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Method for extracting desoxyribonucleic acid in edible oil

A deoxyribonucleic acid and extraction method technology, which is applied in the field of deoxyribose nucleic acid extraction in edible oil, can solve the problems of complex processing technology, low accuracy rate and cumbersome process of refined vegetable edible oil, and achieve accurate and reliable extraction results with low consumption The effect of short time and safe experiment process

Active Publication Date: 2011-05-25
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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Problems solved by technology

[0003] The processing technology of refined vegetable edible oil is complicated, and the final product contains only a very small amount of DNA
Traditional DNA extraction methods used in edible oils, such as the CTAB (cetyltrimethylammonium bromide) method, require long-term mechanical stirring of the sample, which takes a long time, is inefficient, and the process is cumbersome and the accuracy is low. high

Method used

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  • Method for extracting desoxyribonucleic acid in edible oil
  • Method for extracting desoxyribonucleic acid in edible oil
  • Method for extracting desoxyribonucleic acid in edible oil

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Embodiment Construction

[0021] The method for extracting DNA in edible oil will be described in further detail below mainly in conjunction with the accompanying drawings and specific examples.

[0022] The extraction method of deoxyribonucleic acid in the edible oil of one embodiment, comprises the steps:

[0023] Step S1: Add the preheated DNA extraction solution to the edible oil, vibrate vigorously to disperse the DNA extraction solution evenly in the edible oil, and then ultrasonically emulsify to obtain a uniformly dispersed emulsion.

[0024] For different types of genetically modified edible oils, such as genetically modified soybean edible oil, genetically modified blended oil, genetically modified rapeseed oil, etc., different volumes of samples to be tested can be measured. If the volume is too small, the DNA content contained in it is too low. Detection; the volume is too large, which is not conducive to the experimental operation and causes waste. Preferably, for commercially available s...

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Abstract

The invention relates to a method for extracting desoxyribonucleic acid in edible oil, which comprises the following steps of: adding desoxyribonucleic acid extract into the edible oil, and separating out an aqueous phase containing desoxyribonucleic acid after ultrasonic emulsification; and then adding a desoxyribonucleic acid precipitant into the aqueous phase, and precipitating the desoxyribonucleic acid. Because DNA in a sample is enriched by low-energy ultrasonic emulsification in the extracting process, the pretreatment time of the sample is shortened, time consumption is short, the extraction efficiency of the DNA is improved, cross contamination between the samples to be detected is avoided, and the extraction result is more accurate and reliable.

Description

【Technical field】 [0001] The invention relates to a chemical separation process, in particular to a method for extracting deoxyribonucleic acid (DNA) in edible oil. 【Background technique】 [0002] With the development of transgenic technology, various genetically modified products have gradually entered people's lives. On March 20, 2002, China began to implement the "Administrative Measures for the Labeling of Agricultural Genetically Modified Organisms", which stipulates that the country implements an inspection, quarantine and labeling system for agricultural genetically modified organisms. In 2004, China approved the import of herbicide-tolerant genetically modified soybeans (GMO Roundup Ready soybeans), and various genetically modified soybean-related products began to enter the Chinese market in an all-round way. By the end of May 2008, China had imported 9.0×10 transgenic soybeans 6 tons or so. Genetically modified organisms have good market competitiveness and appl...

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Application Information

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IPC IPC(8): C07H21/04C07H1/08
Inventor 潘建青吴慧英王立平
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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