Polysaccharide of organic black fungus and preparation method of particle thereof

Abstract

The application provides a polysaccharide of an organic black fungus and a preparation method of a particle thereof. The method comprises the following steps: Step 1, taking a sporocarp of the organic black fungus, drying the sporocarp, and then crushing the sporocarp to obtain organic black fungus dry powder; Step 2, mixing the organic black fungus dry powder with water and infiltrating the organic black fungus dry powder to obtain an extracting solution; Step 3, carrying out microwave and ultrasonic extraction on the extracting solution in the Step 2, and taking a supernatant solution for later use; Step 4, taking the supernatant solution in the Step 3, removing albumen and discoloring the supernatant solution; Step 5, adding ethanol into a product in the Step 4, carrying out alcohol precipitation, and filtering and washing to obtain a polysaccharide coarse product; Step 6, purifying the polysaccharide coarse product in the Step 5 by high-speed countercurrent chromatography to obtain the polysaccharide of the organic black fungus. In the preparation method, a polysaccharide component of a black fungus is extracted by a method of combining multiple methods such as an ultrasonic extraction method, a microwave extraction method, a water extraction and alcohol precipitation method, a high-speed countercurrent chromatographic method and the like; the purity of the polysaccharide is high; the extraction rate is high; an organic solvent used in a process is few, is not remained, and has no potential hazard; the polysaccharide is safe to eat; the time of the process is short; the cost is low.

Description

technical field [0001] The present application relates to a method for preparing polysaccharides, more specifically, to a method for preparing organic black fungus polysaccharides and granules thereof. Background technique [0002] The fruit body of organic black fungus is gelatinous, disc-shaped, irregular in shape, and 3-12 cm in diameter. Soft when fresh, horny when dry. It has a delicate taste and a special flavor. It is a well-known edible fungus rich in nutrition. [0003] The fruit body of organic black fungus is gelatinous, disc-shaped, irregular in shape, and 3-12 cm in diameter. Soft when fresh, horny when dry. It has a delicate taste and a special flavor. It is a well-known edible fungus rich in nutrition. Dried black fungus contains carbohydrates, 10.6 grams of protein, 0.2 grams of fat, 306 calories of heat, amino acids, vitamins and minerals. The protein of water hair fungus is extremely low, containing 1.5 grams per 100 grams. It has the effects of benef...

AI Technical Summary

Problems solved by technology

[0020] In view of this, the technical problem to be solved in this application is to overcome the shortcomings of the existing technology, adopt the idea of ​​combining multiple extraction methods, use microwave extraction, ultrasonic extraction, water extraction and alcohol precipitation, high-speed countercurrent chromatography, etc. The combination of the two methods can improve the extraction rate to a certain extent, reduce the time-consuming process, and reduce the cost