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Processing method for intensifying enzymolysis saccharifying of lignocelluloses

The technology of lignocellulase and lignocellulose is applied in the treatment field of strengthening lignocellulosic enzymatic hydrolysis and saccharification, can solve the problems of long pretreatment time, many chemical agents, not ideal, etc. Low and small steric hindrance effect

Active Publication Date: 2017-06-13
YULIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To sum up, these pretreatment methods all change the structure of lignocellulose to a certain extent, and strengthen the enzymatic hydrolysis efficiency of lignocellulose, but these processes are either extremely complicated, or the pretreatment time is long, or the addition of chemical Too many medicines lead to high cost, which is not ideal

Method used

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  • Processing method for intensifying enzymolysis saccharifying of lignocelluloses
  • Processing method for intensifying enzymolysis saccharifying of lignocelluloses
  • Processing method for intensifying enzymolysis saccharifying of lignocelluloses

Examples

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Effect test

Embodiment 1

[0036] 1) Remove floating impurities on the surface of the massive camellia oil cake, crush it through a 40-mesh sieve, and dry the sieved tea cake powder at 40°C to remove moisture; hybrid poplar sawdust is selected from a wood processing factory, remove floating impurities, and crush it through a 40-mesh sieve Sieve, and dry the sieved sawdust at 60°C to remove moisture.

[0037] 2) Mix the dry tea cake powder in (1) with the hybrid poplar sawdust in a mass ratio of 1:20, the total mass is 1g; then fully mix the mixture with deionized water in 1L for reaction according to the mass volume ratio of 1g:5mL In the device, it was treated under the action of ultrasonic wave (108W, 20.024kHz) for 10min. Then the reactant was cooled to room temperature, the supernatant was separated by filtration, and the solid residue was dried at 100°C for 2 hours; the supernatant was removed by membrane separation technology, and the obtained substance was mixed with the solid residue for enzymat...

Embodiment 2

[0046] 1) Lumpy camellia oleifera cake removes floating impurities on the surface, crushes through a 50-mesh sieve, and dries the sieved tea cake powder at 50°C to remove moisture; hybrid poplar sawdust is selected from a wood processing factory, removes floating impurities, and crushes through a 50-mesh sieve Sieve, and dry the sieved sawdust at 60°C to remove moisture.

[0047] 2) Mix the dry tea cake powder in (1) with the hybrid poplar sawdust at a mass ratio of 1:18, and the total mass is 1g; then fully mix the mixture with deionized water in 1L for reaction according to the mass-volume ratio of 1g:4mL In the device, it was treated under the action of ultrasonic wave (108W, 20.024kHz) for 20min. Then the reactant was cooled to room temperature, the supernatant was separated by filtration, and the solid residue was dried at 101°C for 2.5 hours; the supernatant was removed by membrane separation technology, and the obtained substance was mixed with the solid residue for enz...

Embodiment 3

[0056] 1) Lumpy camellia oleifera cake removes floating impurities on the surface, crushes through a 60-mesh sieve, and dries the sieved tea cake powder at 55°C to remove moisture; hybrid poplar sawdust is selected from a wood processing factory, removes floating impurities, and crushes through a 60-mesh sieve Sieve, and dry the sieved sawdust at 60°C to remove moisture.

[0057] 2) Mix the dry tea cake powder in (1) with the hybrid poplar sawdust in a mass ratio of 1:16, the total mass is 1g; then fully mix the mixture with deionized water in 1L according to the mass volume ratio of 1g:2.5mL In the reactor, it was treated under the action of ultrasonic waves (108W, 20.024kHz) for 30min. Then the reactant was cooled to room temperature, the supernatant was separated by filtration, and the solid residue was dried at 103°C for 3 hours; the supernatant was removed by membrane separation technology, and the obtained substance was mixed with the solid residue for enzymatic hydrolys...

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Abstract

The invention relates to a processing method for intensifying enzymolysis saccharifying of lignocelluloses. The method comprises the following steps: respectively screening oil tea cake and lignocelluloses biomass and then drying; mixing the dried oil tea cake and lignocelluloses biomass in a mass ratio of 1:(10-20), thereby acquiring a mixture A; mixing the mixture A with deionized water, thereby acquiring a reaction solution; performing ultrasonic treatment on the reaction solution; after ending the ultrasonic treatment, cooling to room temperature and separating supernate from solid residue; removing water from the supernate and mixing the residual substance with the dried solid residue, thereby acquiring a mixture B; adding citric acid-sodium citrate buffer solution into the mixture B; uniformly mixing and then adding lignocelluloses; performing enzymatic hydrolysis for 25-48 h at 30-40 DEG C, thereby completing the treatment of the enzymolysis saccharifying of lignocelluloses. According to the invention, the ultrasonic wave and the oil tea cake are combined for preprocessing the lignocelluloses so as to accelerate the enzymolysis saccharifying of lignocelluloses, intensify the activity of the lignocelluloses, save enzyme capacity and increase the enzymolysis efficiency.

Description

[0001] 【Technical field】 [0002] The invention belongs to the field of enzymatic hydrolysis of lignocellulose, and relates to a treatment method for enzymatic hydrolysis and saccharification of lignocellulose. [0003] 【Background technique】 [0004] With the increasingly serious problems of energy crisis and environmental pollution, countries all over the world have adopted various effective measures to encourage the development of biomass energy. Lignocellulose is an important renewable resource, which can be converted into energy and chemicals through effective pretreatment, microbial fermentation, enzymatic saccharification and other processes. However, lignocellulose has a complex structure, and cellulose, hemicellulose, and lignin are interrelated and intertwined with each other. The resulting steric hindrance makes it difficult for hydrolytic enzymes to directly act on the substrate. If the corresponding pretreatment technology is not used to destroy this dense structu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14
CPCC12P19/14C12P2201/00
Inventor 相玉琳王立鹏薛成虎郝华睿王若恺
Owner YULIN UNIV
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