, oscillating and centrifuging manner by using a 1% trichloroacetic acid-acetonitrile solution, and HPLC or LC-MS / MS segregation analysis is carried out on sample liquid filtered through a 0.2um filter membrane by using the zirconium group hydrophile chromatographic column. The method for measuring the melamine content in the raw milk or dairy product by using the chromatographic column is simple and convenient, rapid, sensitive and accurate, high in repeatability and low in detection cost. The separating mode is beneficial to the preparation of samples, so that the sample pretreatment is simple in operation, rapid, and high in recovery rate, has no interference on analysis and measurement, the samples can be treated in a batch manner, the time is saved, the measuring cost is reduced, the chromatographic column has long service life, and the indexes are superior to those by using a GB / T22388-2008 method. The method can be widely applied to detecting the melamine in the raw milk or dairy products.

The invention relates to a processing method for intensifying enzymolysis saccharifying of lignocelluloses. The method comprises the following steps: respectively screening oil tea cake and lignocelluloses biomass and then drying; mixing the dried oil tea cake and lignocelluloses biomass in a mass ratio of 1:(10-20), thereby acquiring a mixture A; mixing the mixture A with deionized water, thereby acquiring a reaction solution; performing ultrasonic treatment on the reaction solution; after ending the ultrasonic treatment, cooling to room temperature and separating supernate from solid residue; removing water from the supernate and mixing the residual substance with the dried solid residue, thereby acquiring a mixture B; adding citric acid-sodium citrate buffer solution into the mixture B; uniformly mixing and then adding lignocelluloses; performing enzymatic hydrolysis for 25-48 h at 30-40 DEG C, thereby completing the treatment of the enzymolysis saccharifying of lignocelluloses. According to the invention, the ultrasonic wave and the oil tea cake are combined for preprocessing the lignocelluloses so as to accelerate the enzymolysis saccharifying of lignocelluloses, intensify the activity of the lignocelluloses, save enzyme capacity and increase the enzymolysis efficiency.

The invention discloses an ultraviolet catalytic pretreatment method for degradation-resistant biomass. The method comprises the steps of structural treatment and nature regulation of the biomass. Prepared biomass suspension slurry or wastewater concentrated liquor is added into an ultraviolet catalytic reactor, then 1-10 g / L of oxidant is added, 0.1-0.8 MPa of compressed air is introduced for aeration and stirring, an electrodeless ultraviolet lamp is stimulated to emit ultraviolet light through microwaves, and the biomass in the biomass suspension slurry or wastewater is subjected to catalytic pretreatment under the synergetic action of the microwaves, the ultraviolet light and a catalyst. According to the method, multiple actions such as catalytic cracking, photo-catalytic oxidation and homogeneous catalytic oxidation occur simultaneously in a microwave electrodeless ultraviolet light catalyst system, so that reaction efficiency is high; the oxidation stage can be controlled by controlling pretreatment conditions, so that the treated biomass is suitable for microorganism utilization, and environment friendliness is achieved.

The invention relates to a method for synthesizing a biological adhesive, in particular to a method for synthesizing a soybean protein adhesive. Compared with the prior method, the method has the characteristics of short pretreatment time, short reaction time, and good water resistance, is simple, convenient and feasible, and also greatly improves the bonding strength of adhesives.

The present invention belongs to the technical field of industrial papermaking, and particularly relates to a preparation method of paper for industry. The method comprises: (1) raw material treatment, (2) soaking, (3) washing of paper, (4) pulp grinding treatment, and (5) screening and paper-making. According to the present invention, the method has characteristics of short material pretreatment time, low energy consumption and low equipment investment; the chemical agent is added to the raw materials, such that the yellowing resistance of the paper is enhanced, the storage time is substantially prolonged, the toughness and the softness of the paper can be increased, the smoothness and the antistatic capability of the paper surface are increased, and the water resistance and the moisture-proof property of the paper are improved; and the product can be widely used for printing paper, kraft paper, paper boards, liner paper and other craft paper.

The embodiment of the invention relates to the technical field of medical detection, and discloses a preparation method of a pretreatment reagent, the pretreatment reagent and a pretreatment method. The method comprises the steps of preparing a mixed solution, and adjusting the pH value of the mixed solution to a preset pH range; and taking a first preset quantity of the mixed solution as a lysis solution, sub-packaging the lysis solution in a centrifugal tube with a light shielding function, freeze-drying the centrifugal tube filled with the lysis solution so as to enable the lysis solution to form freeze-dried powder, and sealing and storing the freeze-dried powder to prepare the pretreatment reagent. The mixed solution comprises the following components: 0.2-1 mol / L of a buffer solution, 5-75 mg / mL of ascorbic acid, 5-50 [mu]g / mL of glutamine transpeptidase and 5-30 mol / L of a protein denaturant. Therefore, the freeze-dried powder obtained by freeze-drying the lysate comprising the components has good hemolysis treatment capacity, and the freeze-drying treatment time is short, namely the required pretreatment time is short. In addition, the lysate is stored in a centrifugal tube with a light shielding function in the form of freeze-dried powder, so that folic acid in a whole blood sample can be prevented from being exposed in light as far as possible.

The invention discloses functional fried rice noodles and a preparation method thereof. The preparation method comprises the following steps: soaking rice with water and jordaning to prepare rice slurry; adding xanthan gum and a fat-soluble polyphenol derivative after carrying out ultrasonic treatment, and applying high speed shearing for full mixing; carrying out techniques such as steam flaking,cooling, slitting and forming on the obtained mixed slurry to prepare the functional fried rice noodles. The preparation method disclosed by the invention has the advantages of simple operation and little loss of nutrients; by compounding a trace food additive, the functional fried rice noodles rich in slow digestion and anti-digestive starch can be prepared. The fried rice noodles can be used asa functional nutrition food for preventing the pathogenesis of chronic diseases such as diabetes mellitus and obesity.

The invention discloses an extraction method of flavor components of rapeseed oil, and belongs to the field of volatile flavor substance extraction. The invention provides the method for extracting volatile flavors of rapeseed oil by coupling probe type ultrasonic-assisted extraction and solvent extraction flavor evaporation combined technology. The method has the characteristics of non-thermal effect, low energy consumption, high efficiency, real extracted substances and the like, can obviously shorten the pretreatment time of the sample, and can identify more volatile components under the same qualitative conditions compared with solid-phase micro-extraction. The method for detecting the volatile components in the rapeseed oil, provided by the invention, is simple, efficient, green and environment-friendly, and has great application potential and economic value in the fields of rapeseed oil flavor component identification, evaluation and the like.

The invention relates to a method for extracting desoxyribonucleic acid in edible oil, which comprises the following steps of: adding desoxyribonucleic acid extract into the edible oil, and separating out an aqueous phase containing desoxyribonucleic acid after ultrasonic emulsification; and then adding a desoxyribonucleic acid precipitant into the aqueous phase, and precipitating the desoxyribonucleic acid. Because DNA in a sample is enriched by low-energy ultrasonic emulsification in the extracting process, the pretreatment time of the sample is shortened, time consumption is short, the extraction efficiency of the DNA is improved, cross contamination between the samples to be detected is avoided, and the extraction result is more accurate and reliable.

The invention belongs to the technical field of lignocellulose pretreatment, in particular to an FeOCl-based lignocellulose pretreatment system and application thereof. The pretreatment system consists of the following effective components including 0.2-3.5 g / L of FeOCl and 0.09-1.55 mol / L of peralcohol, wherein, the peralcohol is any one of hydrogen peroxide and sodium peroxide or a mixture of the hydrogen peroxide and the sodium peroxide in an arbitrary proportion; and the pH value of the pretreatment system is 3-8. The pretreatment system can have a Fenton-like catalytic reaction within thepH value range of 3-8; the treatment time is short; and the degradation efficiency of lignin and cellulose in a lignocellulose raw material is effectively improved.

The invention discloses a cutting seedling method of murraya paniculata. The cutting seedling method includes the steps of 1), clipping stems and branches of the murraya paniculate to obtain cutting slips 8-15cm in length and retaining at least two sections; 2), soaking lower cuts in water and a plant growth regulator sequentially; 3), plugging the cutting slips treated in the step 2) in soil and keeping air humidity of the grow environment to be 75wt% to 85wt%; 4), enabling the cutting slips in the step 3) to take root under light shading percentage of 70-80% at the temperature ranging 15DEG C-30DEG C, transplanting seedlings taken root to seed beds for cultivation, and after the seedlings grow up to 35cm, taking the seedlings out of the seed bed and performing field planting. The cutting seedling method has the advantages of short rooting cycle, high adaption, simple cultivation process and high breeding coefficient, purposes of fast growth, stable yield and high yield can be achieved, harvesting years are reduced, and sustainable and healthy development of wild murraya paniculata resources is sustained.

The invention discloses a kit and a detection method for detecting cyclosporine drug concentration in a dried blood spot sample, the kit comprises a kit body, a lining, a wide-mouth bottle, a first sample collection tube, a second sample collection tube and a third sample collection tube, the wide-mouth bottle is filled with a sample extraction liquid, the first sample collection tube is filled with a calibrator, and the second sample collection tube is filled with a sample extraction liquid. A quality control product is placed in the second sample collection tube, a blank quality control product is placed in the third sample collection tube, and glass fiber filter paper is arranged in the first sample collection tube, the second sample collection tube and the third sample collection tube and is used as a carrier of a standard product and the quality control product. The detection method based on the kit provided by the invention comprises the following steps: pretreating a sample, loading and detecting the sample by combining a liquid chromatography tandem mass spectrometer, and finally carrying out quantitative analysis on cyclosporine by adopting an internal standard method. According to the invention, the influence of hematocrit effect is avoided, the risk of occupational exposure is reduced, and the detection stability and the practicability of the kit are improved.

The invention discloses a method for improving the safety and quality of red dates. Cold plasma is used to pretreat the red dates, thereby reducing the hot air drying time of the red dates, improvingthe drying efficiency, reducing the drying energy consumption, and at the same time reducing the degradation of vitamin C, improving the antioxidant activity and reducing the generation of harmful product 5- hydroxymethyl furfural in the drying process. Therefore, the quality of the dried red dates is improved. The method mainly comprises the following steps to obtain the dried red dates: pretreating fresh red dates by using cold plasmas, and performing hot air drying treatment on the pretreated fresh red dates. The red dates are subjected to cold plasma pretreatment and then subjected to hotair drying. Compared with the method without pretreatment, the method of the invention can shorten the red date drying time and reduce the hot air drying energy consumption, and improve the safety andquality of the dried red dates. Compared with a chemical pretreatment method, the method has no problems of chemical residues and waste liquid treatment.

The invention discloses a method for extracting active ingredients of cistanche by adjusting osmotic pressure, which comprises the following steps: alternately spraying saline water and ethanol solution onto cistanche, transferring into a vacuum tank for vacuum treatment to break walls, soaking the cistanche subjected to wall breaking in ethanol for a period of time, grinding into thick liquid, and then carrying out ultrasonic oscillation on the slurry, filtering, purifying and drying to obtain the cistanche deserticola extract. According to the method disclosed by the invention, through alternate pretreatment of the saline water and the ethanol solution and vacuum wall breaking treatment, effective components in the cistanche are more easily extracted, and the extraction rate of the effective components of the cistanche is improved. Subsequent impurity removal treatment is carried out, so that the purity of the cistanche extract is ensured, and the cistanche extract with good quality can be obtained.

The invention relates to a pretreatment method of lignocelluloses, in particular to a pretreatment method of a straw lignocelluloses raw material, and aims to solve the technical problems of high catalyst preparation cost and high solvent cost of existing methods for degrading lignin by heteropoly acid. The method comprises the steps as follows: air-dried straw powder and a phosphotungstic acid aqueous solution are uniformly mixed and then subjected to two-stage heating, wherein the temperature at the first stage is 105 DEG C and the heating time is 1-4 h, and the temperature at the second stage is 130-160 DEG C and the heating time is 20-60 min; and then, suction filtration is performed, solid residues are pretreated straw lignocelluloses, and phosphotungstic acid is recovered after filtrate is collected. The method has the total sugar recovery rate of 70%-84% and catalyst recovery rate of 70%-80% and can be applied to the field of biomass pretreatment.

The invention relates to a method for quantitatively detecting a content of an antiviral drug in a plasma sample. The method comprises the following steps: (1) collecting trace peripheral blood; (2) mixing the peripheral blood whole blood sample with the protein precipitation solution containing an interior label, carrying out vortex and centrifugation, and diluting the supernatant to obtain a to-be-detected solution; and (3) detecting and analyzing the to-be-detected solution by adopting a high performance liquid chromatography-tandem mass spectrometry system. The invention provides the method for simultaneously detecting the vitamin A, the vitamin D2, the vitamin D3 and the vitamin E in the peripheral blood sample, the pretreatment is simple, the detection cost is low, and the detection efficiency is high.

The invention discloses a method for improving the extraction efficiency of cistanche by breaking cell walls of cistanche, and the method comprises the following steps: placing cistanche in a vacuum tank for vacuum treatment to break walls, and alternately spraying a mixed enzyme solution and an ethanol solution onto the cistanche to digest the cell walls and cell membranes; soaking the cistanche deserticola subjected to enzyme treatment in ethanol for a period of time, grinding into thick liquid, performing ultrasonic oscillation on the thick liquid, and performing filtering, purifying and drying to obtain the cistanche deserticola extract. According to the method, vacuum wall breaking, enzymolysis wall breaking and ethanol solubilization treatment are carried out on the cistanche, so that effective components in the cistanche are extracted more easily, and the extraction rate of the effective components of the cistanche is increased. And subsequent impurity removal treatment is carried out, so the purity of the cistanche extract is ensured, and the cistanche extract with good quality can be obtained.

The invention discloses an extraction material for quinolones in environmental water bodies and fishes and a corresponding detection method. The detection method comprises the following steps of: preparing magnetic Fe3O4 nanoparticles by a solvothermal reaction method; dispersing the magnetic Fe3O4 nanoparticles in a mixture of isopropanol, deionized water and concentrated ammoniacal liquor, and then adding tetraethoxysilane to prepare SiO2 / Fe3O4; dispersing the SiO2 / Fe3O4 in DMF(Dimethyl Formamide), and adding 2-aminoterephthalic acid; dissolving zinc acetate dihydrate in DMF , and dropwise adding the obtained solution into the solution obtained in the previous step, so as to obtain IRMOF-3 coated SiO2 / Fe3O4; adjusting a sample solution to a preset pH value by using diluted acetic acid and ammonium hydroxide, and adding the IRMOF-3 coated SiO2 / Fe3O4 for extraction; separating out a magnetic material by using a strong magnet, and eluting by using a solution; and analyzing the obtained product by using an LC-MS / MS (liquid chromatography-mass spectrometry / mass spectrometry) method. According to the method, the detection limit can be reduced by 3 orders of magnitude, and ciprofloxacin which cannot be detected by the Ministry of Agriculture 783 announcement 2-2006 can be detected by the method.

The invention relates to a pyromellitic dianhydride detection method based on Raman spectrum, which comprises the following steps: weighing 1 + / -0.05 g of pyromellitic dianhydride sample and dissolving in 100mL of anhydrous organic solvent to prepare a high-content pyromellitic dianhydride solution; performing Raman spectrum detection on the high-content pyromellitic dianhydride solution; origin integral is adopted to open Raman spectrum data, characteristic peak height or peak area data is fitted through a Gaussian function or a Lorentz function, a pyromellitic dianhydride Raman data model is established, and the pyromellitic dianhydride content in a sample is calculated through the pyromellitic dianhydride Raman data model. The method can avoid hydrolysis of pyromellitic dianhydride in the detection process, can directly detect the content of pyromellitic dianhydride, and is short in pretreatment time and simple to operate.

The invention relates to a method for extracting desoxyribonucleic acid in edible oil, which comprises the following steps of: adding desoxyribonucleic acid extract into the edible oil, and separating out an aqueous phase containing desoxyribonucleic acid after ultrasonic emulsification; and then adding a desoxyribonucleic acid precipitant into the aqueous phase, and precipitating the desoxyribonucleic acid. Because DNA in a sample is enriched by low-energy ultrasonic emulsification in the extracting process, the pretreatment time of the sample is shortened, time consumption is short, the extraction efficiency of the DNA is improved, cross contamination between the samples to be detected is avoided, and the extraction result is more accurate and reliable.