177results about How to "Prolong blood circulation time" patented technology

The invention provides a targeted photothermal black phosphorus nano-preparation, which is prepared from a black phosphorus nano-slice, polyethylene glycol absorbed on the surface of the black phosphorus nano-slice by means of electrostatic attraction, and folic acid connected to the polyethylene glycol by means of an amide bond, wherein one end of the polyethylene glycol is amino, and the other end of the polyethylene glycol is imino; nitrogen atom of the imino and carbonyl carbon atom of the folic acid are connected with each other so as to form the amide bond; the folic acid is exposed at the outermost layer of the nano-preparation. The targeted photothermal black phosphorus nano-preparation is high in stability, good in target recognition ability and excellent in light-heat property, is capable of effectively killing cancer cells, and can be used for performing targeted photothermal treatment on cancers at the cellular level. The invention also provides a preparation method and application of the targeted photothermal black phosphorus nano-preparation.

The invention relates to a nanomicelle of magnetic tumor double-targeting polymer and a preparation method thereof. The nanomicelle of magnetic tumor double-targeting polymer is a nuclear shell structure; the surface of an outer shell is connected with targeted ligand, an inner core is enclosed with nano-particles of super paramagnetic ferriferrous oxide and anticarcinogen with hydrophobic property; in virtue of the physical effect of an external magnetic field and induction of the targeting ligand, the tumor double-targeting function of the nanomicelle is realized. The invention also provides the preparation method of the nanomicelle of magnetic tumor double-targeting polymer: polyethylene glycol with end capping by folacin, and poly Epsilon-caprolactone or sandwich copolymer of poly propiolactone are taken as raw materials and enclosed with the nano-particles of super paramagnetic ferriferrous oxide and the anticarcinogen with hydrophobic property, and then dialysed to obtain the nanomicelle of magnetic tumor double-targeting polymer. The drug carrier system of the nanomicelle reinforces the treatment effect of the anticarcinogen with hydrophobic property on tumor, prolongs the circulating time of the anticarcinogen in human body, intensifies the targeting action of drugs, improves the release efficiency and partial concentration of drugs, and reduces the dosage and toxic and side effect of drugs.

The invention relates to the field of biologic medicines, and discloses an albumin indocyanine green paclitaxel compound as well as a preparation method and an application thereof. The albumin indocyanine green paclitaxel compound consists of indocyanine green, paclitaxel and human serum albumin, wherein the indocyanine green and the paclitaxel are absorbed onto human serum albumin through a hydrophobic force. The compound is good in water solubility, biocompatibility and dispersity, can generate heat in laser radiation, can promote more material cells, can kill tumor cells, is further high in fluorescence property, and can instruct joint treatment of photo-thermal therapy and chemotherapy in a fluorescence imaging mode. The preparation of the albumin indocyanine green paclitaxel compound is simple to operate and can be applied to on-scale preparation of the albumin indocyanine green paclitaxel compound, and the raw materials are easily available.

The invention belongs to the field of pharmaceutical preparations, and relates to preparation of a novel target nano carrier and application of the novel target nano carrier in a camptothecin drug targeting delivery system. The system can be represented as PAMAM-HA/drug, wherein PAMAM is polyamide amine-amine arboraceous macromolecules of different generations, HA is hyaluronic acid of different molecular weights, and the drug is one or more of camptothecin anticancer drugs such as camptothecin, 10- hydroxycamptothecine, topotecan and the like. The carrier is used for wrapping the camptothecin drugs, and has the actions of prolonging the drug blood circulation time, improving the drug bioavailability, reducing the non-tumor part concentration of the drug, reducing the drug cytotoxicity, and simultaneously protecting the lactonic rings of the camptothecin drugs from damage, thereby improving the tumor treatment efficiency from multiple aspects. The nano carrier provided by the invention has important significance in promoting the safe delivery of the camptothecin drugs and the effective treatment of tumor.

The present invention relates to a quercetin long-acting liposome powder injection and its preparation method. Said powder injection is formed from quercetin, polyethylene glycol-ethanolamine phosphoglycerides, lecithin, cholesterol and excipient. Said invention also provides the concrete steps of its preparation method.

The invention relates to the technical field of biomedicine, in particular to a hydrogen peroxide-responsive liposomal nanoprobe and a preparation method and application thereof. The liposomal nanoprobe comprises a liposome, HRP and ABTS, wherein a hydrophilic cavity of the liposome is loaded with HRP, and a hydrophobic layer of the liposome is loaded with ABTS. The hydrogen peroxide-responsive liposomal nanoprobe prepared by the invention has very good hydrogen peroxide responsiveness, and can realize the detection of nano hydrogen peroxide. Compared with the traditional method for detecting hydrogen peroxide by fluorescence or bioluminescence, the photoacoustic imaging detection can significantly improve the depth of penetration of tissues to achieve the hydrogen peroxide imaging of deep tissues; the photoacoustic imaging of the liposome nanoprobe can monitor the occurrence and development of inflammation in real time, and the liposome nanoprobe can react with hydrogen peroxide in a tumor microenvironment to achieve the photoacoustic imaging of tumors.

The invention discloses an acid-responsive nanometer micelle for drug loading, a preparation method and an application thereof. The nano-micelles were self-assembled from hydrophilic segment polyethylene glycol (PEG) and hydrophobic segment pH-sensitive polyaspartyl diisopropylethylenediamine/di-n-butylethylenediamine (PAsp (DIP/DBA)). The nano-micelles can prolong the drug circulation time, aggregate in the tumor site, increase the local drug concentration, and respond to the micro-acid environment of the tumor tissue. As a drug carrier of stimulation response, the nano-micelles can rapidly release the loaded chemotherapeutic drug adriamycin in the tumor site, and play a significant anti-tumor effect. At the same time, the nano-micelles loaded with magnetic resonance contrast agent superparamagnetic iron oxide can be used for tumor magnetic resonance imaging and monitoring drug uptake and aggregation. This method utilizes nano-drug aggregation at tumor site and acid responsiveness ofcarrier to realize rapid drug release to improve tumor therapeutic effect, and endows nano-micellar MRI visualization function, which provides a promising innovative strategy for cancer diagnosis andtreatment, and has broad application prospects.

The invention relates to a multi-mode targeted probe for early hepatic fibrosis diagnosis and a preparation method thereof. The multi-mode targeted probe for the early hepatic fibrosis diagnosis is characterized by comprising a vector, and the vector is connected with a fluorescence group and a magnetic resonance imaging group and can be connected with a targeted compound group, which can be combined with a receptor on the surface of a hepatic stellate cell in hepatic fibrosis/cirrhosis, through a bridging group. The preparation method comprises the steps of: connecting the targeted compound group, which can be combined with the receptor on the surface of the hepatic stellate cell in the hepatic fibrosis/cirrhosis, with the bridging group, and sequentially connecting the targeted compoundgroup, the fluorescence group and the magnetic resonance imaging group on the vector. The targeted probe has higher sensitivity when being used for diagnosing and evaluating the fibrosis diagnosis.

The invention relates to a functionalized dendrimer-based SPECT-CT bimodal imaging contrast agent and a preparation method thereof. The functionalized dendrimer-based SPECT-CT bimodal imaging contrast agent is obtained by pegylation modified fifth polyamide-amine dendrimer as a polymer carrier material; connecting diethylenetriaminepentaacetic acid on the dendrimer surface through covalent grafting; coating gold nanoparticles through an in-situ synthesis method; carrying out acetylation or hydroxylation on residual surface amino groups; and marking <99m>Tc. The functionalized dendrimer-based SPECT-CT bimodal imaging contrast agent has a very good SPECT/CT imaging effect, realizes SPECT/CT imaging of different in-vivo tissue sites of a mouse, lays good foundation for development of multi-functional tissue specific contrast agent, and has wide application prospects.

The invention belongs to the field of pharmaceutic preparations, and discloses the preparation and pharmaceutical application of a protein adsorption-resistant photic sensitivity-enhanced anoxic stress cationic carrier. A cationic polymer is taken as a skeleton to modify a zwitter-ion glycine betaine monomer so as to weaken the adsorption to protein in an organism of the polymer; further modifyingthe intermediate with a hydrophobic connecting arm containing an anoxic stress azobenzene structure and a photosensitizer, so as to construct an amphipathic polymer carrier. The cationic modifier form a nanoparticle through self assembling in a water solution, can be directly applied to photodynamics therapy, can also be used for loading hydrophobic chemical drugs or nucleic acid drugs and applied to photodynamics and antineoplastic drug combination therapy. According to the invention, the photodynamics therapy of the photosensitizer is used for constructing a high-anoxic tumor microenvironment to stimulate nanometer disassembly, thus promoting drug release and improving the utilization ratio; in addition, through the sensitivity-enhanced antineoplastic drug therapeutic effect generated by the photodynamic active oxygen, the high-efficient synergistic treatment on tumour is realized.

The invention belongs to the field of medical imaging contrast agents, and provides a pegylated polyethyleneimine macromolecular magnetic resonance imaging contrast agent and a preparation method of the contrast agent. The preparation method comprises the following steps: first, modifying a gadolinium ion chelating agent DTPA (Diethylenetriamine Pentaacetic Acid) on the surface of PEI (Polyethyleneimine) to prepare PEI-DPTA; second, modifying a composite material by pegylation to prepare PEI-DTPA-mPEG; and finally, chelating gadolinium ions to prepare PEI-DTPA (Gd III)-mPEG, and acetylating residual amino groups on the surface of PEI. The pegylated polyethyleneimine macromolecular magnetic resonance imaging contrast agent obtained by the invention realizes in vivo MR (Magnetic Resonance) blood vessel and kidney imaging of a mouse as well as passive targeted MR imaging detection of tumor tissues. The contrast agent is wide in application prospect.

The invention provides a preparation method of convertible surface charge nano-gel. The preparation method includes the steps: A) dissolving an initiating agent with a morpholinyl group and L-glutamic acid polyethylene glycol single methyl ether-N-carboxyanhydride into an organic solvent for reaction to obtain mixed solution; B) mixing a compound with a formula (I) structure, a compound with a formula (II) structure and the mixed solution obtained in the step A) for reaction to obtain reaction solution; C) mixing and filtering the reaction solution obtained in the step B) and the organic solvent to obtain the convertible surface charge nano-gel. A convertible surface charge nano-gel drug-loaded particle has no toxic and side effects on human bodies and has excellent water solubility, stability and biocompatibility.

The invention relates to the technical field of preparing a contrast agent by utilizing a hyperbranched polymer. A preparing method of the hyperbranched polymer includes reacting a lysine salt and caustic alkali in a mole ratio of 100:80-90 at 140-160 DEG C for 40-50 h in a protective atmosphere; mixing a hyperbranched polylysine skeleton and diethylenetriaminepentaacetic acid according to a mass ratio of at least 1:5 in water; reacting the mixture at room temperature for 6-8 h; mixing the first hyperbranched polylysine molecule and a gadolinium compound in water; reacting the mixture at 37-42 DEG C for 6-12 h, with the mass ratio of the first hyperbranched polylysine molecule to the gadolinium compound being 1:1-1:2; and mixing and reacting the second hyperbranched polylysine molecule and folic acid in a carbonate the pH value of which is 8-10 for 5-12 h. The hyperbranched polylysine contrast agent has good biocompatibility, low toxicity and a high relaxation rate and can be metabolized in vivo.

The invention relates to a preparation method of tree-like macromolecular and stable ultra-small ferroferric oxide/gold nano-flowers. The preparation method comprises the following steps: mixing FeCl3, sodium citrate and anhydrous sodium acetate and reacting to obtain ultra-small Fe3O4NPs; carrying out activation treatment to obtain activated ultra-small Fe3O4NPs; enabling a G5 solution to react with a chloroauric acid solution to obtain G5-Au NPs; enabling the G5-Au NPs to react with the ultra-small Fe3O4NPs, so as to obtain G5-Fe3O4-Au NPs; enabling the chloroauric acid solution, an AgNO3 solution and an ascorbic acid AA solution to react, so as to obtain a G5-Fe3O4-AuNFs solution; carrying out acetylation treatment to obtain a product. Nanoparticles prepared by the preparation method have excellent biocompatibility and a good MR/CT/PA imaging function; the nanoparticles can be used for photothermal therapy of tumors; a photothermal therapy effect is improved through radiotherapy andthe nanoparticles can be used for a potential tumor multi-modal imaging contrast agent and combined therapy and have a wide application prospect.

Using liposomes to deliver bioactive agents to cancer or tumor cells and compositions of specific lipids that form liposomes to deliver a biologically active agent.