48results about How to "Increase endocytosis" patented technology

Compositions and methods for transport or release of therapeutic and diagnostic agents or metabolites or other analytes from cells, compartments within cells, or through cell layers or barriers are described. The compositions include a membrane barrier transport enhancing agent and are usually administered in combination with an enhancer and / or exposure to stimuli to effect disruption or altered permeability, transport or release. In a preferred embodiment, the compositions include compounds which disrupt endosomal membranes in response to the low pH in the endosomes but which are relatively inactive toward cell membranes (at physiologic pH, but can become active toward cell membranes if the environment is acidified below ca. pH 6.8), coupled directly or indirectly to a therapeutic or diagnostic agent. Other disruptive agents can also be used, responsive to stimuli and / or enhancers other than pH, such as light, electrical stimuli, electromagnetic stimuli, ultrasound, temperature, or combinations thereof. The compounds can be coupled by ionic, covalent or H bonds to an agent to be delivered or to a ligand which forms a complex with the agent to be delivered. Agents to be delivered can be therapeutic and / or diagnostic agents. Treatments which enhance delivery such as ultrasound, iontopheresis, and / or electrophereis can also be used with the disrupting agents.

The invention discloses a dopamine ligand-containing two-block polymer, a synthesis method and application thereof. The polymer uses polyethylene glycol as a hydrophilic end, and phenylboronic acid-modified polyethylene glycol is used as a targeting ligand. With glutamate and glutamate dopamine as repeating units as hydrophobic ends. The dopamine ligand-containing diblock polymer prepared by the invention can be used to encapsulate doxorubicin to form drug-loaded nano micelles, and then add iron ions to form coordination bonds between dopamine and iron ions for cross-linking to form nuclear cross-linking Doxorubicin-loaded nanomicelles. The nuclear cross-linked micelle prepared by the invention is simple and convenient in preparation method, and has high drug encapsulation rate, the micelle can be kept stable in the process of blood circulation, and can realize pH-sensitive drug release in tumor cell lysosomes.