The application provides a
polysaccharide of an organic black
fungus and a preparation method of a particle thereof. The method comprises the following steps: Step 1, taking a sporocarp of the organic black
fungus,
drying the sporocarp, and then crushing the sporocarp to obtain organic black
fungus dry powder; Step 2, mixing the organic black fungus
dry powder with water and infiltrating the organic black fungus
dry powder to obtain an extracting solution; Step 3, carrying out
microwave and ultrasonic extraction on the extracting solution in the Step 2, and taking a supernatant solution for later use; Step 4, taking the supernatant solution in the Step 3, removing albumen and discoloring the supernatant solution; Step 5, adding
ethanol into a product in the Step 4, carrying out
alcohol precipitation, and filtering and washing to obtain a
polysaccharide coarse product; Step 6, purifying the
polysaccharide coarse product in the Step 5 by high-speed
countercurrent chromatography to obtain the polysaccharide of the organic black fungus. In the preparation method, a polysaccharide component of a black fungus is extracted by a method of combining
multiple methods such as an ultrasonic extraction method, a
microwave extraction method, a
water extraction and
alcohol precipitation method, a high-speed countercurrent chromatographic method and the like; the purity of the polysaccharide is high; the extraction rate is high; an
organic solvent used in a process is few, is not remained, and has no potential
hazard; the polysaccharide is safe to eat; the time of the process is short; the cost is low.